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This concept is derived from earlier studies of Drosophila, which has been confirmed in this and earlier reports as well. Herein, we use the approach of knockdown of Fat1 by RNAi using primary cultures of Sertoli cells that mimicked the blood\u2013testis barrier (BTB) in vivo, and a series of coherent experiments including functional assays to monitor the Sertoli cell tight junction (TJ) permeability barrier and a functional in vitro TJ integrity assay to assess the role of Fat1 in the testis. It was shown that planar cell polarity (PCP) protein Fat1 affected Sertoli cell function through its modulation of actin and microtubule cytoskeletal function, altering their polymerization activity through the Fat1\/Fjx1 complex. Furthermore, Fat1 is intimately associated with \u03b2-catenin and \u03b1-N-catenin, as well as with Prickle 1 of the Vangl1\/Prickle 1 complex, another PCP core protein to support intercellular interactions to confer PCP. In summary, these findings support the notion that the Fat:Dchs and the Vangl2:Fzd PCP intercellular bridges are tightly associated with basal ES\/TJ structural proteins to stabilize PCP function at the Sertoli:Sertoli, Sertoli:spermatid, and spermatid:spermatid interface to sustain spermatogenesis.<\/jats:p>","DOI":"10.1210\/endocr\/bqae041","type":"journal-article","created":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T07:03:08Z","timestamp":1711782188000},"source":"Crossref","is-referenced-by-count":3,"title":["The Planar Cell Polarity Protein Fat1 in Sertoli Cell Function"],"prefix":"10.1210","volume":"165","author":[{"given":"Tiao","family":"Bu","sequence":"first","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School of Nantong University , Nantong, Jiangsu 226001 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingling","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School of Nantong University , Nantong, Jiangsu 226001 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3050-0324","authenticated-orcid":false,"given":"Xiaolong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Urology and Andrology, Sir Run-Run Shaw Hospital, Zhejiang University School of Medicine , Hangzhou, Zhejiang 310016 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School of Nantong University , Nantong, Jiangsu 226001 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyao","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Medicine, Medical School of Nantong University , Nantong, Jiangsu 226001 , 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