{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,16]],"date-time":"2024-06-16T04:33:23Z","timestamp":1718512403525},"reference-count":29,"publisher":"The Endocrine Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,7,1]]},"abstract":"<jats:p>The hypothalamic-pituitary-adrenal (HPA) axis is a major part of the neuroendocrine system that controls responses to stress, and has an important function in the regulation of various body processes. We previously created a mouse line deficient in the melanocortin 2 receptor (MC2R). MC2R-deficient mice (MC2R\u2212\/\u2212 mice) have high adrenocorticotropic hormone (ACTH) levels because of undetectable corticosterone levels. Increased neuromedin B (NMB) expression was recently reported in the pituitary gland of adrenalectomized mice, a model for acute adrenal insufficiency. To investigate gene expression in the pituitary gland under chronic adrenal deficiency, we examined the pituitary gland of MC2R\u2212\/\u2212 mice, a model of chronic adrenal insufficiency. To understand the molecular background of pituitary cells under chronic adrenal deficiency, we first performed DNA microarray analyses using the pituitary glands of the MC2R\u2212\/\u2212 mice. The DNA microarray analysis and real-time polymerase chain reaction showed that NMB expression was higher in the MC2R\u2212\/\u2212 than in the wild-type (WT) mice. We detected NMB expression in the MC2R\u2212\/\u2212 pituitary corticotrophs by immunohistochemistry using the specific antibodies for ACTH and NMB. In addition, the plasma NMB concentration was significantly higher in the MC2R\u2212\/\u2212 mice than in the WT mice. Subcutaneous implantation of a sustained-release corticosterone pellet decreased the expression of NMB mRNA as well as pituitary proopiomelanocortin mRNA. In isolated anterior pituitary cells, NMB mRNA expression was increased by the administration of corticotropin-releasing hormone (CRH) and was suppressed by dexamethasone treatment. In this study, we first demonstrate NMB expression in corticotrophs and its regulation by CRH and glucocorticoids. Furthermore, corticotrophs seemed to secrete NMB into the systemic circulation.<\/jats:p>","DOI":"10.1210\/en.2013-2077","type":"journal-article","created":{"date-parts":[[2014,4,17]],"date-time":"2014-04-17T16:04:59Z","timestamp":1397750699000},"page":"2492-2499","source":"Crossref","is-referenced-by-count":7,"title":["Expression and Regulation of Neuromedin B in Pituitary Corticotrophs of Male Melanocortin 2 Receptor-Deficient Mice"],"prefix":"10.1210","volume":"155","author":[{"given":"Hiraku","family":"Kameda","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Endocrinology and Nephrology (H.K., H.M., A.N., T.K., T.A.), Hokkaido University Graduate School of Medicine, N 15, W 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Miyoshi","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Endocrinology and Nephrology (H.K., H.M., A.N., T.K., T.A.), Hokkaido University Graduate School of Medicine, N 15, W 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chikara","family":"Shimizu","sequence":"first","affiliation":[{"name":"Division of Laboratory and Transfusion Medicine (C.S.), Hokkaido University Hospital, N 14, W 5, Kita-ku, Sapporo, Hokkaido 060-8648 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"So","family":"Nagai","sequence":"first","affiliation":[{"name":"Department of Diabetes and Endocrinology (S.N.), Sapporo Medical Center; NTT EC, S 1, W 15, Chuoh-Ku, Sapporo, Hokkaido 060-0061, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akinobu","family":"Nakamura","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Endocrinology and Nephrology (H.K., H.M., A.N., T.K., T.A.), Hokkaido University Graduate School of Medicine, N 15, W 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuma","family":"Kondo","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Endocrinology and Nephrology (H.K., H.M., A.N., T.K., T.A.), Hokkaido University Graduate School of Medicine, N 15, W 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dai","family":"Chida","sequence":"first","affiliation":[{"name":"Faculty of Medicine (D.C.), Saitama Medical University, 38 Morohongo, Moroyama-machi, Iruma-gun, Saitama 350-0495, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuya","family":"Atsumi","sequence":"first","affiliation":[{"name":"Division of Rheumatology, Endocrinology and Nephrology (H.K., H.M., A.N., T.K., T.A.), Hokkaido University Graduate School of Medicine, N 15, W 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"key":"2020071612204223300_B1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1080\/07435808409036499","article-title":"Control