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The complexity of PA diagnosis encourages the identification of novel PA biomarkers. Urinary extracellular vesicles (uEVs) are a potential source of biomarkers, considering that their cargo reflects the content of the parent cell.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to evaluate the proteome of uEVs from PA patients and identify potential biomarker candidates for PA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Second morning spot urine was collected from healthy controls (n\u2005=\u20058) and PA patients (n\u2005=\u20057). The uEVs were isolated by ultracentrifugation and characterized. Proteomic analysis on uEVs was performed using LC-MS Orbitrap.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Isolated uEVs carried extracellular vesicle markers, showed a round shape and sizes between 50 and 150 nm. The concentration of uEVs showed a direct correlation with urinary creatinine (r\u2005=\u20050.6357; P = 0.0128). The uEV size mean (167\u2005\u00b1\u20056 vs 183\u2005\u00b1\u20054nm) and mode (137\u2005\u00b1\u20057 vs 171\u2005\u00b1\u200511nm) was significantly smaller in PA patients than in control subjects, but similar in concentration. Proteomic analysis of uEVs from PA patients identified an upregulation of alpha-1-acid glycoprotein 1 (AGP1) in PA uEVs, which was confirmed using immunoblot. A receiver operating characteristic curve analysis showed an area under the curve of 0.92 (0.82 to 1; P = 0.0055).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Proteomic and further immunoblot analyses of uEVs highlights AGP1 as potential biomarker for PA.<\/jats:p><\/jats:sec>","DOI":"10.1210\/endocr\/bqab032","type":"journal-article","created":{"date-parts":[[2021,2,14]],"date-time":"2021-02-14T08:04:36Z","timestamp":1613289876000},"source":"Crossref","is-referenced-by-count":19,"title":["Proteomic Profile of Urinary Extracellular Vesicles Identifies AGP1 as a Potential Biomarker of Primary Aldosteronism"],"prefix":"10.1210","volume":"162","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6613-4181","authenticated-orcid":false,"given":"Eric R","family":"Barros","sequence":"first","affiliation":[{"name":"Department of Endocrinology, School of Medicine, Pontificia Universidad Cat\u00f3lica de Chile, Santiago, Chile"},{"name":"Department of Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, HB Nijmegen, The Netherlands"},{"name":"Center for Translational Research in Endocrinology (CETREN-UC), Pontificia Universidad Cat\u00f3lica de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4168-9396","authenticated-orcid":false,"given":"Juan Pablo","family":"Rigalli","sequence":"additional","affiliation":[{"name":"Department of Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, HB Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6081-1468","authenticated-orcid":false,"given":"Alejandra","family":"Tapia-Castillo","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, School of Medicine, Pontificia Universidad Cat\u00f3lica de Chile, Santiago, Chile"},{"name":"Center for Translational Research in Endocrinology (CETREN-UC), Pontificia Universidad Cat\u00f3lica de Chile, Santiago, Chile"},{"name":"Millennium Institute of Immunology and Immunotherapy (IMII-ICM), Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Vecchiola","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, School of Medicine, Pontificia Universidad Cat\u00f3lica de Chile, Santiago, Chile"},{"name":"Center for Translational Research in Endocrinology (CETREN-UC), Pontificia Universidad Cat\u00f3lica de Chile, Santiago, Chile"},{"name":"Millennium Institute of Immunology and Immunotherapy (IMII-ICM), Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0450-5375","authenticated-orcid":false,"given":"Morag J","family":"Young","sequence":"additional","affiliation":[{"name":"Baker Heart and Diabetes Institute, Melbourne, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost G J","family":"Hoenderop","sequence":"additional","affiliation":[{"name":"Department of Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, HB Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9 J M","family":"Bindels","sequence":"additional","affiliation":[{"name":"Department of Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, HB Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos E","family":"Fardella","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, School of Medicine, Pontificia Universidad Cat\u00f3lica de Chile, Santiago, Chile"},{"name":"Center for Translational Research in Endocrinology (CETREN-UC), Pontificia Universidad Cat\u00f3lica de Chile, Santiago, Chile"},{"name":"Millennium Institute 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