{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:13:27Z","timestamp":1792653207737,"version":"4.5.0"},"reference-count":0,"publisher":"The Endocrine Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Both human and murine osteoclasts can be derived in vitro from hematopoietic cells or monocytes that are cocultured with osteoblasts or marrow-derived stromal cells. The osteoclastogenic stimulus provided by murine osteoblasts and marrow-derived stromal cells is now known to be mediated by osteoclast differentiation factor (ODF), a membrane-bound tumor necrosis factor-related ligand. This study demonstrates that mouse spleen cells and monocytes form osteoclasts when cultured in the presence of macrophage-colony stimulating factor (M-CSF) and a soluble form of murine ODF (sODF). Numerous multinucleated osteoclasts expressing tartrate resistant acid phosphatase (TRAP) and calcitonin receptor (CTR) formed within 7 days of culture and engaged in extensive lacunar bone resorption. Osteoclast number and bone resorption area was dependent on sODF concentration. Long-term cultured human monocytes also formed bone resorbing osteoclasts in response to co-stimulation by sODF and M-CSF, although this required more than 11 days in culture. This human osteoclast differentiation was strongly inhibited by granulocyte-macrophage colony stimulating factor. This study further characterises murine osteoclast differentiation caused by sODF and M-CSF co-stimulation in vitro, and shows that the same co-stimulation causes human osteoclast differentiation to occur. We propose that this methodology can be employed to investigate the direct effects of cytokines and other factors on human osteoclast differentiation.<\/jats:p>","DOI":"10.1210\/endo.139.10.6331","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T16:08:52Z","timestamp":1389197332000},"page":"4424-4427","source":"Crossref","is-referenced-by-count":292,"title":["A Combination of Osteoclast Differentiation Factor and Macrophage-Colony Stimulating Factor Is Sufficient for both Human and Mouse Osteoclast Formation in Vitro"],"prefix":"10.1210","volume":"139","author":[{"given":"Julian M. W.","family":"Quinn","sequence":"first","affiliation":[{"name":"The University of Melbourne, Department of Medicine and St. Vincent\u2019s Institute of Medical Research, Fitzroy, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Elliott","sequence":"additional","affiliation":[{"name":"The University of Melbourne, Department of Medicine and St. Vincent\u2019s Institute of Medical Research, Fitzroy, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew T.","family":"Gillespie","sequence":"additional","affiliation":[{"name":"The University of Melbourne, Department of Medicine and St. Vincent\u2019s Institute of Medical Research, Fitzroy, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. John","family":"Martin","sequence":"additional","affiliation":[{"name":"The University of Melbourne, Department of Medicine and St. Vincent\u2019s Institute of Medical Research, Fitzroy, Australia."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","container-title":["Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/endo\/article-pdf\/139\/10\/4424\/10352619\/endo4424.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/endo\/article-pdf\/139\/10\/4424\/10352619\/endo4424.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T04:12:35Z","timestamp":1594959155000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/endo\/article\/139\/10\/4424\/2987437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,10,1]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1998,10,1]]}},"URL":"https:\/\/doi.org\/10.1210\/endo.139.10.6331","relation":{},"ISSN":["0013-7227","1945-7170"],"issn-type":[{"value":"0013-7227","type":"print"},{"value":"1945-7170","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,10,1]]}}}