{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:28:10Z","timestamp":1791282490221,"version":"4.1.0"},"reference-count":47,"publisher":"The Endocrine Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:p>Genome analysis of patients with disorders of sex development, and gain- and loss-of-function studies in mice indicate that gonadal development is regulated by opposing signals. In females, the Wnt\/\u03b2-catenin canonical pathway blocks testicular differentiation by repressing the expression of the Sertoli cell-specific gene Sox9 by an unknown mechanism. Using cell and embryonic gonad culture models, we show that activation of the Wnt\/\u03b2-catenin pathway inhibits the expression of Sox9 and Amh, whereas mRNA and protein levels of Sry and steroidogenic factor 1 (Sf1), two key transcriptional regulators of Sox9, are not altered. Ectopic activation of Wnt\/\u03b2-catenin signaling in male gonads led to a loss of Sf1 binding to the Tesco enhancer and absent Sox9 expression that we also observed in wild-type ovaries. Moreover, ectopic Wnt\/\u03b2-catenin signaling induced the expression of the female somatic cell markers, Bmp2 and Rspo1, as a likely consequence of Sox9 loss. Wnt\/\u03b2-catenin signaling in XY gonads did not, however, affect gene expression of the steroidogenic Leydig cell Sf1 target gene, Cyp11a1, or Sf1 binding to the Cyp11a1 promoter. Our data support a model in ovary development whereby activation of \u03b2-catenin prevents Sf1 binding to the Sox9 enhancer, thereby inhibiting Sox9 expression and Sertoli cell differentiation.<\/jats:p>","DOI":"10.1210\/en.2011-1347","type":"journal-article","created":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T14:28:30Z","timestamp":1322749710000},"page":"901-912","source":"Crossref","is-referenced-by-count":74,"title":["Wnt Signaling in Ovarian Development Inhibits Sf1 Activation of Sox9 via the Tesco Enhancer"],"prefix":"10.1210","volume":"153","author":[{"given":"Pascal","family":"Bernard","sequence":"first","affiliation":[{"name":"Prince Henry's Institute of Medical Research (P.B., J.R., H.S., D.P.C., V.R.H.), Clayton, Melbourne 3168, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janelle","family":"Ryan","sequence":"additional","affiliation":[{"name":"Prince Henry's Institute of Medical Research (P.B., J.R., H.S., D.P.C., V.R.H.), Clayton, 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