{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:56:03Z","timestamp":1789520163422,"version":"build-2803163510"},"reference-count":44,"publisher":"The Endocrine Society","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,5,1]]},"abstract":"<jats:p>Endothelin-1 (EDN1) is an important regulator of H+ secretion in the mammalian kidney. EDN1 enhances renal tubule H+-ATPase activity, but the underlying mechanism remains unclear. To further elucidate the role of EDN1 in vertebrates' acid-base regulation, the present study used zebrafish as the model to examine the effects of EDN1 and its receptors on transepithelial H+ secretion. Expression of EDN1 and one of its receptors, EDNRAa, was stimulated in zebrafish acclimated to acidic water. A noninvasive scanning ion-selective electrode technique was used to show that edn1 overexpression enhances H+ secretion in embryonic skin at 3 days post fertilization. EDNRAa loss of function significantly decreased EDN1- and acid-induced H+ secretion. Abrogation of EDN1-enhanced H+ secretion by a vacuolar H+-ATPase inhibitor (bafilomycin A1) suggests that EDN1 exerts its action by regulating the H+-ATPase-mediated H+ secretion. EDN1 does not appear to affect H+ secretion through either altering the abundance of H+-ATPase or affecting the cell differentiation of H+-ATPase-rich ionocytes, because the reduction in secretion upon ednraa knockdown was not accompanied by decreased expression of H+-ATPase or reduced H+-ATPase-rich cell density. These findings provide evidence that EDN1 signaling is involved in acid-base regulation in zebrafish and enhance our understanding of EDN1 regulation of transepithelial H+ secretion in vertebrates.<\/jats:p>","DOI":"10.1210\/en.2013-1775","type":"journal-article","created":{"date-parts":[[2014,1,14]],"date-time":"2014-01-14T10:06:31Z","timestamp":1389693991000},"page":"1728-1737","source":"Crossref","is-referenced-by-count":13,"title":["Endothelin-1 Regulates H+-ATPase-Dependent Transepithelial H+ Secretion in Zebrafish"],"prefix":"10.1210","volume":"155","author":[{"given":"Ying-Jey","family":"Guh","sequence":"first","affiliation":[{"name":"Graduate Institute of Life Sciences (Y.-J.G., P.-P.H.), National Defense Medical Center, Taipei, Taiwan;"},{"name":"Institute of Cellular and Organismic Biology (Y.-J.G., C.-Y.Y., P.-P.H.), Academia Sinica, Taipei, 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