of adrenocortical growth in vivo","volume":"10","author":"Dallman","year":"1984","journal-title":"Endocr Res"},{"key":"2020071612204223300_B2","doi-asserted-by":"crossref","first-page":"18205","DOI":"10.1073\/pnas.0706953104","article-title":"Melanocortin 2 receptor is required for adrenal gland development, steroidogenesis, and neonatal gluconeogenesis","volume":"104","author":"Chida","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2020071612204223300_B3","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.mce.2008.10.027","article-title":"Characterization of mice deficient in melanocortin 2 receptor on a B6\/Balbc mix background","volume":"300","author":"Chida","year":"2009","journal-title":"Mol Cell Endocrinol"},{"key":"2020071612204223300_B4","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1016\/0006-291X(84)91046-5","article-title":"Neuromedin B is a major bombesin-like peptide in rat brain: regional distribution of neuromedin B and neuromedin C in rat brain, pituitary and spinal cord","volume":"124","author":"Minamino","year":"1984","journal-title":"Biochem Biophys Res Commun"},{"key":"2020071612204223300_B5","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0301-0082(00)00004-6","article-title":"Neuromedin B","volume":"62","author":"Ohki-Hamazaki","year":"2000","journal-title":"Prog Neurobiol"},{"key":"2020071612204223300_B6","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/0006-291X(84)91611-5","article-title":"Neuromedin C: a bombesin-like peptide identified in porcine spinal cord","volume":"119","author":"Minamino","year":"1984","journal-title":"Biochem Biophys Res Commun"},{"key":"2020071612204223300_B7","doi-asserted-by":"crossref","first-page":"37","DOI":"10.2170\/jjphysiol.45.37","article-title":"Effects of neuromedins and related peptides on the body temperature of rats","volume":"45","author":"Itoh","year":"1995","journal-title":"Jpn J Physiol"},{"key":"2020071612204223300_B8","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0167-0115(93)90417-7","article-title":"Effects of central neuromedin B and related peptides on blood glucose","volume":"47","author":"Plamondon","year":"1993","journal-title":"Regul Pept"},{"key":"2020071612204223300_B9","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1159\/000199318","article-title":"Effects of decapeptide of mammalian bombesin and neuromedin B on pancreatic exocrine secretion in the rat","volume":"34","author":"Namba","year":"1986","journal-title":"Digestion"},{"key":"2020071612204223300_B10","first-page":"G491","article-title":"Effects of neuromedin B and neuromedin C on exocrine and endocrine rat pancreas","volume":"252","author":"Otsuki","year":"1987","journal-title":"Am J Physiol"},{"key":"2020071612204223300_B11","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1002\/(SICI)1097-0045(20000301)42:4<295::AID-PROS7>3.0.CO;2-B","article-title":"Presence of receptors for bombesin\/gastrin-releasing peptide and mRNA for three receptor subtypes in human prostate cancers","volume":"42","author":"Sun","year":"2000","journal-title":"Prostate"},{"key":"2020071612204223300_B12","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1006\/pupt.1999.0210","article-title":"Evidence for autocrine actions of neuromedin B and gastrin-releasing peptide in non-small cell lung cancer","volume":"12","author":"Siegfried","year":"1999","journal-title":"Pulm Pharmacol Ther"},{"key":"2020071612204223300_B13","doi-asserted-by":"crossref","first-page":"G718","DOI":"10.1152\/ajpgi.00156.2004","article-title":"Neuromedin B and its receptor are mitogens in both normal and malignant epithelial cells lining the colon","volume":"288","author":"Matusiak","year":"2005","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"2020071612204223300_B14","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1530\/JOE-12-0488","article-title":"Autocrine effects of neuromedin B stimulate the proliferation of rat primary osteoblasts","volume":"217","author":"Saito","year":"2013","journal-title":"J Endocrinol"},{"key":"2020071612204223300_B15","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/0196-9781(85)90383-3","article-title":"Presence of neuromedin B-like immunoreactivity in the brain and gut of rat and guinea-pig","volume":"3","author":"Namba","year":"1985","journal-title":"Peptides"},{"key":"2020071612204223300_B16","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1677\/jme.1.01892","article-title":"Disruption of neuromedin B receptor gene results in dysregulation of the pituitary-thyroid axis","volume":"36","author":"Oliveira","year":"2006","journal-title":"J Mol