{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T12:10:03Z","timestamp":1746101403144,"version":"3.40.4"},"reference-count":58,"publisher":"The Endocrine Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present study examines Ca2+ second messenger signaling driven by LH in isolated porcine thecal cells. To this end, we implemented semiquantitative fluorescent (fura-2) videomicroscopic imaging of single thecal cells in vitro. Stimulation of 388 cells with LH (5 \u03bcg\/ml) elicited an intracellular Ca2+ ([Ca2+]i) signal in 85\u00b1 5.3% of individual thecal cells (n = 11 experiments). Among 337 LH-responsive cells, we identified four predominant temporal modes of [Ca2+]i signaling: 1)[ Ca2+]i oscillations with periodicities of 0.5 to 4.5 min\u22121 (63 \u00b1 4.5%), 2) a[ Ca2+]i spike followed by a sustained plateau (17 \u00b1 2.6%), 3) a [Ca2+]i spike only (5.8 \u00b1 2.6%); and 4) a [Ca2+]i plateau only (3.8 \u00b1 1.5%). The prevalence, but not the amplitude or frequency, of LH-induced [Ca2+]i oscillations in thecal cells was dependent on the agonist concentration. Reduced availability of extracellular Ca2+ induced by treatment with EGTA or cobaltous chloride did not block the initiation, but reversibly abolished ongoing [Ca2+]i oscillations (72% of cells) or increased the mean[ Ca2+]i interspike periodicity from 1.09\u00b1 0.16 to 0.59 \u00b1 0.07 min\u22121 (P&amp;lt; 0.05). Putative phospholipase C inhibition with U-73122 (10\u03bc m) also abolished or frequency-damped LH-driven[ Ca2+]i oscillations in 95 \u00b1 4.7% of cells. [Ca2+]i oscillations in thecal cells were not abrogated by overnight pretreatment with pertussis toxin. We conclude that 1) thecal cells (unlike earlier findings in granulosa cells) manifest a diverse array of [Ca2+]i signaling responses to LH at the single cell level; 2) LH can dose dependently recruit an increasing number of individually[ Ca2+]i oscillating thecal cells; 3) extracellular Ca2+ is required for LH to sustain (but not initiate) frequent and high amplitude [Ca2+] oscillations in thecal cells; and 4) these signaling actions of LH are mediated via phospholipase C, but not a pertussis-toxin sensitive mechanism. Accordingly, the present data extend the apparent complexity of LH-induced [Ca2+]i second messenger signaling and identify at the single cell level LH\u2019s dose-responsive drive of[ Ca2+]i oscillations in gonadal cells.<\/jats:p>","DOI":"10.1210\/endo.141.6.7501","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T16:11:03Z","timestamp":1389197463000},"page":"2220-2228","source":"Crossref","is-referenced-by-count":8,"title":["Luteinizing Hormone (LH) Drives Diverse Intracellular Calcium Second Messenger Signals in Isolated Porcine Ovarian Thecal Cells: Preferential Recruitment of Intracellular Ca2+ Oscillatory Cells by Higher Concentrations of LH*"],"prefix":"10.1210","volume":"141","author":[{"given":"C.","family":"Aguirre","sequence":"first","affiliation":[{"name":"Division of Endocrinology, Department of Internal Medicine, National Science Foundation Center for Biological Timing, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. C. L.","family":"Jayes","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Department of Internal Medicine, National Science Foundation Center for Biological Timing, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. D.","family":"Veldhuis","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Department of Internal Medicine, National Science Foundation Center for Biological Timing, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"key":"2020071621350452800_R1","first-page":"137","article-title":"The role of cyclic AMP in gonadal function","author":"Marsh","year":"1975","journal-title":"In: Greengard P, Robison GA (eds) Advances in Cyclic Nucleotide Research. 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