{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T16:37:45Z","timestamp":1789749465752,"version":"4.0.1"},"reference-count":54,"publisher":"The Endocrine Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>PGs are regulators of a plethora of uterine functions during reproductive processes, including uterine contractility. In bovine uterus, the rate-limiting step in PG synthesis is catalyzed by the PG endoperoxide G\/H synthase (PGHS) enzymes. It has previously been established that PGHS-2 isoform expression is affected by the ruminant-specific interferon (IFN)-\u03c4 in bovine endometrial cells. Here, we show that PGHS-2 mRNA and protein levels are induced by IFN-\u03c4 in primary cell cultures from bovine myometrium. Treatment with recombinant bovine IFN-\u03c4 induces the activation of the JAK-STAT and p38 MAPK pathways in bovine myometrial cells. Inhibition of the p38 pathway by the specific inhibitor SB203580 strongly decreases PGHS-2 mRNA and protein expression without affecting the phosphorylation and DNA-binding of transcription factors STAT-1 and STAT-2. The p38 pathway regulates PGHS-2 expression at the posttranscriptional level, because the presence of SB203580 results in the destabilization of IFN-\u03c4-induced PGHS-2 mRNA. Taken together, these data demonstrate the ability of IFN-\u03c4 to induce the activation of the JAK-STAT pathway in a manner similar to other types of IFN (i.e. \u03b1, \u03b2, and \u03b3) and to regulate PGHS-2 mRNA stability through the activation of the p38 pathway. These findings provide new insights into the physiological function of IFN-\u03c4, in regard to regulation of specific genes associated with myometrial contractility.<\/jats:p>","DOI":"10.1210\/endo.142.12.8524","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T16:11:14Z","timestamp":1389197474000},"page":"5107-5115","source":"Crossref","is-referenced-by-count":12,"title":["Induction of PG G\/H Synthase-2 in Bovine Myometrial Cells by Interferon-\u03c4 Requires the Activation of the p38 MAPK Pathway"],"prefix":"10.1210","volume":"142","author":[{"given":"Florence","family":"Doualla-Bell","sequence":"first","affiliation":[{"name":"Perinatal Research and Developmental Pharmacology Unit (F.D.-B.), Lady Davis Institute for Medical Research, Sir Mortimer B. 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