{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:11:23Z","timestamp":1791216683788,"version":"4.1.0"},"reference-count":63,"publisher":"The Endocrine Society","issue":"12","funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01HD34283"],"award-info":[{"award-number":["R01HD34283"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01HD30428"],"award-info":[{"award-number":["R01HD30428"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R15HD078885"],"award-info":[{"award-number":["R15HD078885"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000051","name":"National Human Genome Research Institute","doi-asserted-by":"publisher","award":["T32HG000040"],"award-info":[{"award-number":["T32HG000040"]}],"id":[{"id":"10.13039\/100000051","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["T32GM007544"],"award-info":[{"award-number":["T32GM007544"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Michigan Reproductive Sciences Program","award":["U060475"],"award-info":[{"award-number":["U060475"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Transcription factors and signaling pathways that regulate stem cells and specialized hormone-producing cells in the pituitary gland have been the subject of intense study and have yielded a mechanistic understanding of pituitary organogenesis and disease. However, the regulation of stem cell proliferation and differentiation, the heterogeneity among specialized hormone-producing cells, and the role of nonendocrine cells in the gland remain important, unanswered questions. Recent advances in single-cell RNA sequencing (scRNAseq) technologies provide new avenues to address these questions. We performed scRNAseq on \u223c13,663 cells pooled from six whole pituitary glands of 7-week-old C57BL\/6 male mice. We identified pituitary endocrine and stem cells in silico, as well as other support cell types such as endothelia, connective tissue, and red and white blood cells. Differential gene expression analyses identify known and novel markers of pituitary endocrine and stem cell populations. We demonstrate the value of scRNAseq by in vivo validation of a novel gonadotrope-enriched marker, Foxp2. We present novel scRNAseq data of in vivo pituitary tissue, including data from agnostic clustering algorithms that suggest the presence of a somatotrope subpopulation enriched in sterol\/cholesterol synthesis genes. Additionally, we show that incomplete transcriptome annotation can cause false negatives on some scRNAseq platforms that only generate 3\u2032 transcript end sequences, and we use in vivo data to recover reads of the pituitary transcription factor Prop1. Ultimately, scRNAseq technologies represent a significant opportunity to address long-standing questions regarding the development and function of the different populations of the pituitary gland throughout life.<\/jats:p>","DOI":"10.1210\/en.2018-00750","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T19:42:15Z","timestamp":1539805335000},"page":"3910-3924","source":"Crossref","is-referenced-by-count":167,"title":["Single-Cell RNA Sequencing Reveals Novel Markers of Male Pituitary Stem Cells and Hormone-Producing Cell Types"],"prefix":"10.1210","volume":"159","author":[{"given":"Leonard Y M","family":"Cheung","sequence":"first","affiliation":[{"name":"Department of Human Genetics, University of Michigan, Ann Arbor, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akima S","family":"George","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Michigan, Ann Arbor, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey R","family":"McGee","sequence":"additional","affiliation":[{"name":"Department of Physiology, Southern Illinois University, Carbondale, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre Z","family":"Daly","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Michigan, Ann Arbor, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle L","family":"Brinkmeier","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Michigan, Ann Arbor, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Buffy S","family":"Ellsworth","sequence":"additional","affiliation":[{"name":"Department of Physiology, Southern Illinois University, Carbondale, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3480-896X","authenticated-orcid":false,"given":"Sally A","family":"Camper","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Michigan, Ann Arbor, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2018,10,17]]},"reference":[{"issue":"4","key":"2020071622283796900_B1","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1210\/en.2015-1862","article-title":"All hormone-producing cell types of the pituitary intermediate and anterior lobes derive from Prop1-expressing progenitors","volume":"157","author":"Davis","year":"2016","journal-title":"Endocrinology"},{"issue":"3","key":"2020071622283796900_B2","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1210\/en.2015-1836","article-title":"Molecular plasticity of male and female murine gonadotropes revealed by mRNA sequencing","volume":"157","author":"Qiao","year":"2016","journal-title":"Endocrinology"},{"issue":"30","key":"2020071622283796900_B3","doi-asserted-by":"crossref","first-page":"12515","DOI":"10.1073\/pnas.1105929108","article-title":"Related pituitary cell lineages develop into interdigitated 3D cell networks","volume":"108","author":"Budry","year":"2011","journal-title":"Proc Natl Acad Sci USA"},{"issue":"4","key":"2020071622283796900_B4","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1210\/endo.141.4.7429","article-title":"Differential expression of growth hormone messenger ribonucleic acid by somatotropes and gonadotropes in male and cycling female rats","volume":"141","author":"Childs","year":"2000","journal-title":"Endocrinology"},{"issue":"2","key":"2020071622283796900_B5","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1210\/endo-116-2-734","article-title":"Identification by plaque assays of a pituitary cell type that secretes both growth hormone and prolactin","volume":"116","author":"Frawley","year":"1985","journal-title":"Endocrinology"},{"issue":"1","key":"2020071622283796900_B6","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1038\/nri.2017.76","article-title":"Single-cell RNA sequencing to explore immune cell heterogeneity","volume":"18","author":"Papalexi","year":"2018","journal-title":"Nat Rev Immunol"},{"key":"2020071622283796900_B7","doi-asserted-by":"crossref","unstructured":"Ziegenhain\n              C\n            , ViethB, ParekhS, Reinius B, Guillaumet-Adkins A, Smets M, Leonhardt H, Heyn H, Hellmann I, Enard W. 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