{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T10:11:14Z","timestamp":1784628674877,"version":"3.55.0"},"reference-count":57,"publisher":"The Endocrine Society","issue":"4","license":[{"start":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T00:00:00Z","timestamp":1706659200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["267610"],"award-info":[{"award-number":["267610"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["245979"],"award-info":[{"award-number":["245979"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["254870"],"award-info":[{"award-number":["254870"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005416","name":"Research Council of Norway","doi-asserted-by":"publisher","award":["336213"],"award-info":[{"award-number":["336213"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Early puberty poses a significant challenge for male Atlantic salmon in aquaculture due to its negative impact on growth and welfare. The regulation of puberty in vertebrates involves 2 key reproductive hormones: follicle-stimulating hormone (FSH) and luteinizing hormone (LH) and their gonadal receptors. In male mice lacking FSH receptor, testes size is reduced, but fertility is maintained, while medaka and zebrafish with a disrupted fshr gene exhibit near normal testis size and fertility. In these fishes both Fsh and Lh are present during puberty and Lh may rescue fertility, while in salmonid fish only Fsh is present in the circulation during puberty. Using CRISPR-Cas9, we produced crispants with a high prevalence of fshr mutations at the target site, which remained fertile, although more than half showed a testis development deviating from wild-type (wt) males. Crossing out these F0 crispants to each other produced a viable F1 generation showing frameshift (fshr\u2212\/\u2212) or in-frame mutations (fshrif\/if). Nearly all wt males matured while all fshr\u2212\/\u2212 males remained immature with small testes containing A spermatogonia as the furthest developed germ cell type and prepubertal plasma androgen levels. Also, the pituitary transcript levels of gnrhr2bba and lhb, but not for fshb, were reduced in the fshr\u2212\/\u2212 males compared with maturing males. More than half of the fshrif\/if mutant males showed no or a delayed maturation. In conclusion, Atlantic salmon show the unique characteristic that loss of Fshr function alone results in male infertility, offering new opportunities to control precocious puberty or fertility in salmon.<\/jats:p>","DOI":"10.1210\/endocr\/bqae013","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T08:43:43Z","timestamp":1706777023000},"source":"Crossref","is-referenced-by-count":22,"title":["Loss of Fshr Prevents Testicular Maturation in Atlantic Salmon (<i>Salmo salar<\/i> L.)"],"prefix":"10.1210","volume":"165","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7864-1014","authenticated-orcid":false,"given":"Eva","family":"Andersson","sequence":"first","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9115-669X","authenticated-orcid":false,"given":"R\u00fcdiger W","family":"Schulz","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"},{"name":"Science Faculty, Department Biology, Utrecht University , NL-3584 CH Utrecht , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3507-2808","authenticated-orcid":false,"given":"Fernanda","family":"Almeida","sequence":"additional","affiliation":[{"name":"Embrapa Amaz\u00f4nia Ocidental , 69010-97 Manaus, Amazonas , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lene","family":"Kleppe","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai Ove","family":"Skaftnesmo","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Kj\u00e6rner-Semb","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Crespo","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9237-2706","authenticated-orcid":false,"given":"Per Gunnar","family":"Fjelldal","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom Johnny","family":"Hansen","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitta","family":"Norberg","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf B","family":"Edvardsen","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Wargelius","sequence":"additional","affiliation":[{"name":"Institute of Marine Research , NO-5817 Bergen , Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2024,1,31]]},"reference":[{"issue":"3","key":"2024032909344509000_bqae013-B1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.ygcen.2009.05.004","article-title":"Control of puberty in farmed fish","volume":"165","author":"Taranger","year":"2010","journal-title":"Gen Comp Endocrinol"},{"issue":"9","key":"2024032909344509000_bqae013-B2","doi-asserted-by":"crossref","first-page":"3023","DOI":"10.1111\/are.12741","article-title":"Reduced