{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:15:50Z","timestamp":1792653350336,"version":"4.5.0"},"reference-count":27,"publisher":"The Endocrine Society","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,7,1]]},"abstract":"<jats:p>Transgenic animal models are valuable for studying gene function in various tissue compartments. Mice with conditional deletion of genes in the uterus using the Cre-loxP system serve as powerful tools to study uterine biology. The uterus is comprised of 3 major tissue types: myometrium, stroma, and epithelium. Proliferation and differentiation in each uterine cell type are differentially regulated by ovarian hormones, resulting in spatiotemporal control of gene expression. Therefore, examining gene function in each uterine tissue type will provide more meaningful information regarding uterine biology during pregnancy and disease states. Although currently available Cre mouse lines have been very useful in exploring functions of specific genes in uterine biology, overlapping expression of these Cre lines in more than 1 tissue type and in other reproductive organs sometimes makes interpretation of results difficult. In this article, we report the generation of a new iCre knock-in mouse line, in which iCre is expressed from endogenous lactoferrin (Ltf) promoter. Ltf-iCre mice primarily direct recombination in the uterine epithelium in adult females and in immature females after estrogen treatment. These mice will allow for specific interrogation of gene function in the mature uterine epithelium, providing a helpful tool to uncover important aspects of uterine biology.<\/jats:p>","DOI":"10.1210\/en.2014-1265","type":"journal-article","created":{"date-parts":[[2014,5,13]],"date-time":"2014-05-13T13:56:33Z","timestamp":1399989393000},"page":"2718-2724","source":"Crossref","is-referenced-by-count":111,"title":["Lactoferrin-iCre: A New Mouse Line to Study Uterine Epithelial Gene Function"],"prefix":"10.1210","volume":"155","author":[{"given":"Takiko","family":"Daikoku","sequence":"first","affiliation":[{"name":"Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, Ohio 45229\u20133039"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuya","family":"Ogawa","sequence":"first","affiliation":[{"name":"Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, Ohio 45229\u20133039"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jumpei","family":"Terakawa","sequence":"first","affiliation":[{"name":"Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, Ohio 45229\u20133039"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiyo","family":"Ogawa","sequence":"first","affiliation":[{"name":"Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, Ohio 45229\u20133039"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony","family":"DeFalco","sequence":"first","affiliation":[{"name":"Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, Ohio 45229\u20133039"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudhansu K.","family":"Dey","sequence":"first","affiliation":[{"name":"Division of Reproductive Sciences, Perinatal Institute, Cincinnati Children\u2019s Hospital Medical Center, Cincinnati, Ohio 45229\u20133039"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"issue":"3","key":"2020071612330136000_B1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/nrg1808","article-title":"Roadmap to embryo implantation: clues from mouse models","volume":"7","author":"Wang","year":"2006","journal-title":"Nat Rev Genet"},{"issue":"12","key":"2020071612330136000_B2","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.1038\/nm.3012","article-title":"Mechanisms of implantation: strategies for successful pregnancy","volume":"18","author":"Cha","year":"2012","journal-title":"Nat Med"},{"issue":"2","key":"2020071612330136000_B3","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1002\/gene.20098","article-title":"Cre-mediated recombination in cell lineages that express the progesterone receptor","volume":"41","author":"Soyal","year":"2005","journal-title":"Genesis"},{"issue":"7","key":"2020071612330136000_B4","doi-asserted-by":"crossref","first-page":"1154","DOI":"10.1002\/mrd.20858","article-title":"A mesenchymal perspective of M\u00fcllerian duct differentiation and regression in Amhr2-lacZ mice","volume":"75","author":"Arango","year":"2008","journal-title":"Mol Reprod Dev"},{"issue":"7","key":"2020071612330136000_B5","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1093\/molehr\/gat016","article-title":"Conditional deletion of Tsc1 in the female reproductive tract impedes normal oviductal and uterine function by enhancing mTORC1 signaling in mice","volume":"19","author":"Daikoku","year":"2013","journal-title":"Mol Hum Reprod"},{"issue":"2","key":"2020071612330136000_B6","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1016\/j.ygyno.2011.04.022","article-title":"Cell-specific conditional deletion of Pten in the uterus results in differential phenotypes","volume":"122","author":"Daikoku","year":"2011","journal-title":"Gynecol Oncol"},{"issue":"3\u20134","key":"2020071612330136000_B7","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1242\/dmm.004440","article-title":"Lkb1 inactivation is sufficient to drive endometrial cancers that are aggressive yet highly responsive to mTOR inhibitor monotherapy","volume":"3","author":"Contreras","year":"2010","journal-title":"Dis Model Mech"},{"issue":"4","key":"2020071612330136000_B8","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1073\/pnas.1118411109","article-title":"Kruppel-like factor 5 (KLF5) is critical for conferring uterine receptivity to implantation","volume":"109","author":"Sun","year":"2012","journal-title":"Proc Natl Acad Sci USA"},{"issue":"5","key":"2020071612330136000_B9","doi-asserted-by":"crossref","first-page":"2380","DOI":"10.1096\/fj.13-248344","article-title":"Ovarian LGR5 is critical for successful pregnancy","volume":"28","author":"Sun","year":"2014","journal-title":"FASEB J"},{"issue":"2","key":"2020071612330136000_B10","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1095\/biolreprod.111.091769","article-title":"Postnatal deletion of Wnt7a inhibits uterine gland morphogenesis and compromises adult fertility in