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In this study, we aimed to identify whether \u03b2-adrenergic signaling has a direct effect on PVAT. Thoracic aorta PVAT was obtained from male Wistar rats and cultured ex vivo with the \u03b2-AR agonist isoproterenol (Iso; 1\u2005\u00b5M) or vehicle for 24 hours. Conditioned culture medium (CCM) from Iso-treated PVAT induced a marked increase in aorta contractile response, induced oxidative stress, and reduced nitric oxide production in PVAT compared to vehicle. In addition, Iso-treated PVAT and PVAT-derived differentiated adipocytes exhibited higher corticosterone release and protein expression of 11\u03b2\u2013hydroxysteroid dehydrogenase type 1 (11\u03b2-HSD1), an enzyme responsible for de novo synthesis of corticosterone. Macrophages exposed to Iso also exhibited increased corticosterone release in response to \u03b2-AR stimulation. Incubation of Iso-treated PVAT and PVAT-derived differentiated adipocytes with \u03b23-AR antagonist restored aorta contractile function modulated by Iso-CCM and normalized 11\u03b2-HSD1 protein expression. These results show that \u03b23-AR signaling leads to upregulation of 11\u03b2-HSD1 in PVAT, thus increasing corticosterone release and contributing to impair the anticontractile function of this tissue.<\/jats:p>","DOI":"10.1210\/endocr\/bqae053","type":"journal-article","created":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T15:58:27Z","timestamp":1714665507000},"source":"Crossref","is-referenced-by-count":3,"title":["\u03b2-Adrenergic Stimulation-Induced PVAT Dysfunction in Male Sex: A Role for 11\u03b2-Hydroxysteroid Dehydrogenase-1"],"prefix":"10.1210","volume":"165","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0686-5844","authenticated-orcid":false,"given":"Jamaira Aparecida","family":"Victorio","sequence":"first","affiliation":[{"name":"Laboratory of Vascular Biology (LaBiVasc), Department of Structural and Functional Biology, Universidade Estadual de Campinas (UNICAMP) , S\u00e3o Paulo 13083\u2013865 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Comorbidities Research Center, Universidade Estadual de Campinas (UNICAMP) , S\u00e3o Paulo 13083\u2013865 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana Venturini","family":"Rossoni","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo , Sao Paulo 05508-000 ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9862-4262","authenticated-orcid":false,"given":"Ana Paula","family":"Davel","sequence":"additional","affiliation":[{"name":"Laboratory of Vascular Biology (LaBiVasc), Department of Structural and Functional Biology, Universidade Estadual de Campinas (UNICAMP) , S\u00e3o Paulo 13083\u2013865 ,","place":["Brazil"]},{"name":"Obesity and Comorbidities Research Center, Universidade Estadual de Campinas (UNICAMP) , S\u00e3o Paulo 13083\u2013865 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