{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:44:50Z","timestamp":1790423090828,"version":"4.1.0"},"reference-count":50,"publisher":"The Endocrine Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>KNDy (kisspeptin\/neurokinin B\/dynorphin) neurons of the arcuate nucleus (ARC) appear to mediate the negative feedback actions of estradiol and are thought to be key regulators of pulsatile LH secretion. In the ewe, KNDy neurons may also be involved with the positive feedback actions of estradiol (E2) to induce the LH surge, but the role of kisspeptin neurons in the preoptic area (POA) remains unclear. The goal of this study was to identify which population(s) of kisspeptin neurons is (are) activated during the LH surge and in response to the removal of E2-negative feedback, using Fos as an index of neuronal activation. Dual-label immunocytochemistry for kisspeptin and Fos was performed on sections containing the ARC and POA from ewes during the luteal phase of the estrous cycle, or before or after the onset of the LH surge (experiment 1), and from ovary-intact, short-term (24 h) and long-term (&amp;gt;30 d) ovariectomized (OVX) ewes in anestrus (experiment 2). The percentage of kisspeptin neurons expressing Fos in both the ARC and POA was significantly higher during the LH surge. In contrast, the percentage of kisspeptin\/Fos colocalization was significantly increased in the ARC, but not POA, after both short- and long-term E2 withdrawal. Thus, POA kisspeptin neurons in the sheep are activated during, and appear to contribute to, E2-positive feedback, whereas ARC kisspeptin (KNDy) neurons are activated during both surge and pulsatile modes of secretion and likely play a role in mediating both positive and negative feedback actions of E2 on GnRH secretion in the ewe.<\/jats:p>","DOI":"10.1210\/en.2012-1357","type":"journal-article","created":{"date-parts":[[2012,9,19]],"date-time":"2012-09-19T07:43:14Z","timestamp":1348040594000},"page":"5406-5414","source":"Crossref","is-referenced-by-count":123,"title":["KNDy (Kisspeptin\/Neurokinin B\/Dynorphin) Neurons Are Activated during Both Pulsatile and Surge Secretion of LH in the Ewe"],"prefix":"10.1210","volume":"153","author":[{"given":"Christina M.","family":"Merkley","sequence":"first","affiliation":[{"name":"Graduate Program in Neuroscience (C.M.M.), University of Western Ontario, London, Ontario, Canada, N6A 5C1"},{"name":"Department of Molecular and Integrative Physiology and The Reproductive Sciences Program (C.M.M., L.M.C., M.N.L.), University of Michigan, Ann Arbor, Michigan 48109-1382"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrina L.","family":"Porter","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology (K.L.P., S.M.H., H.J.B., S.D., R.L.G.), West Virginia University, Morgantown, West Virginia 26506-9229"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lique M.","family":"Coolen","sequence":"first","affiliation":[{"name":"Department of Psychology (L.M.C.), University of Michigan, Ann Arbor, Michigan 48109-1043"},{"name":"Department of Molecular and Integrative Physiology and The Reproductive Sciences Program (C.M.M., L.M.C., M.N.L.), University of Michigan, Ann Arbor, Michigan 48109-1382"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley M.","family":"Hileman","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology (K.L.P., S.M.H., H.J.B., S.D., R.L.G.), West Virginia University, Morgantown, West Virginia 26506-9229"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather J.","family":"Billings","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology (K.L.P., S.M.H., H.J.B., S.D., R.L.G.), West Virginia University, Morgantown, West Virginia 26506-9229"},{"name":"Department of Neurobiology & Anatomy (H.J.B.), West Virginia University, Morgantown, West Virginia 26506-9128"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Drews","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology (K.L.P., S.M.H., H.J.B., S.D., R.L.G.), West Virginia University, Morgantown, West Virginia 26506-9229"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Goodman","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology (K.L.P., S.M.H., H.J.B., S.D., R.L.G.), West Virginia University, Morgantown, West Virginia 26506-9229"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael N.","family":"Lehman","sequence":"first","affiliation":[{"name":"Department of Molecular and Integrative Physiology and The Reproductive Sciences Program (C.M.M., L.M.C., M.N.L.), University of Michigan, Ann Arbor, Michigan 48109-1382"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071612200836600_B1","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1210\/endo-107-5-1286","article-title":"Pulsatile secretion of LH: differential suppression by ovarian steroids.","volume":"107","author":"Goodman","year":"1980","journal-title":"Endocrinology"},{"key":"2020071612200836600_B2","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1146\/annurev.ph.49.030187.002053","article-title":"Central actions of ovarian steroids in the feedback regulation of pulsatile secretion of luteinizing 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hypothalamus.","volume":"143","author":"Foradori","year":"2002","journal-title":"Endocrinology"},{"key":"2020071612200836600_B30","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1016\/j.neuroscience.2009.12.053","article-title":"Forebrain projections of arcuate neurokinin B neurons demonstrated by anterograde tract-tracing and monosodium glutamate lesions in the rat.","volume":"166","author":"Krajewski","year":"2010","journal-title":"Neuroscience"},{"key":"2020071612200836600_B31","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1210\/en.2010-0066","article-title":"Expression of Fos and in vivo median eminence release of LHRH identifies an active role for preoptic area kisspeptin neurons in synchronized surges of LH and LHRH in the ewe.","volume":"152","author":"Hoffman","year":"2011","journal-title":"Endocrinology"},{"key":"2020071612200836600_B32","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0739-7240(97)00059-3","article-title":"Characterization 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