{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:08:51Z","timestamp":1790050131350,"version":"4.0.1"},"reference-count":30,"publisher":"The Endocrine Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Abnormalities in lipoprotein metabolism are common in diabetes. It is unknown whether variations in form or concentration of lipoproteins influence the function of pancreatic \u03b2 cells. This study investigates whether low density lipoproteins (LDL) exhibit specific interactions with islet \u03b2 cells. Radioactively labeled LDL (125I-LDL) and fluorescently labeled LDL (DiI-LDL) were used as tracers. Rat islet cells express high affinity LDL binding sites (Kd = 9 nm), which are also recognized by very low density lipoproteins and which are down-regulated by LDL. Binding of LDL appears restricted to the \u03b2 cells, as it was not detected on islet endocrine non-\u03b2 cells. At 37 C, LDL is taken up and lysosomally degraded by islet \u03b2 cells but not by islet non-\u03b2 cells. Human islet cells were also found to present LDL binding, uptake, and degradation. Compared with rat islet cells, human islet cells exhibit 10-fold less binding sites (2.107vs. 2.108 per 103 cells) with a 2-fold lower Kd value (5 nm) and an equal sensitivity to LDL-induced down-regulation. In conclusion, human and rat islet \u03b2 cells express LDL receptors that can internalize the lipoprotein. This pathway should be examined for its potential role in (dys)regulating pancreatic \u03b2 cell functions.<\/jats:p>","DOI":"10.1210\/endo.138.10.5420","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T16:08:39Z","timestamp":1389197319000},"page":"4064-4068","source":"Crossref","is-referenced-by-count":50,"title":["Low Density Lipoprotein Binding and Uptake by Human and Rat Islet\u03b2 Cells*"],"prefix":"10.1210","volume":"138","author":[{"given":"A. Y.","family":"Grupping","sequence":"first","affiliation":[{"name":"Department of Metabolism and Endocrinology (A.Y.G., M.C., C.F.H.V.S., J.C.H., D.G.P.), Vrije Universiteit Brussel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Cnop","sequence":"additional","affiliation":[{"name":"Department of Metabolism and Endocrinology (A.Y.G., M.C., C.F.H.V.S., J.C.H., D.G.P.), Vrije Universiteit Brussel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. F. H.","family":"Van Schravendijk","sequence":"additional","affiliation":[{"name":"Department of Metabolism and Endocrinology (A.Y.G., M.C., C.F.H.V.S., J.C.H., D.G.P.), Vrije Universiteit Brussel, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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