{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T15:41:12Z","timestamp":1768318872249,"version":"3.49.0"},"reference-count":64,"publisher":"The Endocrine Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,1,1]]},"abstract":"<jats:p>In this study, we have investigated the expression and function of the transcription factor early growth response factor 2 (Egr2)\/Krox20 in the developing anterior pituitary. Egr2 is initially expressed in all differentiating hormonal cells types, but its expression is mostly restricted to the somatotroph lineage after birth. Egr2 knockout results in anterior pituitary hypoplasia. However, the analysis of a conditional mutant demonstrates that this phenotype does not originate from a lack of Egr2 expression in the pituitary. Using an Egr2 allele driving a Cre-activable toxin gene, we performed a genetic ablation of Egr2-positive cells in the pituitary. During the postnatal period, this ablation leads to specific and progressive depletion of the somatotroph population, creating a novel model of early-onset isolated GH deficiency (GHD). Mutant animals were subjected to a complete metabolic analysis, revealing atypical and expected features. Consistent with an adult-onset isolated GHD model, mutant animals are hypoglycemic and display increased insulin sensitivity and glucose clearance. This latter phenotype is in contrast to the glucose intolerance observed in another early-onset GHD model. Surprisingly, increased insulin sensitivity is not accompanied by a modified balance between fat and lean tissues, but by reduced metabolic adaptability between glucose and lipid oxidation conditions. This suggests that the relationship between these metabolic features and insulin sensitivity should be reconsidered. In conclusion, our mutant may be a valuable genetic model with which to study the effects of long-term GH deficiency, in conditions of normal pancreatic function and unaffected balance between fat and glucose metabolism.<\/jats:p>","DOI":"10.1210\/en.2012-1792","type":"journal-article","created":{"date-parts":[[2012,11,14]],"date-time":"2012-11-14T01:59:00Z","timestamp":1352858340000},"page":"270-282","source":"Crossref","is-referenced-by-count":7,"title":["Ablation of Egr2-Positive Cells in Male Mouse Anterior Pituitary Leads to Atypical Isolated GH Deficiency"],"prefix":"10.1210","volume":"154","author":[{"given":"Yassine Xavier","family":"Bouchoucha","sequence":"first","affiliation":[{"name":"Ecole Normale Sup\u00e9rieure (Y.X.B., P.C., P.G.-H.), Institut de Biologie de l\u2019Ecole Normale Sup\u00e9rieure, 75230 Paris cedex 05, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Y.X.B., P.C., P.G.-H.), U1024, 75230 Paris cedex 05, France"},{"name":"Centre National de la Recherche Scientifique (Y.X.B., P.C., P.G.-H.), Unite Mixte de la Recherche 8197, 75230 Paris cedex 05, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Charnay","sequence":"first","affiliation":[{"name":"Ecole Normale Sup\u00e9rieure (Y.X.B., P.C., P.G.-H.), Institut de Biologie de l\u2019Ecole Normale Sup\u00e9rieure, 75230 Paris cedex 05, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Y.X.B., P.C., P.G.-H.), U1024, 75230 Paris cedex 05, France"},{"name":"Centre National de la Recherche Scientifique (Y.X.B., P.C., P.G.-H.), Unite Mixte de la Recherche 8197, 75230 Paris cedex 05, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascale","family":"Gilardi-Hebenstreit","sequence":"first","affiliation":[{"name":"Ecole Normale Sup\u00e9rieure (Y.X.B., P.C., P.G.-H.), Institut de Biologie de l\u2019Ecole Normale Sup\u00e9rieure, 75230 Paris cedex 05, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (Y.X.B., P.C., P.G.-H.), U1024, 75230 Paris cedex 05, France"},{"name":"Centre National de la Recherche Scientifique (Y.X.B., P.C., P.G.-H.), Unite Mixte de la Recherche 8197, 75230 Paris cedex 05, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"key":"2020071612280631400_B1","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1128\/MCB.01885-08","article-title":"Distinct developmental roles of cell cycle inhibitors p57Kip2 and p27Kip1 distinguish pituitary progenitor cell cycle exit from cell cycle reentry of differentiated cells.","volume":"29","author":"Bilodeau","year":"2009","journal-title":"Mol Cell Biol"},{"key":"2020071612280631400_B2","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.ydbio.2011.01.010","article-title":"Birthdating studies reshape models for pituitary gland cell specification.","volume":"352","author":"Davis","year":"2011","journal-title":"Dev Biol"},{"key":"2020071612280631400_B3","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1007\/s11154-005-3047-7","article-title":"Early development of the pituitary gland: induction and shaping of Rathke\u2019s pouch.","volume":"6","author":"Rizzoti","year":"2005","journal-title":"Rev Endocr Metab Disord"},{"key":"2020071612280631400_B4","doi-asserted-by":"crossref","first-page":"790","DOI":"10.1210\/er.2009-0008","article-title":"Genetic regulation of pituitary gland development in human and mouse.","volume":"30","author":"Kelberman","year":"2009","journal-title":"Endocr Rev"},{"key":"2020071612280631400_B5","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1152\/physrev.00006.2006","article-title":"Molecular physiology of pituitary development: signaling and transcriptional networks.","volume":"87","author":"Zhu","year":"2007","journal-title":"Physiol Rev"},{"key":"2020071612280631400_B6","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1097\/MED.0b013e3282ffd97d","article-title":"Adult-onset growth hormone deficiency: causes, complications and treatment options.","volume":"15","author":"Mathioudakis","year":"2008","journal-title":"Curr Opin Endocrinol Diabetes Obes"},{"key":"2020071612280631400_B7","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1210\/jcem.83.2.4545","article-title":"A dose-response study of growth hormone (GH) replacement on whole body protein and lipid kinetics in GH-deficient adults.","volume":"83","author":"Lucidi","year":"1998","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071612280631400_B8","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.ghir.2005.06.018","article-title":"Isolated growth hormone (GH) deficiency in adult patients: baseline clinical characteristics and responses to GH replacement in comparison with hypopituitary patients. 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