{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:06:01Z","timestamp":1792811161723,"version":"4.5.0"},"reference-count":45,"publisher":"The Endocrine Society","issue":"1","license":[{"start":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T00:00:00Z","timestamp":1699228800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01HD097087"],"award-info":[{"award-number":["R01HD097087"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R01HD097087-02S1"],"award-info":[{"award-number":["R01HD097087-02S1"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["F31HD107807"],"award-info":[{"award-number":["F31HD107807"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R35HL145242"],"award-info":[{"award-number":["R35HL145242"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Department of Defense TTDA","award":["W81XWH2010603"],"award-info":[{"award-number":["W81XWH2010603"]}]},{"name":"Department of Defense TTDA","award":["W81XWH2210281"],"award-info":[{"award-number":["W81XWH2210281"]}]},{"name":"NIH-funded WSU Maximizing Access to Research Careers"},{"name":"Barry Goldwater"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Ciliary action performs a critical role in the oviduct (Fallopian tube) during pregnancy establishment through sperm and egg transport. The disruption of normal ciliary function in the oviduct affects oocyte pick-up and is a contributing factor to female infertility. Estrogen is an important regulator of ciliary action in the oviduct and promotes ciliogenesis in several species. Global loss of estrogen receptor \u03b1 (ESR1) leads to infertility. We have previously shown that ESR1 in the oviductal epithelial cell layer is required for female fertility. Here, we assessed the role of estrogen on transcriptional regulation of ciliated epithelial cells of the oviduct using single-cell RNA-sequencing analysis. We observed minor variations in ciliated cell genes in the proximal region (isthmus and uterotubal junction) of the oviduct. However, 17\u03b2-estradiol treatment had little impact on the gene expression profile of ciliated epithelial cells. We also conditionally ablated Esr1 from ciliated epithelial cells of the oviduct (called ciliated Esr1d\/d mice). Our studies showed that ciliated Esr1d\/d females had fertility rates comparable to control females, did not display any disruptions in preimplantation embryo development or embryo transport to the uterus, and had comparable cilia formation to control females. However, we observed some incomplete deletion of Esr1 in the ciliated epithelial cells, especially in the ampulla region. Nevertheless, our data suggest that ESR1 expression in ciliated cells of the oviduct is dispensable for ciliogenesis and nonessential for female fertility in mice.<\/jats:p>","DOI":"10.1210\/endocr\/bqad163","type":"journal-article","created":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T12:10:14Z","timestamp":1699531814000},"source":"Crossref","is-referenced-by-count":14,"title":["Classical Estrogen Signaling in Ciliated Epithelial Cells of the Oviduct Is Nonessential for Fertility in Female Mice"],"prefix":"10.1210","volume":"165","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4979-9979","authenticated-orcid":false,"given":"Emily A","family":"McGlade","sequence":"first","affiliation":[{"name":"Obstetrics, Gynecology and Women's Health, School of Medicine, University of Missouri , Columbia, MO 65211 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kalli K","family":"Stephens","sequence":"additional","affiliation":[{"name":"Obstetrics, Gynecology and Women's Health, School of Medicine, University of Missouri , Columbia, MO 65211 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8439-0389","authenticated-orcid":false,"given":"Sarayut","family":"Winuthayanon","sequence":"additional","affiliation":[{"name":"Division of Animal Sciences, University of Missouri , Columbia, MO 65211 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0664-9746","authenticated-orcid":false,"given":"Prashanth","family":"Anamthathmakula","sequence":"additional","affiliation":[{"name":"School of Medicine, University of Missouri-Kansas City , Kansas City, MO 64108 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8750-3716","authenticated-orcid":false,"given":"Michael J","family":"Holtzman","sequence":"additional","affiliation":[{"name":"Pulmonary and Critical Care Medicine, Department of Medicine, Washington University School of Medicine , St.Louis, MO 63110 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5196-8471","authenticated-orcid":false,"given":"Wipawee","family":"Winuthayanon","sequence":"additional","affiliation":[{"name":"Obstetrics, Gynecology and Women's Health, School of Medicine, University of Missouri , Columbia, MO 65211 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2023,11,6]]},"reference":[{"issue":"19","key":"2023122315532913600_bqad163-B1","doi-asserted-by":"crossref","first-page":"R973","DOI":"10.1016\/j.cub.2014.08.047","article-title":"Multiciliated cells","volume":"24","author":"Brooks","year":"2014","journal-title":"Curr Biol"},{"issue":"12","key":"2023122315532913600_bqad163-B2","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1002\/mrd.23650","article-title":"Structures and functions of cilia during vertebrate embryo development","volume":"89","author":"Amack","year":"2022","journal-title":"Mol Reprod Dev"},{"issue":"4","key":"2023122315532913600_bqad163-B3","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1093\/humupd\/dml012","article-title":"The reproductive significance of human Fallopian tube cilia","volume":"12","author":"Lyons","year":"2006","journal-title":"Hum Reprod Update"},{"issue":"6","key":"2023122315532913600_bqad163-B4","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1111\/j.1479-828X.2006.00653.x","article-title":"Risk factors for ectopic pregnancy: a case-control study","volume":"46","author":"Karaer","year":"2006","journal-title":"Aust N Z J Obstet Gynaecol"},{"issue":"1","key":"2023122315532913600_bqad163-B5","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1186\/s12884-015-0613-1","article-title":"Risk factors for ectopic pregnancy: a multi-center case-control study","volume":"15","author":"Li","year":"2015","journal-title":"BMC Pregnancy Childbirth"},{"issue":"1","key":"2023122315532913600_bqad163-B6","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1093\/humrep\/12.1.55","article-title":"Function and structure of cilia in the fallopian tube of an infertile woman with Kartagener's Syndrome","volume":"12","author":"Halbert","year":"1997","journal-title":"Hum Reprod"},{"issue":"3","key":"2023122315532913600_bqad163-B7","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/S0015-0282(16)49578-6","article-title":"The oviductal cilia and Kartagener's Syndrome","volume":"46","author":"McComb","year":"1986","journal-title":"Fertil Steril"},{"issue":"3","key":"2023122315532913600_bqad163-B8","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1378\/chest.95.3.578","article-title":"Ciliary ultrastructure of respiratory and fallopian tube epithelium in a sterile woman with Kartagener's Syndrome. 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