{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T14:27:12Z","timestamp":1790000832392,"version":"4.0.1"},"reference-count":42,"publisher":"The Endocrine Society","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,8,1]]},"abstract":"<jats:p>The large-conductance Ca2+-activated K+ (BK) channel plays an essential role in maintaining uterine quiescence during pregnancy. Growing evidence has shown a link between the BK channel and bacterial lipopolysaccharide (LPS)-induced nuclear factor-\u03baB (NF-\u03baB) activation in macrophages. In the uterus, NF-\u03baB activation plays an important role in inflammatory processes that lead to parturition. Our objective was to determine whether the BK channel regulates uterine contraction, in part, by modulating NF-\u03baB translocation into the nucleus. We compared the effects of BK channel modulation to those of LPS on NF-\u03baB nuclear translocation and contraction in an immortalized human myometrial cell line (human telomerase reverse transcriptase [hTERT]) and uterine myocytes. Our results showed that BK channel inhibitors paxilline and penitrem A induced translocation of NF-\u03baB into the nucleus in both hTERT cells and uterine myocytes to a similar extent as LPS treatment, and LPS and paxilline similarly reduced BK channel currents. Conversely, neither BK channel openers nor blockade of the small conductance Ca2+-activated K+ channel protein 3 had an effect on NF-\u03baB translocation. Additionally, collagen-based assays showed that paxilline induced contraction of hTERT cells and uterine myocytes. This was dependent upon cyclooxygenase-2 activity. Moreover, paxilline-induced contractility and increased cyclooxygenase-2 expression both depended on availability of free NF-\u03baB. This study suggests that BK channels regulate myometrial contraction, in part, by modulating nuclear translocation of NF-\u03baB.<\/jats:p>","DOI":"10.1210\/en.2014-1152","type":"journal-article","created":{"date-parts":[[2014,6,10]],"date-time":"2014-06-10T15:48:24Z","timestamp":1402415304000},"page":"3112-3122","source":"Crossref","is-referenced-by-count":18,"title":["BK Channels Regulate Myometrial Contraction by Modulating Nuclear Translocation of NF-\u03baB"],"prefix":"10.1210","volume":"155","author":[{"given":"Youe","family":"Li","sequence":"first","affiliation":[{"name":"Center for Women's Reproductive Sciences Research, Department of Obstetrics and Gynecology, Basic Science Division, Washington University in St Louis, St Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n A.","family":"Lorca","sequence":"first","affiliation":[{"name":"Center for Women's Reproductive Sciences Research, Department of Obstetrics and Gynecology, Basic 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