photoperiod (18h light vs. 24h light) during first-year rearing associated with increased early male maturation in Atlantic salmon Salmo salar cultured in a freshwater recirculation aquaculture system","volume":"47","author":"Good","year":"2016","journal-title":"Aquac Res"},{"issue":"3","key":"2024032909344509000_bqae013-B3","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1016\/j.ygcen.2009.02.013","article-title":"Spermatogenesis in fish","volume":"165","author":"Schulz","year":"2010","journal-title":"Gen Comp Endocrinol"},{"issue":"3","key":"2024032909344509000_bqae013-B4","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.ando.2010.02.022","article-title":"Consequences of genetic manipulations of gonadotrophins and gonadotrophin receptors in mice","volume":"71","author":"Peltoketo","year":"2010","journal-title":"Ann Endocrinol"},{"issue":"1-2","key":"2024032909344509000_bqae013-B5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.mce.2011.08.008","article-title":"Tissue- and cell-specific functions of the androgen receptor revealed through conditional knockout models in mice","volume":"352","author":"De Gendt","year":"2012","journal-title":"Mol Cell Endocrinol"},{"issue":"1","key":"2024032909344509000_bqae013-B6","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1093\/biolre\/ioy027","article-title":"50 years of spermatogenesis: sertoli cells and their interactions with germ cells","volume":"99","author":"Griswold","year":"2018","journal-title":"Biol Reprod"},{"key":"2024032909344509000_bqae013-B7","doi-asserted-by":"crossref","first-page":"763","DOI":"10.3389\/fendo.2018.00763","article-title":"Role of follicle-stimulating hormone in spermatogenesis","volume":"9","author":"Oduwole","year":"2018","journal-title":"Front Endocrinol"},{"issue":"5","key":"2024032909344509000_bqae013-B8","doi-asserted-by":"crossref","first-page":"2349","DOI":"10.1210\/en.2009-1227","article-title":"Studies in zebrafish reveal unusual cellular expression patterns of gonadotropin receptor messenger ribonucleic acids in the testis and unexpected functional differentiation of the gonadotropins","volume":"151","author":"Garc\u00eda-L\u00f3pez","year":"2010","journal-title":"Endocrinology"},{"issue":"4","key":"2024032909344509000_bqae013-B9","doi-asserted-by":"crossref","first-page":"1427","DOI":"10.1073\/pnas.1317838111","article-title":"Germ-line activation of the luteinizing hormone receptor directly drives spermiogenesis in a nonmammalian vertebrate","volume":"111","author":"Chauvign\u00e9","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"10","key":"2024032909344509000_bqae013-B10","doi-asserted-by":"crossref","first-page":"3747","DOI":"10.1210\/en.2015-1039","article-title":"Disruption of zebrafish follicle-stimulating hormone receptor (fshr) but not luteinizing hormone receptor (lhcgr) gene by TALEN leads to failed follicle activation in females followed by sexual reversal to males","volume":"156","author":"Zhang","year":"2015","journal-title":"Endocrinology"},{"issue":"3","key":"2024032909344509000_bqae013-B11","doi-asserted-by":"crossref","first-page":"387","DOI":"10.3390\/cells11030387","article-title":"Roles of gonadotropin receptors in sexual development of Medaka","volume":"11","author":"Kitano","year":"2022","journal-title":"Cells"},{"issue":"1","key":"2024032909344509000_bqae013-B12","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/jez.1402680108","article-title":"Plasma levels of eggshell zr-proteins, estradiol-17\u03b2, and gonadotropins during an annual reproductive cycle of Atlantic salmon (Salmo salar)","volume":"268","author":"Oppen-Berntsen","year":"1994","journal-title":"J Exp Zool"},{"issue":"3","key":"2024032909344509000_bqae013-B13","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1006\/gcen.1998.7222","article-title":"Growth hormone (GH) and gonadotropin subunit gene expression and pituitary and plasma changes during spermatogenesis and oogenesis in rainbow trout (Oncorhynchus mykiss)","volume":"113","author":"Gomez","year":"1999","journal-title":"Gen Comp Endocrinol"},{"issue":"6","key":"2024032909344509000_bqae013-B14","doi-asserted-by":"crossref","first-page":"2109","DOI":"10.1095\/biolreprod.103.020560","article-title":"Endocrine changes during onset of puberty in male spring Chinook salmon, Oncorhynchus tshawytscha","volume":"69","author":"Campbell","year":"2003","journal-title":"Biol Reprod"},{"issue":"3","key":"2024032909344509000_bqae013-B15","first-page":"219","article-title":"Androgens in teleost fishes","volume":"109C","author":"Borg","year":"1994","journal-title":"Camp Biochem Physiol"},{"issue":"1","key":"2024032909344509000_bqae013-B16","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1186\/s12864-019-5869-9","article-title":"The initiation of