{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:22:09Z","timestamp":1792660929178,"version":"4.5.0"},"reference-count":59,"publisher":"The Endocrine Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We recently showed that the mouse pituitary holds regenerative competence. Young-adult GHCre\/iDTR mice, expressing diphtheria toxin (DT) receptor in GH-producing cells, regenerate the GH+ cells, as ablated by 3-day DT treatment (3DT), up to 60% after 5 months. The pituitary's stem cells participate in this restoration process. Here, we characterized this regenerative capacity in relation to age and recovery period and started to search for underlying molecular mechanisms. Extending the recovery period (up to 19 mo) does not result in higher regeneration levels. In addition, the regenerative competence disappears at older age, coinciding with a reduction in pituitary stem cell number and fitness. Surprisingly, prolonging DT treatment of young-adult mice to 10 days (10DT) completely blocks the regeneration, although the stem cell compartment still reacts by promptly expanding, and retains in vitro stem cell functionality. To obtain a first broad view on molecular grounds underlying reparative capacity and\/or failure, the stem cell-clustering side population was analyzed by whole-genome expression analysis. A number of stemness factors and components of embryonic, epithelial-mesenchymal transition, growth factor and Hippo pathways are higher expressed in the stem cell-clustering side population of the regenerating pituitary (after 3DT) when compared with the basal gland and to the nonregenerating pituitary (after 10DT). Together, the regenerative capacity of the pituitary is limited both in age-related terms and final efficacy, and appears to rely on stem cell-associated pathway activation. Dissection of the molecular profiles may eventually identify targets to induce or boost regeneration in situations of (injury-related) pituitary deficiency.<\/jats:p>","DOI":"10.1210\/en.2015-1741","type":"journal-article","created":{"date-parts":[[2015,12,14]],"date-time":"2015-12-14T13:03:12Z","timestamp":1450098192000},"page":"705-721","source":"Crossref","is-referenced-by-count":50,"title":["Regeneration in the Pituitary After Cell-Ablation Injury: Time-Related Aspects and Molecular Analysis"],"prefix":"10.1210","volume":"157","author":[{"given":"Christophe","family":"Willems","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration (C.W., Q.F., H.R., F.M., B.C., J.C., H.V.), Cluster Stem Cell Biology and Embryology, Unit of Stem Cell Research, University of Leuven (KU Leuven), Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuli","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration (C.W., Q.F., H.R., F.M., B.C., J.C., H.V.), Cluster Stem Cell Biology and Embryology, Unit of Stem Cell Research, University of Leuven (KU Leuven), Leuven 3000, Belgium"},{"name":"Eye Center (Q.F.), Second Affiliated Hospital, School of Medicine, Zhejiang University, and Key Laboratory of Ophthalmology of Zhejiang Province, Hangzhou 310009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heleen","family":"Roose","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration (C.W., Q.F., H.R., F.M., B.C., J.C., H.V.), Cluster Stem Cell Biology and Embryology, Unit of Stem Cell Research, University of Leuven (KU Leuven), Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Freya","family":"Mertens","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration (C.W., Q.F., H.R., F.M., B.C., J.C., H.V.), Cluster Stem Cell Biology and Embryology, Unit of Stem Cell Research, University of Leuven (KU Leuven), Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Cox","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration (C.W., Q.F., H.R., F.M., B.C., J.C., H.V.), Cluster Stem Cell Biology and Embryology, Unit of Stem Cell Research, University of Leuven (KU Leuven), Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianghai","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration (C.W., Q.F., H.R., F.M., B.C., J.C., H.V.), Cluster Stem Cell Biology and Embryology, Unit of Stem Cell Research, University of Leuven (KU Leuven), Leuven 3000, Belgium"},{"name":"Department of Hand Surgery (J.C.), Tongji Medical College, Union Hospital, Huazhong University of Science and Technology (HUST), Wuhan, Hubei 430022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Vankelecom","sequence":"first","affiliation":[{"name":"Department of Development and Regeneration (C.W., Q.F., H.R., F.M., B.C., J.C., H.V.), Cluster Stem Cell Biology and Embryology, Unit of Stem Cell Research, University of Leuven (KU Leuven), Leuven 3000, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2015,12,14]]},"reference":[{"key":"2020071612193165300_B1","first-page":"731","volume-title":"The Pituitary","author":"Melmed","year":"2010","edition":"3rd ed"},{"key":"2020071612193165300_B2","doi-asserted-by":"crossref","first-page":"36","DOI":"10.2174\/157488812798483467","article-title":"Pituitary stem cells drop their mask","volume":"7","author":"Vankelecom","year":"2012","journal-title":"Curr Stem Cell Res 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