{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T10:14:55Z","timestamp":1778148895900,"version":"3.51.4"},"reference-count":49,"publisher":"The Endocrine Society","issue":"5","license":[{"start":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T00:00:00Z","timestamp":1775520000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"MIPM"},{"DOI":"10.13039\/501100004189","name":"Max Planck Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004189","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001655","name":"DAAD","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001655","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The adenohypophyseal placode is a developmental structure that is conserved among vertebrates and gives rise to the anterior pituitary. The earliest steps of its formation remain poorly understood. Here, we present a powerful new genetic tool: a zebrafish GAL4 knock-in reporter line for prop1. The targeted integration disrupts prop1 function, generating the first prop1\u2212\/\u2212 zebrafish, which exhibits dwarfism, impaired sexual maturation, and pigmentation defects. The generation of this new reporter line enabled the study of early pituitary development at single-cell resolution, using both scRNA-seq and live imaging. We identified epcam\/Epcam as a conserved epithelial marker of the adenohypophysis in both zebrafish and mouse. We further studied transcriptional programs underlying endocrine lineage specification, discovering several previously unrecognized markers for hormone-producing lineages, including tfcp2l1, as a likely regulator of lactotrope differentiation. Cell-cell interaction analyses indicated Notch, Fgf, and Slit\/Robo signaling as key regulators of progenitor maintenance and differentiation. Overall, this work provides the first single-cell atlas of early zebrafish pituitary development, a robust new genetic tool to study fundamental mechanistic insights into pituitary lineage specification and differentiation.<\/jats:p>","DOI":"10.1210\/endocr\/bqag043","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T11:27:24Z","timestamp":1776770844000},"source":"Crossref","is-referenced-by-count":0,"title":["Generation of a\n                    <i>prop1<\/i>\n                    knock-in zebrafish enables single-cell transcriptomics of early pituitary development"],"prefix":"10.1210","volume":"167","author":[{"given":"Julian","family":"Martinez-Mayer","sequence":"first","affiliation":[{"name":"Instituto de Fisiolog\u00eda, Biolog\u00eda Molecular y Neurociencias (IFIBYNE), UBA CONICET , Buenos Aires C1428EHA ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mridula","family":"Balakrishnan","sequence":"additional","affiliation":[{"name":"Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research , Bad Nauheim 61231 ,","place":["Germany"]},{"name":"German Centre for Cardiovascular Research (Deutsches Zentrum f\u00fcr Herz-Kreislaufforschung) , Bad Nauheim 61231 ,","place":["Germany"]},{"name":"Cardio-Pulmonary Institute , Bad Nauheim 61231 ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard Y","family":"Cheung","sequence":"additional","affiliation":[{"name":"Department of Physiology & Biophysics, Renaissance School of Medicine at Stony Brook University , Stony Brook, NY 1794-8661 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5594-4549","authenticated-orcid":false,"given":"Stefan","family":"G\u00fcnther","sequence":"additional","affiliation":[{"name":"Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research , Bad Nauheim 61231 ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Violeta","family":"Medan","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda, Biolog\u00eda Molecular y Neurociencias (IFIBYNE), UBA CONICET , Buenos Aires C1428EHA ,","place":["Argentina"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Didier Y R","family":"Stainier","sequence":"additional","affiliation":[{"name":"Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research , Bad Nauheim 61231 ,","place":["Germany"]},{"name":"German Centre for Cardiovascular Research (Deutsches Zentrum 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