{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:57:42Z","timestamp":1790870262238,"version":"4.1.0"},"reference-count":41,"publisher":"The Endocrine Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,8,1]]},"abstract":"<jats:p>We have examined the effects of acute administration of the cannabinoid receptor type 1 (CB1) antagonist AM251 on the rat hypothalamic-pituitary-adrenal (HPA) axis with respect to both gender and time of day. Blood samples were collected from conscious male and female rats every 5 min using an automated blood sampling system, and corticosterone concentrations were determined. In male rats, there was a distinct diurnal effect of AM251 with a greater activation of the HPA axis in the morning (diurnal trough) compared with the evening (diurnal peak). At both times of the day, circulating corticosterone concentrations were elevated for approximately 4 h after AM251 administration. In female rats, there was also diurnal variation in the activation of the HPA axis; however, these effects were not as profound as those in males. Corticosterone concentrations were only slightly elevated at the diurnal trough and for a shorter time period than in males (2 compared with 4 h). Moreover, there was no effect of AM251 on corticosterone concentrations when administered at the diurnal peak. Subsequent studies, only in males, in which both ACTH and corticosterone were measured, confirmed that the effects of AM251 on corticosterone were mediated by ACTH. Moreover, the elevation of both ACTH and corticosterone could be replicated using another CB1 antagonist, AM281. These data demonstrate that the extent and duration of HPA axis activation after CB1 blockade are clearly dependent on both gender and time of day.<\/jats:p>","DOI":"10.1210\/en.2010-0101","type":"journal-article","created":{"date-parts":[[2010,6,10]],"date-time":"2010-06-10T01:11:27Z","timestamp":1276132287000},"page":"3720-3727","source":"Crossref","is-referenced-by-count":50,"title":["Regulation of the Hypothalamic-Pituitary-Adrenal Axis Circadian Rhythm by Endocannabinoids Is Sexually Diergic"],"prefix":"10.1210","volume":"151","author":[{"given":"Helen C.","family":"Atkinson","sequence":"first","affiliation":[{"name":"Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Bristol BS1 3NY, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James D.","family":"Leggett","sequence":"first","affiliation":[{"name":"Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Bristol BS1 3NY, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan A.","family":"Wood","sequence":"first","affiliation":[{"name":"Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Bristol BS1 3NY, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma S.","family":"Castrique","sequence":"first","affiliation":[{"name":"Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Bristol BS1 3NY, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne M.","family":"Kershaw","sequence":"first","affiliation":[{"name":"Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Bristol BS1 3NY, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stafford L.","family":"Lightman","sequence":"first","affiliation":[{"name":"Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Bristol BS1 3NY, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2010,6,9]]},"reference":[{"key":"2020071613154740000_R1","doi-asserted-by":"crossref","first-page":"696","DOI":"10.1111\/j.1749-6632.1969.tb14008.x","article-title":"Dynamic asymmetries in the corticosteroid feedback path and distribution-metabolism-binding elements of the adrenocortical system.","volume":"156","author":"Dallman","year":"1969","journal-title":"Ann NY Acad Sci"},{"key":"2020071613154740000_R2","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1002\/0470870796.ch14","article-title":"Significance of pulsatility in the HPA axis.","volume":"227","author":"Lightman","year":"2000","journal-title":"Novartis Found Symp"},{"key":"2020071613154740000_R3","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1210\/endo.139.2.5857","article-title":"Ultradian, circadian, and stress-related hypothalamic-pituitary-adrenal axis activity: a dynamic digital-to-analog modulation.","volume":"139","author":"Chrousos","year":"1998","journal-title":"Endocrinology"},{"key":"2020071613154740000_R4","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1677\/JOE-07-0378","article-title":"Circadian clocks: regulators of endocrine and metabolic rhythms.","volume":"195","author":"Hastings","year":"2007","journal-title":"J Endocrinol"},{"key":"2020071613154740000_R5","doi-asserted-by":"crossref","first-page":"2344","DOI":"10.1126\/science.289.5488.2344","article-title":"Resetting of circadian time in peripheral tissues by glucocorticoid signaling.","volume":"289","author":"Balsalobre","year":"2000","journal-title":"Science"},{"key":"2020071613154740000_R6","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1177\/0748730403261894","article-title":"Influence of the corticosterone rhythm on photic entrainment of locomotor activity in rats.","volume":"19","author":"Sage","year":"2004","journal-title":"J Biol Rhythms"},{"key":"2020071613154740000_R7","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1002\/glia.20613","article-title":"Ultrastructural plasticity in the rat suprachiasmatic nucleus. 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