{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T03:17:35Z","timestamp":1767842255694,"version":"3.49.0"},"reference-count":0,"publisher":"The Endocrine Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1999,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Although many environmental contaminants disrupt endocrine function by binding to nuclear steroid receptors, it is not known whether they are capable of binding to steroid membrane receptors and interfering with nongenomic actions of steroids. The binding of several organochlorine pesticides to the plasma membrane receptor for the maturation-inducing steroid, 17,20\u03b2,21-trihydroxy-4-pregnen-3-one (20\u03b2-S), in the ovaries of spotted seatrout (Cynoscion nebulosus) was investigated in in vitro competition assays. Kepone and o,p\u2032-DDD were competitive inhibitors of 20\u03b2-S binding and caused concentration-dependent displacement of [3H]-20\u03b2-S from its receptor site over the range of 10\u22124 to 10\u22126 or 10\u22127 M, whereas several other pesticides had lower affinities for the receptor. Interference with the nongenomic actions of 20\u03b2-S on final meiotic maturation of spotted seatrout oocytes (final oocyte maturation, FOM) was examined in an in vitro bioassay. A concentration-dependent inhibition of FOM in response to 20\u03b2-S was observed after 5 min and 12 h exposure to the same range of Kepone and o,p\u2032-DDD concentrations (10\u22124 to 10\u22126 or 10\u22127 M). The close correspondence between competitive binding of the two pesticides to the 20\u03b2-S membrane receptor and their inhibition of 20\u03b2-S induced FOM suggests a mechanism of endocrine disruption mediated by binding to a steroid membrane receptor and antagonism of a nongenomic steroid action.<\/jats:p>","DOI":"10.1210\/endo.140.4.6781","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T16:10:00Z","timestamp":1389197400000},"page":"1953-1956","source":"Crossref","is-referenced-by-count":37,"title":["Pesticides Interfere with the Nongenomic Action of a Progestogen on Meiotic Maturation by Binding to its Plasma Membrane Receptor on Fish Oocytes"],"prefix":"10.1210","volume":"140","author":[{"given":"Shampa","family":"Das","sequence":"first","affiliation":[{"name":"Department of Internal Medicine (S.D.), Yale University, School of Medicine, VA Medical Center, West Haven, Connecticut 06516; University of Texas at Austin, Marine Science Institute, Port Aransas, Texas 78373-5015"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Thomas","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine (S.D.), Yale University, School of Medicine, VA Medical Center, West Haven, Connecticut 06516; University of Texas at Austin, Marine Science Institute, Port Aransas, Texas 78373-5015"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","container-title":["Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/endo\/article-pdf\/140\/4\/1953\/10380699\/endo1953.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/endo\/article-pdf\/140\/4\/1953\/10380699\/endo1953.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T04:42:33Z","timestamp":1594960953000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/endo\/article\/140\/4\/1953\/2990798"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1999,4,1]]}},"URL":"https:\/\/doi.org\/10.1210\/endo.140.4.6781","relation":{},"ISSN":["0013-7227","1945-7170"],"issn-type":[{"value":"0013-7227","type":"print"},{"value":"1945-7170","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,4,1]]}}}