Endocrinol"},{"key":"2020071612204223300_B17","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0940-2993(11)80279-4","article-title":"Investigations on the acute effects of neuropeptides on the pituitary-adrenocortical function in normal and cold-stressed rats. I. Bombesin and neuromedin B","volume":"47","author":"Malendowicz","year":"1995","journal-title":"Exp Toxicol Pathol"},{"key":"2020071612204223300_B18","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1038\/oby.2008.484","article-title":"Regulation of pituitary gene expression by adrenalectomy","volume":"17","author":"Nishida","year":"2009","journal-title":"Obesity"},{"key":"2020071612204223300_B19","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.1210\/en.2005-0751","article-title":"Steroid receptor coactivator-1-deficient mice exhibit altered hypothalamic-pituitary-adrenal axis function","volume":"147","author":"Winnay","year":"2006","journal-title":"Endocrinology"},{"key":"2020071612204223300_B20","doi-asserted-by":"crossref","first-page":"6956","DOI":"10.1523\/JNEUROSCI.2584-06.2007","article-title":"Serotonin activates the hypothalamic-pituitary-adrenal axis via serotonin 2C receptor stimulation","volume":"27","author":"Heisler","year":"2007","journal-title":"J Neurosci"},{"key":"2020071612204223300_B21","doi-asserted-by":"crossref","first-page":"e19559","DOI":"10.1371\/journal.pone.0019559","article-title":"MCP-1 upregulates amylin expression in murine pancreatic beta cells through ERK\/JNK-AP1 and NF-kappaB related signaling pathways independent of CCR2","volume":"6","author":"Cai","year":"2011","journal-title":"PloS one"},{"key":"2020071612204223300_B22","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"2020071612204223300_B23","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1677\/joe.0.1570053","article-title":"Stimulatory effect of insulin-like growth factor I on proliferation of mouse pituitary cells in serum-free culture","volume":"157","author":"Oomizu","year":"1998","journal-title":"J Endocrinol"},{"key":"2020071612204223300_B24","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1210\/endo-122-1-270","article-title":"Localization of 7B2, neuromedin B, and neuromedin U in specific cell types of rat, mouse, and human pituitary, in rat hypothalamus, and in 30 human pituitary and extrapituitary tumors","volume":"122","author":"Steel","year":"1988","journal-title":"Endocrinology"},{"key":"2020071612204223300_B25","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1042\/bj2860641","article-title":"Activation of neuromedin B-preferring bombesin receptors on rat glioblastoma C-6 cells increases cellular Ca2+ and phosphoinositides","volume":"286","author":"Wang","year":"1992","journal-title":"Biochem J"},{"key":"2020071612204223300_B26","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1016\/0896-6273(91)90250-4","article-title":"cDNA cloning, characterization, and brain region-specific expression of a neuromedin-B-preferring bombesin receptor","volume":"6","author":"Wada","year":"1991","journal-title":"Neuron"},{"key":"2020071612204223300_B27","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0006-8993(97)00380-6","article-title":"Cloning and expression of the neuromedin B receptor and the third subtype of bombesin receptor genes in the mouse","volume":"762","author":"Ohki-Hamazaki","year":"1997","journal-title":"Brain Res"},{"key":"2020071612204223300_B28","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.nbd.2009.07.030","article-title":"Structure and function of the blood-brain barrier","volume":"37","author":"Abbott","year":"2010","journal-title":"Neurobiol Dis"},{"key":"2020071612204223300_B29","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1054\/npep.1999.0054","article-title":"Role of bombesin-related peptides in the control of food intake","volume":"33","author":"Merali","year":"1999","journal-title":"Neuropeptides"}],"container-title":["Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/endo\/article-pdf\/155\/7\/2492\/10826349\/endo2492.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/endo\/article-pdf\/155\/7\/2492\/10826349\/endo2492.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T19:56:10Z","timestamp":1594929370000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/endo\/article\/155\/7\/2492\/2423446"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,1]]},"references-count":29,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,7,1]]}},"URL":"https:\/\/doi.org\/10.1210\/en.2013-2077","relation":{},"ISSN":["0013-7227","1945-7170"],"issn-type":[{"value":"0013-7227","type":"print"},{"value":"1945-7170","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,1]]}}}