{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T23:59:21Z","timestamp":1791071961057,"version":"4.1.0"},"reference-count":48,"publisher":"The Endocrine Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Polycystic ovarian syndrome (PCOS) is a heterogeneous syndrome associated with follicle growth arrest, minimal granulosa cell proliferation, dysregulated sex hormone profile, hyperthecosis, and insulin resistance. Using a 5\u03b1-dihydrotestosterone (DHT)-induced rat model that recapitulates the reproductive and metabolic phenotypes of human PCOS, we have examined the steroidogenic capability of granulosa cells from DHT-treated rats. Gene expression of several key steroidogenic enzymes including p450 side-chain cleavage enzyme (p450scc), aromatase, steroidogenic acute regulatory protein, hydroxysteroid dehydrogenase-17\u03b2, and hydroxysteroid dehydrogenase-3\u03b2 were markedly lower in DHT-treated rats than the controls, although the responsiveness of their granulosa cells to FSH was higher. Expression of the adipokine chemerin and its receptor, chemokine receptor-like 1, was evident in control and DHT-treated rats, with significantly higher ovarian mRNA abundances and protein contents of chemerin and its receptor. Recombinant chemerin decreases basal estradiol secretion in granulosa cells from DHT-treated rats. When the inhibitory role of chemerin on steroidogenesis was further examined in vitro, chemerin suppressed FSH-induced progesterone and estradiol secretion in cultured preantral follicles and granulosa cells. Chemerin also inhibits FSH-induced aromatase and p450scc expression in granulosa cells. Overexpression of nuclear receptors NR5a1 and NR5a2 promotes p450scc and aromatase expression, respectively, which is suppressed by chemerin. These findings suggest that chemerin is a novel negative regulator of FSH-induced follicular steroidogenesis and may contribute to the pathogenesis of PCOS.<\/jats:p>","DOI":"10.1210\/en.2012-1424","type":"journal-article","created":{"date-parts":[[2012,9,5]],"date-time":"2012-09-05T00:52:36Z","timestamp":1346806356000},"page":"5600-5611","source":"Crossref","is-referenced-by-count":110,"title":["Chemerin, a Novel Regulator of Follicular Steroidogenesis and Its Potential Involvement in Polycystic Ovarian Syndrome"],"prefix":"10.1210","volume":"153","author":[{"given":"Qi","family":"Wang","sequence":"first","affiliation":[{"name":"Departments of Cellular and Molecular Medicine and Obstetrics and Gynecology (Q.W., J.Y.K., A.L., B.K.T.), University of Ottawa, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9"},{"name":"Chronic Disease Program (Q.W., J.Y.K., K.X., A.L., B.K.T.), Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Young","family":"Kim","sequence":"first","affiliation":[{"name":"Departments of Cellular and Molecular Medicine and Obstetrics and Gynecology (Q.W., J.Y.K., A.L., B.K.T.), University of Ottawa, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9"},{"name":"Chronic Disease Program (Q.W., J.Y.K., K.X., A.L., B.K.T.), Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Xue","sequence":"first","affiliation":[{"name":"Chronic Disease Program (Q.W., J.Y.K., K.X., A.L., B.K.T.), Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9"},{"name":"State Key Laboratory in Reproductive Medicine (K.X., J.L.), Clinical Reproductive Medicine Centre, Nanjing Medical University, Nanjing 210008, Peoples' Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-yin","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory in Reproductive Medicine (K.X., J.L.), Clinical Reproductive Medicine Centre, Nanjing Medical University, Nanjing 210008, Peoples' Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur","family":"Leader","sequence":"first","affiliation":[{"name":"Departments of Cellular and Molecular Medicine and Obstetrics and Gynecology (Q.W., J.Y.K., A.L., B.K.T.), University of Ottawa, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9"},{"name":"Chronic Disease Program (Q.W., J.Y.K., K.X., A.L., B.K.T.), Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9"},{"name":"Ottawa Fertility Centre (A.L.), Ottawa, Ontario, Canada K2C 3V4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin K.","family":"Tsang","sequence":"first","affiliation":[{"name":"Departments of Cellular and Molecular Medicine and Obstetrics and Gynecology (Q.W., J.Y.K., A.L., B.K.T.), University of Ottawa, Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9"},{"name":"Chronic Disease Program (Q.W., J.Y.K., K.X., A.L., B.K.T.), Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9"},{"name":"World Class University Biomodulation Major (B.K.T.), Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071612132998000_B1","first-page":"200","article-title":"Initial and cyclic recruitment of ovarian follicles.","volume":"21","author":"McGee","year":"2000","journal-title":"Endocr 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