{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:46:59Z","timestamp":1787021219684,"version":"build-2736575974"},"reference-count":88,"publisher":"The Endocrine Society","issue":"9","license":[{"start":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T00:00:00Z","timestamp":1656633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Polycystic ovarian syndrome (PCOS) is a complex health condition associated with metabolic disturbances and infertility. Recent data suggest that the prevalence of PCOS is increasing among women globally, although the etiology of these trends is undefined. Consequently, preclinical models that better reflect the biology of PCOS are urgently needed to facilitate research that can lead to the discovery of prevention strategies or improved management. The existing animal models have several limitations as they do not reflect all the PCOS features metabolically and\/or phenotypically. Therefore, there is no clear consensus on the use of appropriate animal model and selection of the most appropriate PCOS-inducing agent. To that end, we have established a Swiss albino mouse model of PCOS based on 3 weeks of daily treatment with letrozole (50 \u03bcg\/day; intraperitoneal) and dehydroepiandrosterone (DHEA, 6 mg\/100 g body weight; subcutaneous) in 5-week-old female mice fed on normal or high-fat diet (HFD). Mice were regularly assessed for body weight, blood glucose, and estrous cycle. Three weeks after drug administration, mice were sacrificed and assessed for blood-based metabolic parameters as well as ovarian function. Our results indicate that DHEA combined with HFD produces changes mimicking those of clinical PCOS, including elevated serum testosterone and luteinizing hormone, dyslipidemia, poor ovarian microenvironment, and development of multiple ovarian cysts, recapitulating cardinal features of PCOS. In comparison, normal diet and\/or letrozole produced fewer features of PCOS. The data from the experimental models presented here can improve our understanding of PCOS, a growing concern in women\u2019s health.<\/jats:p>","DOI":"10.1210\/endocr\/bqac097","type":"journal-article","created":{"date-parts":[[2022,7,1]],"date-time":"2022-07-01T11:47:22Z","timestamp":1656676042000},"source":"Crossref","is-referenced-by-count":31,"title":["Distinctions in PCOS Induced by Letrozole Vs Dehydroepiandrosterone With High-fat Diet in Mouse Model"],"prefix":"10.1210","volume":"163","author":[{"given":"Pooja Suresh","family":"Poojary","sequence":"first","affiliation":[{"name":"Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal-576104, Karnataka , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guruprasad","family":"Nayak","sequence":"additional","affiliation":[{"name":"Division of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal-576104, Karnataka , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gangotri","family":"Panchanan","sequence":"additional","affiliation":[{"name":"Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal-576104, Karnataka , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arpitha","family":"Rao","sequence":"additional","affiliation":[{"name":"Division of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal-576104, Karnataka , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjna Das","family":"Kundapur","sequence":"additional","affiliation":[{"name":"Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal-576104, Karnataka , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sneha Guruprasad","family":"Kalthur","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal-576104, Karnataka , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0642-1928","authenticated-orcid":false,"given":"Srinivas","family":"Mutalik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education , Manipal-576104, Karnataka , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish Kumar","family":"Adiga","sequence":"additional","affiliation":[{"name":"Division of Clinical Embryology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal-576104, Karnataka , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology and Department of Laboratory Medicine and Pathology, Mayo Clinic , Rochester, MN 55902 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Bakkum-Gamez","sequence":"additional","affiliation":[{"name":"Division of Laboratory Genetics and Genomics, Department of Laboratory Medicine and Pathology, Mayo Clinic , Rochester, MN 55902 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice Y","family":"Chang","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Oncology Surgery, Department of Obstetrics and Gynecology, Mayo Clinic , Rochester, MN 55902 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"DeStephano","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Diabetes, Metabolism, Nutrition, Department of Internal Medicine, Mayo Clinic , Rochester, MN 55902 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Sherman","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences, Mayo Clinic , Jacksonville, FL 32224 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8825-2178","authenticated-orcid":false,"given":"Nagarajan","family":"Kannan","sequence":"additional","affiliation":[{"name":"Division of Experimental Pathology and Laboratory Medicine, Department of Laboratory Medicine and Pathology, Mayo Clinic , Rochester, MN 55902 , USA"},{"name":"Mayo Clinic Cancer Center, Mayo Clinic , Rochester, MN 55902 , USA"},{"name":"Center for Regenerative Medicine, Mayo Clinic , Rochester, MN 55902 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4554-2917","authenticated-orcid":false,"given":"Guruprasad","family":"Kalthur","sequence":"additional","affiliation":[{"name":"Division of Reproductive Biology, Department of Reproductive Science, Kasturba Medical College, Manipal, Manipal Academy of Higher Education , Manipal-576104, Karnataka , India"},{"name":"Division of Experimental Pathology and Laboratory Medicine, Department of Laboratory Medicine and Pathology, Mayo Clinic , Rochester, MN 55902 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2022,7,1]]},"reference":[{"issue":"5","key":"2022072509193886500_CIT0001","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1038\/nrendo.2018.24","article-title":"Polycystic ovary syndrome: definition, aetiology, diagnosis and treatment","volume":"14","author":"Escobar-Morreale","year":"2018","journal-title":"Nat Rev 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