mice","volume":"85","author":"Dunlap","year":"2011","journal-title":"Biol Reprod"},{"issue":"9","key":"2020071612330136000_B11","doi-asserted-by":"crossref","first-page":"1442","DOI":"10.1210\/me.2012-1126","article-title":"CTNNB1 in mesenchyme regulates epithelial cell differentiation during M\u00fcllerian duct and postnatal uterine development","volume":"27","author":"Stewart","year":"2013","journal-title":"Mol Endocrinol"},{"issue":"4","key":"2020071612330136000_B12","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1096\/fj.10-175349","article-title":"WNT4 is a key regulator of normal postnatal uterine development and progesterone signaling during embryo implantation and decidualization in the mouse","volume":"25","author":"Franco","year":"2011","journal-title":"FASEB J"},{"issue":"5","key":"2020071612330136000_B13","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1095\/biolreprod.101.002089","article-title":"Differential expression and estrogen response of lactoferrin gene in the female reproductive tract of mouse, rat, and hamster","volume":"67","author":"Teng","year":"2002","journal-title":"Biol Reprod"},{"issue":"6","key":"2020071612330136000_B14","doi-asserted-by":"crossref","first-page":"2905","DOI":"10.1210\/endo.139.6.6051","article-title":"Differential spatiotemporal regulation of lactoferrin and progesterone receptor genes in the mouse uterus by primary estrogen, catechol estrogen, and xenoestrogen","volume":"139","author":"Das","year":"1998","journal-title":"Endocrinology"},{"issue":"5","key":"2020071612330136000_B15","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1095\/biolreprod47.5.903","article-title":"Fluctuations of lactoferrin protein and messenger-ribonucleic-acid in the reproductive-tract of the mouse during the estrous cycle","volume":"47","author":"Newbold","year":"1992","journal-title":"Biol Reprod"},{"issue":"1","key":"2020071612330136000_B16","first-page":"101","article-title":"Lactoferrin in the mouse uterus: analyses of the preimplantation period and regulation by ovarian steroids","volume":"6","author":"McMaster","year":"1992","journal-title":"Mol Endocrinol"},{"issue":"6","key":"2020071612330136000_B17","doi-asserted-by":"crossref","first-page":"3459","DOI":"10.1210\/endo.130.6.1375903","article-title":"Expression of transforming growth factor-\u03b2 isoforms (\u03b2 2 and \u03b2 3) in the mouse uterus: analysis of the periimplantation period and effects of ovarian steroids","volume":"130","author":"Das","year":"1992","journal-title":"Endocrinology"},{"issue":"1","key":"2020071612330136000_B18","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1128\/MCB.23.1.178-185.2003","article-title":"Iron status in mice carrying a targeted disruption of lactoferrin","volume":"23","author":"Ward","year":"2003","journal-title":"Mol Cell Biol"},{"issue":"1","key":"2020071612330136000_B19","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1006\/geno.2000.6451","article-title":"A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA","volume":"73","author":"Lee","year":"2001","journal-title":"Genomics"},{"issue":"5881","key":"2020071612330136000_B20","doi-asserted-by":"crossref","first-page":"1336","DOI":"10.1126\/science.1157676","article-title":"Intersection of the RNA interference and X-inactivation pathways","volume":"320","author":"Ogawa","year":"2008","journal-title":"Science"},{"issue":"12","key":"2020071612330136000_B21","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1002\/dvg.20356","article-title":"Foxb1-driven Cre expression in somites and the neuroepithelium of diencephalon, brainstem, and spinal cord","volume":"45","author":"Zhao","year":"2007","journal-title":"Genesis"},{"issue":"11","key":"2020071612330136000_B22","doi-asserted-by":"crossref","first-page":"4455","DOI":"10.1073\/pnas.0609277104","article-title":"Developmental and adult phenotyping directly from mutant embryonic stem cells","volume":"104","author":"George","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"issue":"11","key":"2020071612330136000_B23","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1210\/mend.15.11.0734","article-title":"Adult tissue angiogenesis: evidence for negative regulation by estrogen in the uterus","volume":"15","author":"Ma","year":"2001","journal-title":"Mol Endocrinol"},{"issue":"1","key":"2020071612330136000_B24","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1002\/gene.10023","article-title":"Codon-improved Cre recombinase (iCre) expression in the mouse","volume":"32","author":"Shimshek","year":"2002","journal-title":"Genesis"},{"issue":"4","key":"2020071612330136000_B25","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1210\/endo.140.4.6671","article-title":"Restricted spatiotemporal expression of lactoferrin during murine embryonic development","volume":"140","author":"Ward","year":"1999","journal-title":"Endocrinology"},{"issue":"4","key":"2020071612330136000_B26","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.2353\/ajpath.2008.061145","article-title":"Stimulus-dependent impairment of the neutrophil oxidative burst response in lactoferrin-deficient mice","volume":"172","author":"Ward","year":"2008","journal-title":"Am J Pathol"},{"issue":"4","key":"2020071612330136000_B27","doi-asserted-by":"crossref","first-page":"301.e1","DOI":"10.1016\/j.ijantimicag.2008.07.020","article-title":"Lactoferrin: structure, function and applications","volume":"33","author":"Gonz\u00e1lez-Ch\u00e1vez","year":"2009","journal-title":"Int J Antimicrob Agents"}],"container-title":["Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/endo\/article-pdf\/155\/7\/2718\/10826579\/endo2718.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/endo\/article-pdf\/155\/7\/2718\/10826579\/endo2718.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T16:38:32Z","timestamp":1594917512000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/endo\/article\/155\/7\/2718\/2423913"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,1]]},"references-count":27,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,7,1]]}},"URL":"https:\/\/doi.org\/10.1210\/en.2014-1265","relation":{},"ISSN":["0013-7227","1945-7170"],"issn-type":[{"value":"0013-7227","type":"print"},{"value":"1945-7170","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,1]]}}}