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To measure changes of the cytosolic ATP\/ADP ratio, single alpha-cells and beta-cells from NMRI mice were adenovirally transduced with the fluorescent indicator PercevalHR. The cytosolic Ca2+ concentration ([Ca2+]i) was measured by use of Fura2 and the mitochondrial membrane potential by use of TMRE. Perifused islets were used to measure the secretion of glucagon and insulin. At 5 mM glucose, the PercevalHR ratio in beta-cells was significantly lower than in alpha-cells. Lowering glucose to 1 mM decreased the ratio to 69% within 10 minutes in beta-cells, but only to 94% in alpha-cells. In this situation, 30 mM glucose, 10 mM alpha-ketoisocaproic acid, and 10 mM glutamine plus 10 mM BCH (a nonmetabolizable leucine analogue) markedly increased the PercevalHR ratio in beta-cells. In alpha-cells, only glucose was slightly effective. However, none of the nutrients increased the mitochondrial membrane potential in alpha-cells, whereas all did so in beta-cells. The kinetics of the PercevalHR increase were reflected by the kinetics of [Ca2+]i. increase in the beta-cells and insulin secretion. Glucagon secretion was markedly increased by washing out the nutrients with 1 mM glucose, but not by reducing glucose from 5 mM to 1 mM. This pattern was still recognizable when the insulin secretion was strongly inhibited by clonidine. It is concluded that mitochondrial energy metabolism is a signal generator in pancreatic beta-cells, but not in alpha-cells.<\/jats:p>","DOI":"10.1210\/endocr\/bqac125","type":"journal-article","created":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T22:19:09Z","timestamp":1659737949000},"source":"Crossref","is-referenced-by-count":8,"title":["Metabolic Regulation of Hormone Secretion in Beta-Cells and Alpha-Cells of Female Mice: Fundamental Differences"],"prefix":"10.1210","volume":"163","author":[{"given":"Dennis","family":"Br\u00fcning","sequence":"first","affiliation":[{"name":"Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universit\u00e4t Braunschweig , D 38106 Braunschweig , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mai","family":"Morsi","sequence":"additional","affiliation":[{"name":"Institute of Pharmacology, Toxicology and Clinical Pharmacy, Technische Universit\u00e4t Braunschweig , D 38106 Braunschweig , 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