{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T12:12:34Z","timestamp":1754395954656},"reference-count":36,"publisher":"The Endocrine Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) bind to the receptor guanylyl cyclase (GC)-A, leading to diuresis, natriuresis, and blood vessel dilation. In addition, ANP and BNP have various angiogenic properties in ischemic tissue. When breeding mice devoid of GC-A, we noted significant skewing of the Mendelian ratio in the offspring, suggesting embryonic lethality due to knockout of GC-A. Consequently, we here investigated the roles of endogenous ANP and BNP in embryonic neovascularization and organ morphogenesis. Embryos resulting from GC-A\u2212\/\u2212 \u00d7 GC-A+\/\u2212 crosses developed hydrops fetalis (HF) beginning at embryonic day (E)14.5. All embryos with HF had the genotype GC-A\u2212\/\u2212. At E17.5, 33.3% (12 of 36) of GC-A\u2212\/\u2212 embryos had HF, and all GC-A\u2212\/\u2212 embryos with HF were dead. Beginning at E16.0, HF-GC-A\u2212\/\u2212 embryos demonstrated poorly developed superficial vascular vessels and sc hemorrhage, the fetal side of the placenta appeared ischemic, and vitelline vessels on the yolk sac were poorly developed. Furthermore, HF-GC-A\u2212\/\u2212 embryos also showed abnormal constriction of umbilical cord vascular vessels, few cardiac trabeculae and a thin compact zone, hepatic hemorrhage, and poor bone development. Electron microscopy of E16.5 HF-GC-A\u2212\/\u2212 embryos revealed severe vacuolar degeneration in endothelial cells, and the expected 3-layer structure of the smooth muscle wall of the umbilical artery was indistinct. These data demonstrate the importance of the endogenous ANP\/BNP-GC-A system not only in the neovascularization of ischemic tissues but also in embryonic vascular development and organ morphogenesis.<\/jats:p>","DOI":"10.1210\/en.2015-1344","type":"journal-article","created":{"date-parts":[[2015,10,30]],"date-time":"2015-10-30T17:55:05Z","timestamp":1446227705000},"page":"358-367","source":"Crossref","is-referenced-by-count":6,"title":["Importance of Endogenous Atrial and Brain Natriuretic Peptides in Murine Embryonic Vascular and Organ Development"],"prefix":"10.1210","volume":"157","author":[{"given":"Takeshi","family":"Tokudome","sequence":"first","affiliation":[{"name":"Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Kishimoto","sequence":"first","affiliation":[{"name":"Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Shindo","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayato","family":"Kawakami","sequence":"first","affiliation":[{"name":"Department of Anatomy (H.K.), Kyorin University School of Medicine, Mitaka, Tokyo, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teruhide","family":"Koyama","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Research (T.S., T.K.), Shinshu University Graduate School of Medicine, Shinshu, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kentaro","family":"Otani","sequence":"first","affiliation":[{"name":"Tissue Engineering and Regenerative Medicine (K.O.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohito","family":"Nishimura","sequence":"first","affiliation":[{"name":"Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikiya","family":"Miyazato","sequence":"first","affiliation":[{"name":"Department of Biochemistry (T.T., I.K., H.N., M.M.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Kohno","sequence":"first","affiliation":[{"name":"Department of Cardiorenal and Cerebrovascular Medicine (M.K.), Kagawa University Faculty of Medicine, Kagawa, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuwa","family":"Nakao","sequence":"first","affiliation":[{"name":"Kyoto University Graduate School of Medicine Medical Innovation Center (K.N.), Kyoto, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Kangawa","sequence":"first","affiliation":[{"name":"Director General (K.K.), National Cerebral and Cardiovascular Research Center, Suita, Osaka, 565-8565 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"key":"2020071611590513900_B1","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1139\/y11-022","article-title":"Roles of guanylyl cyclase\u2013a signaling in the cardiovascular system","volume":"89","author":"Saito","year":"2011","journal-title":"Can J Physiol Pharmacol"},{"key":"2020071611590513900_B2","doi-asserted-by":"crossref","first-page":"3404","DOI":"10.1073\/pnas.0538059100","article-title":"Significance and 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