{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T08:46:51Z","timestamp":1767862011488,"version":"3.49.0"},"reference-count":28,"publisher":"The Endocrine Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,2,1]]},"abstract":"<jats:p>The purpose of the study was to investigate urocortin (Ucn)2 involvement in placental and myometrial inflammatory pathways associated with parturition by evaluating: 1) Ucn2 and its receptor, CRH-receptor type 2 (CRH-R2), expression in laboring\/nonlaboring human gestational tissues and in mouse utero-placental tissues approaching delivery; and 2) Ucn2 effect on myometrial contractility and on the expression of inflammatory mediators (prostaglandin F2\u03b1 receptor and cytokines) and regulation of Ucn2 by TNF-\u03b1 in cultured myometrial cell line. Placenta (n = 16), fetal membranes (n = 16), and myometrium (n = 22) were obtained from healthy pregnant women delivering at term by vaginal\/elective caesarean delivery and from timed-pregnant mice on days 16\u201319. Expression of Ucn2\/CRH-R2 in human\/mouse tissues and inflammatory mediators in myometrial cell lines were measured by RT-PCR or ELISA, mouse Ucn2\/CRH-R2 protein localization by immunohistochemistry. Ucn2 but not CRH-R2 was up-regulated (P &amp;lt; .05) in all human tissues in labor (compared with before labor) and increased significantly (P &amp;lt; .01) in mouse placenta approaching delivery. Ucn2 was up-regulated by TNF-\u03b1 via nuclear factor-\u03baB (NF-kB) in myometrium cell lines (P &amp;lt; .05 or P &amp;lt; .01 on the basis of treatment doses) and increased proinflammatory mediators and prostaglandin F (PGF2\u03b1) receptor expression (P &amp;lt; .05) via CRH-R2, without a direct effect on contractility. Placental and myometrial Ucn2 may play a role in the endocrine-inflammatory processes of parturition, representing a potential target for treating inflammation-induced obstetric complications.<\/jats:p>","DOI":"10.1210\/en.2014-1432","type":"journal-article","created":{"date-parts":[[2014,11,26]],"date-time":"2014-11-26T19:26:48Z","timestamp":1417030008000},"page":"670-679","source":"Crossref","is-referenced-by-count":16,"title":["Urocortin 2 Role in Placental and Myometrial Inflammatory Mechanisms at Parturition"],"prefix":"10.1210","volume":"156","author":[{"given":"Chiara","family":"Voltolini","sequence":"first","affiliation":[{"name":"Department of Molecular and Developmental Medicine (C.V., R.N., M.T., F.M.S., F.P.), University of Siena, 53100 Siena, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon","family":"Battersby","sequence":"first","affiliation":[{"name":"Medical Research Council Centre for Reproductive Health (S.B., J.E.N.), University of 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