{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:40:20Z","timestamp":1777480820455,"version":"3.51.4"},"reference-count":27,"publisher":"The Endocrine Society","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Dmbx1 is a brain-specific homeodomain transcription factor expressed primarily during embryogenesis, and its systemic disruption (Dmbx1\u2212\/\u2212) in the ICR mouse strain resulted in leanness associated with impaired long-lasting orexigenic effect of agouti-related peptide (AgRP). Because spatial and temporal expression patterns of Dmbx1 change dramatically during embryogenesis, it remains unknown when and where Dmbx1 plays a critical role in energy homeostasis. In the present study, the physiological roles of Dmbx1 were examined by its conditional disruption (Dmbx1loxP\/loxP) in the C57BL\/6 mouse strain. Although Dmbx1 disruption in fetal brain resulted in neonatal lethality, its disruption by synapsin promoter-driven Cre recombinase, which eliminated Dmbx1 expression postnatally, exempted the mice (Syn-Cre;Dmbx1loxP\/loxP mice) from lethality. Syn-Cre;Dmbx1loxP\/loxP mice show mild leanness and impaired long-lasting orexigenic action of AgRP, demonstrating the physiological relevance of Dmbx1 in the adult. Visualization of Dmbx1-expressing neurons in adult brain using the mice harboring tamoxifen-inducible Cre recombinase in the Dmbx1 locus (Dmbx1CreERT2\/+ mice) revealed Dmbx1 expression in small numbers of neurons in restricted regions, including the lateral parabrachial nucleus (LPB). Notably, c-Fos expression in LPB was increased at 48 hours after AgRP administration in Dmbx1loxP\/loxP mice but not in Syn-Cre;Dmbx1loxP\/loxP mice. These c-Fos-positive neurons in LPB did not coincide with neurons expressing Dmbx1 or melanocortin 4 receptor but did coincide with those expressing calcitonin gene-related peptide. Accordingly, Dmbx1 in the adult LPB is required for the long-lasting orexigenic effect of AgRP via the neural circuitry involving calcitonin gene-related peptide neurons.<\/jats:p>","DOI":"10.1210\/en.2015-1560","type":"journal-article","created":{"date-parts":[[2015,10,27]],"date-time":"2015-10-27T17:03:07Z","timestamp":1445965387000},"page":"245-257","source":"Crossref","is-referenced-by-count":6,"title":["Importance of Adult Dmbx1 in Long-Lasting Orexigenic Effect of Agouti-Related Peptide"],"prefix":"10.1210","volume":"157","author":[{"given":"Seiichiro","family":"Hirono","sequence":"first","affiliation":[{"name":"Departments of Medical Physiology (S.H., E.Y.L., S.K., T.M.) 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