{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T06:52:33Z","timestamp":1777531953943,"version":"3.51.4"},"reference-count":38,"publisher":"The Endocrine Society","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Aromatase, encoded by Cyp19a1, is expressed in the pituitary of vertebrates; however, its physiological relevance remains poorly defined. In teleosts, the duplicated cyp19a1b is preferentially expressed in the pituitary where LH and FSH are synthesized in distinct gonadotropes. Our present study demonstrated that Cyp19a1b is colocalized with Lhb, but not Fshb, during vitellogenesis in female ricefield eels. The immunoreactive levels of Cyp19a1b and Lhb, as well as their colocalization frequency, increased during vitellogenesis toward maturation. The expression of lhb but not fshb in the pituitary fragments of female ricefield eels was induced by both estradiol (E2) and testosterone (T). In agreement, the promoter of lhb but not fshb was activated by both E2 and T. T is more potent than E2 in inducing lhb expression, whereas E2 is much more effective in activating the lhb promoter. T-induced lhb expression in the pituitary fragments was abolished by the estrogen receptor (Esr) antagonist fulvestrant and suppressed by the aromatase inhibitor letrozole, suggesting that the effect of T on lhb expression at the pituitary is largely mediated by E2. Furthermore, Lhb was shown to colocalize with Esr1 but not Esr2a. Taken together, results of the present study suggest that Cyp19a1b in LH cells may greatly upregulate lhb expression during vitellogenesis, possibly via E2 and Esr1 in an intracrine manner. The absence of Cyp19a1b in FSH cells and the insensitivity of fshb to sex steroids may contribute to the differential expression of lhb and fshb in ricefield eels and possibly other vertebrates as well.<\/jats:p>","DOI":"10.1210\/en.2014-1069","type":"journal-article","created":{"date-parts":[[2014,8,8]],"date-time":"2014-08-08T17:21:22Z","timestamp":1407518482000},"page":"4531-4541","source":"Crossref","is-referenced-by-count":23,"title":["Aromatase (Cyp19a1b) in the Pituitary Is Dynamically Involved in the Upregulation of lhb But Not fshb in the Vitellogenic Female Ricefield Eel Monopterus albus"],"prefix":"10.1210","volume":"155","author":[{"given":"Shen","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Life Sciences (S.Z., Y.Z., W.C., Y.W., L.Z., W.Z.), Sun Yat-sen University, Guangzhou 510275, People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Zhang","sequence":"first","affiliation":[{"name":"School of 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