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It secretes hormones in response to hypothalamic input, end organ feedback, and diurnal cues. The mechanisms by which pituitary stem cells are recruited to proliferate, maintain quiescence, or differentiate into specific cell types, especially thyrotropes, are not well understood. We used single-cell RNA sequencing in juvenile P7 mouse pituitary cells to identify novel factors in pituitary cell populations, with a focus on thyrotropes and rare subtypes. We first observed cells coexpressing markers of both thyrotropes and gonadotropes, such as Pou1f1 and Nr5a1. This was validated in vivo by both immunohistochemistry and lineage tracing of thyrotropes derived from Nr5a1-Cre; mTmG mice and demonstrates that Nr5a1-progenitors give rise to a proportion of thyrotropes during development. Our data set also identifies novel factors expressed in pars distalis and pars tuberalis thyrotropes, including the Shox2b isoform in all thyrotropes and Sox14 specifically in Pou1f1-negative pars tuberalis thyrotropes. We have therefore used single-cell transcriptomics to determine a novel developmental trajectory for thyrotropes and potential novel regulators of thyrotrope populations.<\/jats:p>","DOI":"10.1210\/endocr\/bqad076","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T06:49:06Z","timestamp":1684133346000},"source":"Crossref","is-referenced-by-count":16,"title":["Novel Candidate Regulators and Developmental Trajectory of Pituitary Thyrotropes"],"prefix":"10.1210","volume":"164","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0912-9594","authenticated-orcid":false,"given":"Leonard Y M","family":"Cheung","sequence":"first","affiliation":[{"name":"Department of Human Genetics, University of Michigan , Ann Arbor, MI 48109 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Menage","sequence":"additional","affiliation":[{"name":"School of Neuroscience, Institute of Psychiatry, Psychology and Neuroscience, King's College London , London SE5 8AF , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Rizzoti","sequence":"additional","affiliation":[{"name":"Laboratory of Stem Cell Biology and Developmental Genetics, The Francis Crick Institute , London NW1 1AT , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Hamilton","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Calgary , Calgary AB T2N 1N4 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Typhanie","family":"Dumontet","sequence":"additional","affiliation":[{"name":"Training Program in Organogenesis, Center for Cell Plasticity and Organ Design, University of Michigan , Ann Arbor, MI 48109 , USA"},{"name":"Department of Internal Medicine, Division of Metabolism, Endocrinology, and Diabetes, University of Michigan , Ann Arbor, MI 48109 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaitlin","family":"Basham","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Metabolism, Endocrinology, and Diabetes, University of Michigan , Ann Arbor, MI 48109 , USA"},{"name":"Current affiliation: Huntsman Cancer Institute, University of Utah , Salt Lake City, UT 84112 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre Z","family":"Daly","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Michigan , Ann Arbor, MI 48109 , USA"},{"name":"Current affiliation is Vanguard , Valley Forge, PA 19482 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle L","family":"Brinkmeier","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Michigan , Ann Arbor, MI 48109 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bailey E","family":"Masser","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University of Michigan , Ann Arbor, MI 48109 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Treier","sequence":"additional","affiliation":[{"name":"Max Delbr\u03cbck Center for Molecular Medicine (MDC) , 13092 Berlin , Germany"},{"name":"Charit\u00e9-Universit\u00e4tsmedizin Berlin , 10117 Berlin , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Cobb","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Calgary , Calgary AB T2N 1N4 , Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessio","family":"Delogu","sequence":"additional","affiliation":[{"name":"School of Neuroscience, Institute of Psychiatry, Psychology and Neuroscience, King's College London , London SE5 8AF , 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