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The composition of seminal plasma contributes to paternal programming effects through modulating the female reproductive tract immune response after mating. To investigate whether paternal obesity affects seminal plasma immune-regulatory activity, C57Bl\/6 male mice were fed an obesogenic high-fat diet (HFD) or control diet (CD) for 14 weeks. Although HFD consumption caused only minor changes to parameters of sperm quality, the volume of seminal vesicle fluid secretions was increased by 65%, and the concentrations and total content of immune-regulatory TGF-\u03b2 isoforms were decreased by 75% to 80% and 43% to 55%, respectively. Mating with BALB\/c females revealed differences in the strength and properties of the postmating immune response elicited. Transcriptional analysis showed &amp;gt;300 inflammatory genes were similarly regulated in the uterine endometrium by mating independently of paternal diet, and 13 were dysregulated by HFD-fed compared with CD-fed males. Seminal vesicle fluid factors reduced in HFD-fed males, including TGF-\u03b21, IL-10, and TNF, were among the predicted upstream regulators of differentially regulated genes. Additionally, the T-cell response induced by mating with CD-fed males was blunted after mating with HFD-fed males, with 27% fewer CD4+ T cells, 26% fewer FOXP3+CD4+ regulatory T cells (Treg) cells, and 19% fewer CTLA4+ Treg cells, particularly within the NRP1+ thymic Treg cell population. These findings demonstrate that an obesogenic HFD alters the composition of seminal vesicle fluid and impairs seminal plasma capacity to elicit a favorable pro-tolerogenic immune response in females at conception.<\/jats:p>","DOI":"10.1210\/endocr\/bqab123","type":"journal-article","created":{"date-parts":[[2021,6,25]],"date-time":"2021-06-25T15:42:55Z","timestamp":1624635775000},"source":"Crossref","is-referenced-by-count":43,"title":["High-fat Diet Alters Male Seminal Plasma Composition to Impair Female Immune Adaptation for Pregnancy in Mice"],"prefix":"10.1210","volume":"162","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6293-6160","authenticated-orcid":false,"given":"John E","family":"Schjenken","sequence":"first","affiliation":[{"name":"The Robinson Research Institute, Adelaide Medical School, University of Adelaide, Adelaide, South Australia, 5005, Australia"},{"name":"Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, The University of Newcastle, University Drive, Callaghan, NSW, 2308, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3141-2521","authenticated-orcid":false,"given":"Lachlan M","family":"Moldenhauer","sequence":"additional","affiliation":[{"name":"The Robinson Research Institute, Adelaide Medical School, University of Adelaide, Adelaide, South Australia, 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4831-7950","authenticated-orcid":false,"given":"David J","family":"Sharkey","sequence":"additional","affiliation":[{"name":"The Robinson Research Institute, Adelaide Medical School, University of Adelaide, Adelaide, South Australia, 5005, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9841-2094","authenticated-orcid":false,"given":"Hon Y","family":"Chan","sequence":"additional","affiliation":[{"name":"The Robinson 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