{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:56:05Z","timestamp":1790402165333,"version":"4.1.0"},"reference-count":48,"publisher":"The Endocrine Society","issue":"9","funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["1ZIAAG000291-10"],"award-info":[{"award-number":["1ZIAAG000291-10"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We and others have reported that taste cells in taste buds express many peptides in common with cells in the gut and islets of Langerhans in the pancreas. Islets and taste bud cells express the hormones glucagon and ghrelin, the same ATP-sensitive potassium channel responsible for depolarizing the insulin-secreting \u03b2 cell during glucose-induced insulin secretion, as well as the propeptide-processing enzymes PC1\/3 and PC2. Given the common expression of functionally specific proteins in taste buds and islets, it is surprising that no one has investigated whether insulin is synthesized in taste bud cells. Using immunofluorescence, we demonstrated the presence of insulin in mouse, rat, and human taste bud cells. By detecting the postprocessing insulin molecule C-peptide and green fluorescence protein (GFP) in taste cells of both insulin 1-GFP and insulin 2-GFP mice and the presence of the mouse insulin transcript by in situ hybridization, we further proved that insulin is synthesized in individual taste buds and not taken up from the parenchyma. In addition to our cytology data, we measured the level of insulin transcript by quantitative RT-PCR in the anterior and posterior lingual epithelia. These analyses showed that insulin is translated in the circumvallate and foliate papillae in the posterior, but only insulin transcript was detected in the anterior fungiform papillae of the rodent tongue. Thus, some taste cells are insulin-synthesizing cells generated from a continually replenished source of precursor cells in the adult mammalian lingual epithelium.<\/jats:p>","DOI":"10.1210\/en.2018-00534","type":"journal-article","created":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T18:10:44Z","timestamp":1532715044000},"page":"3331-3339","source":"Crossref","is-referenced-by-count":21,"title":["Insulin Is Transcribed and Translated in Mammalian Taste Bud Cells"],"prefix":"10.1210","volume":"159","author":[{"given":"M\u00e1ire E","family":"Doyle","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Investigation\/Diabetes Section, National Institute on Aging, National Institutes of Health, Baltimore, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L","family":"Fiori","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Investigation\/Diabetes Section, National Institute on Aging, National Institutes of Health, 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