puberty in Atlantic salmon brings about large changes in testicular gene expression that are modulated by the energy status","volume":"20","author":"Crespo","year":"2019","journal-title":"BMC Genomics"},{"issue":"11","key":"2024032909344509000_bqae013-B17","doi-asserted-by":"crossref","first-page":"e1005628","DOI":"10.1371\/journal.pgen.1005628","article-title":"The vgll3 locus controls age at maturity in wild and domesticated Atlantic salmon (Salmo salar L.) males","volume":"11","author":"Ayllon","year":"2015","journal-title":"PLoS Genet"},{"issue":"7582","key":"2024032909344509000_bqae013-B18","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1038\/nature16062","article-title":"Sex-dependent dominance at a single locus maintains variation in age at maturity in salmon","volume":"528","author":"Barson","year":"2015","journal-title":"Nature"},{"issue":"3","key":"2024032909344509000_bqae013-B19","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1530\/JOE-13-0240","article-title":"Salinity and photoperiod modulate pubertal development in Atlantic salmon (Salmo salar)","volume":"220","author":"Melo","year":"2014","journal-title":"J Endocrinol"},{"key":"2024032909344509000_bqae013-B20","doi-asserted-by":"crossref","first-page":"738877","DOI":"10.1016\/j.aquaculture.2022.738877","article-title":"Interaction of temperature and feed ration on male postsmolt maturation of Atlantic salmon (Salmo salar L.)","volume":"562","author":"Martinez E","year":"2023","journal-title":"Aquaculture"},{"key":"2024032909344509000_bqae013-B21","doi-asserted-by":"crossref","first-page":"739325","DOI":"10.1016\/j.aquaculture.2023.739325","article-title":"Interaction of temperature and photoperiod on male postsmolt maturation of Atlantic salmon (Salmo salar L.)","volume":"568","author":"Martinez E","year":"2023","journal-title":"Aquaculture"},{"issue":"7","key":"2024032909344509000_bqae013-B22","doi-asserted-by":"crossref","first-page":"e68708","DOI":"10.1371\/journal.pone.0068708","article-title":"Heritable and precise zebrafish genome editing using a CRISPR-cas system","volume":"8","author":"Hwang","year":"2013","journal-title":"PLoS One"},{"issue":"5","key":"2024032909344509000_bqae013-B23","doi-asserted-by":"crossref","first-page":"e98186","DOI":"10.1371\/journal.pone.0098186","article-title":"Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs","volume":"9","author":"Gagnon","year":"2014","journal-title":"PloS One"},{"issue":"9","key":"2024032909344509000_bqae013-B24","doi-asserted-by":"crossref","first-page":"e108622","DOI":"10.1371\/journal.pone.0108622","article-title":"Targeted mutagenesis in Atlantic salmon (Salmo salar L.) using the CRISPR\/Cas9 system induces complete knockout individuals in the F0 generation","volume":"9","author":"Edvardsen","year":"2014","journal-title":"PLoS One"},{"issue":"1","key":"2024032909344509000_bqae013-B25","doi-asserted-by":"crossref","first-page":"21284","DOI":"10.1038\/srep21284","article-title":"Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon","volume":"6","author":"Wargelius","year":"2016","journal-title":"Sci Rep"},{"issue":"1","key":"2024032909344509000_bqae013-B26","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/s12863-019-0745-9","article-title":"The influence of vgll3 genotypes on sea age at maturity is altered in farmed mowi strain Atlantic salmon","volume":"20","author":"Ayllon","year":"2019","journal-title":"BMC Genet"},{"issue":"1-2","key":"2024032909344509000_bqae013-B27","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.aquaculture.2011.08.017","article-title":"Continuous light and elevated temperature can trigger maturation both during and immediately after smoltification in male Atlantic salmon (Salmo salar)","volume":"321","author":"Fjelldal","year":"2011","journal-title":"Aquaculture"},{"key":"2024032909344509000_bqae013-B28","author":"Andersson"},{"issue":"1","key":"2024032909344509000_bqae013-B29","doi-asserted-by":"crossref","first-page":"12584","DOI":"10.1038\/s41598-017-12936-w","article-title":"Sex steroid production associated with puberty is absent in germ cell-free salmon","volume":"7","author":"Kleppe","year":"2017","journal-title":"Sci Rep"},{"issue":"1","key":"2024032909344509000_bqae013-B30","doi-asserted-by":"crossref","first-page":"18042","DOI":"10.1038\/s41598-020-74876-2","article-title":"Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon","volume":"10","author":"G\u00fcralp","year":"2020","journal-title":"Sci Rep"},{"issue":"5","key":"2024032909344509000_bqae013-B31","doi-asserted-by":"crossref","first-page":"1792","DOI":"10.1093\/nar\/gkh340","article-title":"MUSCLE: multiple sequence alignment with high accuracy and high