{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:43:33Z","timestamp":1792453413806,"version":"4.5.0"},"reference-count":109,"publisher":"The Endocrine Society","issue":"2","license":[{"start":{"date-parts":[[2025,1,6]],"date-time":"2025-01-06T00:00:00Z","timestamp":1736121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["HD071875"],"award-info":[{"award-number":["HD071875"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["HD094296"],"award-info":[{"award-number":["HD094296"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study uncovers a dynamic shift in estrogen receptor expression during granulosa cell (GC) differentiation in the ovary, highlighting a transition from estrogen receptor alpha (ESR1) to estrogen receptor beta (ESR2). Using a transgenic mouse model with Esr1-iCre-mediated Esr2 deletion, we demonstrate that ESR2 expression is absent in GCs derived from ESR1-expressing ovarian surface epithelium (OSE) cells. Single-cell analysis of the OSE-GC lineage reveals a developmental trajectory from Esr1-expressing OSE cells to Foxl2-expressing pre-GCs, culminating in GCs exclusively expressing Esr2. Transcriptome analyses identified vasculature-derived TGF\u03b21 ligands as key regulators of this transition. Supporting this, TGF\u03b21 treatment of cultured embryonic ovaries reduced Esr1 expression while promoting Esr2 expression. This study underscores the capability of GCs to switch from ESR1 to ESR2 expression as a fundamental aspect of normal differentiation.<\/jats:p>","DOI":"10.1210\/endocr\/bqaf006","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T08:13:57Z","timestamp":1737447237000},"source":"Crossref","is-referenced-by-count":7,"title":["A Dynamic Shift in Estrogen Receptor Expression During Granulosa Cell Differentiation in the Ovary"],"prefix":"10.1210","volume":"166","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9008-1964","authenticated-orcid":false,"given":"Chan Jin","family":"Park","sequence":"first","affiliation":[{"name":"Department of Comparative Biosciences, College of Veterinary Medicine, University of Illinois at Urbana-Champaign , Urbana, IL 61802 ,","place":["USA"]},{"name":"Epivara, Inc., Research Park , Champaign, IL 61820 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