{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:05:53Z","timestamp":1791273953100,"version":"4.1.0"},"reference-count":37,"publisher":"The Endocrine Society","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,9,1]]},"abstract":"<jats:p>The G protein \u03b1-subunit Gs\u03b1 mediates receptor-stimulated cAMP generation. Heterozygous inactivating Gs\u03b1 mutations on the maternal allele result in obesity primarily due to reduced energy expenditure in Albright hereditary osteodystrophy patients and in mice. We previously showed that mice with central nervous system (CNS)-specific Gs\u03b1 deletion on the maternal allele (mBrGs KO) also develop severe obesity with reduced energy expenditure and that Gs\u03b1 is primarily expressed from the maternal allele in the paraventricular nucleus (PVN) of the hypothalamus, an important site of energy balance regulation. We now generated mice with PVN-specific Gs\u03b1 deficiency by mating Single-minded 1-cre and Gs\u03b1-floxed mice. Homozygous Gs\u03b1 deletion produced early lethality. Heterozygotes with maternal Gs\u03b1 deletion (mPVNGsKO) also developed obesity and had small reductions in energy expenditure. However, this effect was much milder than that found in mBrGsKO mice and was more prominent in males. We previously showed mBrGsKO mice to have significant reductions in melanocortin receptor agonist-stimulated energy expenditure and now show that mBrGsKO mice have impaired cold-induced brown adipose tissue stimulation. In contrast, these effects were absent in mPVNGsKO mice. mPVNGsKO mice also had minimal effects on glucose metabolism as compared with mBrGsKO mice. Consistent with the presence of Gs\u03b1 imprinting, paternal heterozygotes showed no changes in energy or glucose metabolism. These results indicate that although Gs\u03b1 deficiency in PVN partially contributes to the metabolic phenotype resulting from maternal Gs\u03b1 mutations, Gs\u03b1 imprinting in other CNS regions is also important in mediating the CNS effects of Gs\u03b1 mutations on energy and glucose metabolism.<\/jats:p>","DOI":"10.1210\/en.2012-1113","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T07:42:39Z","timestamp":1340782959000},"page":"4256-4265","source":"Crossref","is-referenced-by-count":51,"title":["Gs\u03b1 Deficiency in the Paraventricular Nucleus of the Hypothalamus Partially Contributes to Obesity Associated with Gs\u03b1 Mutations"],"prefix":"10.1210","volume":"153","author":[{"given":"Min","family":"Chen","sequence":"first","affiliation":[{"name":"Signal Transduction Section, Metabolic Diseases Branch (M.C., A.B., J.Z., L.S.W.),Bethesda, Maryland 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