throughput","volume":"32","author":"Edgar","year":"2004","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"2024032909344509000_bqae013-B32","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/1471-2199-6-21","article-title":"Evaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmon","volume":"6","author":"Olsvik","year":"2005","journal-title":"BMC Mol Biol"},{"key":"2024032909344509000_bqae013-B33","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.ygcen.2013.07.001","article-title":"Pituitary gonadotropin and ovarian gonadotropin receptor transcript levels: seasonal and photoperiod-induced changes in the reproductive physiology of female Atlantic salmon (Salmo salar)","volume":"191","author":"Andersson","year":"2013","journal-title":"Gen Comp Endocrinol"},{"issue":"4","key":"2024032909344509000_bqae013-B34","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods San Diego Calif"},{"issue":"2","key":"2024032909344509000_bqae013-B35","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/1367-8280(94)90035-3","article-title":"Enzyme immunoassay for 11-ketotestosterone using acetylcholinesterase as laberl: application to the measurement of 11-ketotestosterone in plasma of Siberian sturgeon","volume":"108","author":"Cuisset","year":"1994","journal-title":"Comp Biochem Physiol C Pharmacol Toxicol Endocrinol"},{"issue":"7602","key":"2024032909344509000_bqae013-B36","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1038\/nature17164","article-title":"The Atlantic salmon genome provides insights into rediploidization","volume":"533","author":"Lien","year":"2016","journal-title":"Nature"},{"issue":"1","key":"2024032909344509000_bqae013-B37","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/s12864-020-6513-4","article-title":"Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon","volume":"21","author":"Kleppe","year":"2020","journal-title":"BMC Genomics"},{"key":"2024032909344509000_bqae013-B38","doi-asserted-by":"crossref","first-page":"977779","DOI":"10.3389\/fcell.2022.977779","article-title":"The Piwil1 N domain is required for germ cell survival in Atlantic salmon","volume":"10","author":"Almeida","year":"2022","journal-title":"Front Cell Dev Biol"},{"key":"2024032909344509000_bqae013-B39","doi-asserted-by":"crossref","first-page":"707","DOI":"10.3389\/fendo.2018.00707","article-title":"Structure-function relationships of the follicle-stimulating hormone receptor","volume":"9","author":"Ulloa-Aguirre","year":"2018","journal-title":"Front Endocrinol"},{"key":"2024032909344509000_bqae013-B40","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/B978-0-12-417047-6.00013-2","volume-title":"Sertoli Cell Biology","author":"Fran\u00e7a","year":"2015"},{"key":"2024032909344509000_bqae013-B41","doi-asserted-by":"crossref","first-page":"113244","DOI":"10.1016\/j.ygcen.2019.113244","article-title":"Regulation of spermatogonial development by Fsh: the complementary roles of locally produced Igf and Wnt signaling molecules in adult zebrafish testis","volume":"284","author":"Safian","year":"2019","journal-title":"Gen Comp Endocrinol"},{"issue":"46","key":"2024032909344509000_bqae013-B42","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2209353119","article-title":"Neuropeptide FF indirectly affects testicular morphogenesis and functions in medaka","volume":"119","author":"Tomihara","year":"2022","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"1","key":"2024032909344509000_bqae013-B43","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1210\/me.2014-1256","article-title":"Genetic analysis of zebrafish gonadotropin (FSH and LH) functions by TALEN-mediated gene disruption","volume":"29","author":"Zhang","year":"2015","journal-title":"Mol Endocrinol Baltim Md"},{"key":"2024032909344509000_bqae013-B44","doi-asserted-by":"crossref","first-page":"113276","DOI":"10.1016\/j.ygcen.2019.113276","article-title":"Characterization of gonadotropin receptors Fshr and Lhr in Japanese medaka, Oryzias latipes","volume":"285","author":"Burow","year":"2020","journal-title":"Gen Comp Endocrinol"},{"issue":"1","key":"2024032909344509000_bqae013-B45","doi-asserted-by":"crossref","first-page":"1428","DOI":"10.1038\/s41467-023-37026-6","article-title":"Evolutionary differentiation of androgen receptor is responsible for sexual characteristic development in a teleost fish","volume":"14","author":"Ogino","year":"2023","journal-title":"Nat Commun"},{"issue":"3","key":"2024032909344509000_bqae013-B46","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.ygcen.2009.05.001","article-title":"Pharmacological characterization, localization and quantification of expression of gonadotropin receptors in Atlantic salmon (Salmo salar L.) ovaries","volume":"163","author":"Andersson","year":"2009","journal-title":"Gen Comp Endocrinol"},{"issue":"6","key":"2024032909344509000_bqae013-B47","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1095\/biolreprod.107.062299","article-title":"Follicle-stimulating