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Adjudin was shown to exert its effects preferentially by perturbing the testis-specific actin-rich adherens junction (AJ) at the Sertoli\u2013spermatid interface known as apical ectoplasmic specialization (apical ES), thereby effectively inducing spermatid exfoliation. Adjudin did not perturb germ cell development nor germ cell function. Also, it had no effects on Sertoli cell\u2013cell AJ called basal ectoplasmic specialization (basal ES), which, together with tight junction constitute the blood-testis barrier (BTB), unless an acute dose of adjudin was used. Adjudin also did not perturb the population of spermatogonial stem cells nor Sertoli cells in the testis. However, the downstream signaling protein(s) utilized by adjudin to induce transient male infertility remains unexplored. Herein, using adult rats treated with adjudin and monitored changes in the phenotypes across the seminiferous epithelium between 6 and 96 h in parallel with the steady-state protein levels of an array of signaling and cytoskeletal regulatory proteins, recently shown to be involved in apical ES, basal ES and BTB function. It was shown that adjudin exerts its contraceptive effects through changes in microtubule associated proteins (MAPs) and signaling proteins mTORC1\/rpS6 and p-FAK-Y407. These findings are important to not only study adjudin-mediated male infertility but also the biology of spermatogenesis.<\/jats:p>","DOI":"10.1210\/endocr\/bqaa196","type":"journal-article","created":{"date-parts":[[2020,10,23]],"date-time":"2020-10-23T07:00:38Z","timestamp":1603436438000},"source":"Crossref","is-referenced-by-count":15,"title":["The Non-hormonal Male Contraceptive Adjudin Exerts its Effects via MAPs and Signaling Proteins mTORC1\/rpS6 and FAK-Y407"],"prefix":"10.1210","volume":"162","author":[{"given":"Lingling","family":"Wang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, Nanjing, Jiangsu, China"},{"name":"The Second Affiliated Hospital and Yuying Children\u2019s Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China"},{"name":"The Mary M. Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Drug Screening, China Pharmaceutical University, Nanjing, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huitao","family":"Li","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital and Yuying Children\u2019s Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siwen","family":"Wu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital and Yuying Children\u2019s Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renshan","family":"Ge","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital and Yuying Children\u2019s Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris K C","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Biology, Croucher Institute for Environmental Sciences, Hong Kong Baptist University, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Silvestrini","sequence":"additional","affiliation":[{"name":"S.B.M. 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Wohlford Laboratory for Male Contraceptive Research, Center for Biomedical Research, Population Council, New York, New York"},{"name":"Institute of Reproductive Medicine, Nantong University School of Medicine, Nantong, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2020,10,23]]},"reference":[{"issue":"4","key":"2021121701454973500_CIT0001","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1002\/jemt.20786","article-title":"Surfing the wave, cycle, life history, and genes\/proteins expressed by testicular germ cells. 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