{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:00:44Z","timestamp":1792652444333,"version":"4.5.0"},"reference-count":27,"publisher":"The Endocrine Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have investigated the effects of different growth factors on the proliferation and osteogenic potential of primary cultures of human bone marrow stromal cells (BMSC). Fibroblast growth factor (FGF)-2 was the most effective in promoting growth of these cells in vitro. The size of colonies formed in clonal conditions was approximately 2.5 times larger in presence of FGF-2. Also the morphology of BMSC was affected: cells cultured in 10% FCS alone became flattened, whereas FGF-2 expanded cells maintained a fibroblast-like elongated phenotype. Levels of alkaline phosphatase activity in BMSC expanded with FGF-2 were significantly lower (56%) than control and, after stimulation with ascorbic acid,\u03b2 Glycerophosphate and dexamethasone, FGF-2 expanded BMSC deposited approximately 3-fold more mineralized matrix than control cells. We have assessed osteogenicity of BMSC on hydroxyapatite porous scaffolds (bioceramics) by an ectopic bone formation assay. FGF-2 expanded BMSC yielded a higher bone formation (&amp;gt;20-fold) than control cells. We conclude that FGF-2, promoting BMSC proliferation, maintains cells in a more immature state allowing in vitro expansion of human osteo-progenitors which, associated with bioceramics, can differentiate in vivo and form bone tissue.<\/jats:p>","DOI":"10.1210\/endo.138.10.5425","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T16:08:39Z","timestamp":1389197319000},"page":"4456-4462","source":"Crossref","is-referenced-by-count":384,"title":["Fibroblast Growth Factor-2 Supports ex Vivo Expansion and Maintenance of Osteogenic Precursors from Human Bone Marrow*"],"prefix":"10.1210","volume":"138","author":[{"given":"Ivan","family":"Martin","sequence":"first","affiliation":[{"name":"Dipartimento di Informatica (I.M.), Sistemistica e Telematica (DIST), Universita\u0300 di Genova, 16145 Genova, Italy"},{"name":"Laboratorio di Differenziamento Cellulare (I.M., A.M., G.C., R.C., R.Q.), Istituto Nazionale per la Ricerca sul Cancro\/Centro di Biotecnologie 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