hormone induces spermatogenesis mediated by androgen production in Japanese eel, Anguilla japonica","volume":"77","author":"Ohta","year":"2007","journal-title":"Biol Reprod"},{"issue":"1","key":"2024032909344509000_bqae013-B48","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1210\/en.2008-0447","article-title":"Leydig cells express follicle-stimulating hormone receptors in African catfish","volume":"150","author":"Garc\u00eda-L\u00f3pez","year":"2009","journal-title":"Endocrinology"},{"issue":"3","key":"2024032909344509000_bqae013-B49","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1095\/biolreprod52.3.697","article-title":"Maturation-associated changes in the response of the salmon testis to the steroidogenic actions of gonadotropins (GTH I and GTH II) in vitro","volume":"52","author":"Planas","year":"1995","journal-title":"Biol Reprod"},{"issue":"9","key":"2024032909344509000_bqae013-B50","doi-asserted-by":"crossref","first-page":"3527","DOI":"10.1210\/en.2010-1469","article-title":"Proteolytically activated, recombinant Anti-M\u00fcllerian hormone inhibits androgen secretion, proliferation, and differentiation of spermatogonia in adult zebrafish testis organ cultures","volume":"152","author":"Skaar","year":"2011","journal-title":"Endocrinology"},{"issue":"1","key":"2024032909344509000_bqae013-B51","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.ydbio.2006.10.001","article-title":"A novel transforming growth factor-beta superfamily member expressed in gonadal somatic cells enhances primordial germ cell and spermatogonial proliferation in rainbow trout (Oncorhynchus mykiss)","volume":"301","author":"Sawatari","year":"2007","journal-title":"Dev Biol"},{"issue":"21","key":"2024032909344509000_bqae013-B52","first-page":"dev178665","article-title":"Endocrine and local signaling interact to regulate spermatogenesis in zebrafish: follicle-stimulating hormone, retinoic acid and androgens","volume":"146","author":"Crespo","year":"2019","journal-title":"Dev Camb Engl"},{"issue":"1","key":"2024032909344509000_bqae013-B53","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1530\/JOE-19-0309","article-title":"PGE2 inhibits spermatogonia differentiation in zebrafish: interaction with Fsh and an androgen","volume":"244","author":"Crespo","year":"2020","journal-title":"J Endocrinol"},{"issue":"6","key":"2024032909344509000_bqae013-B54","first-page":"782","article-title":"Differential recruitment of steroid hormone response elements may dictate the expression of the pituitary gonadotropin II beta subunit gene during salmon maturation","volume":"8","author":"Xiong","year":"1994","journal-title":"Mol Endocrinol Baltim Md"},{"key":"2024032909344509000_bqae013-B55","doi-asserted-by":"crossref","first-page":"826920","DOI":"10.3389\/fendo.2022.826920","article-title":"Pituitary gonadotropin gene expression during induced onset of postsmolt maturation in male atlantic salmon: in vivo and tissue culture studies","volume":"13","author":"Crespo","year":"2022","journal-title":"Front Endocrinol"},{"key":"2024032909344509000_bqae013-B56","doi-asserted-by":"crossref","first-page":"738456","DOI":"10.1016\/j.aquaculture.2022.738456","article-title":"Full production cycle performance of gene-edited, sterile Atlantic salmon\u2014growth, smoltification, welfare indicators and fillet composition","volume":"560","author":"Kleppe","year":"2022","journal-title":"Aquaculture"},{"issue":"2","key":"2024032909344509000_bqae013-B57","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1093\/biolre\/ioy204","article-title":"Novel method for mass producing genetically sterile fish from surrogate broodstock via spermatogonial transplantation\u2020","volume":"100","author":"Nagasawa","year":"2019","journal-title":"Biol Reprod"}],"container-title":["Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/endo\/advance-article-pdf\/doi\/10.1210\/endocr\/bqae013\/56528442\/bqae013.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/endo\/article-pdf\/165\/4\/bqae013\/57108159\/bqae013.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/endo\/article-pdf\/165\/4\/bqae013\/57108159\/bqae013.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T09:35:36Z","timestamp":1711704936000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/endo\/article\/doi\/10.1210\/endocr\/bqae013\/7595588"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,31]]},"references-count":57,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,2,20]]}},"URL":"https:\/\/doi.org\/10.1210\/endocr\/bqae013","relation":{},"ISSN":["1945-7170"],"issn-type":[{"value":"1945-7170","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,4]]},"published":{"date-parts":[[2024,1,31]]},"article-number":"bqae013"}}