{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:12:52Z","timestamp":1790874772607,"version":"4.1.0"},"reference-count":33,"publisher":"The Endocrine Society","issue":"7","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009633","name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["R37 HD014939"],"award-info":[{"award-number":["R37 HD014939"]}],"id":[{"id":"10.13039\/100009633","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012756","name":"Lalor Foundation","doi-asserted-by":"publisher","award":["AG20240665"],"award-info":[{"award-number":["AG20240665"]}],"id":[{"id":"10.13039\/100012756","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>To facilitate the investigation of signaling by the luteinizing hormone receptor (LHR), we created a mouse line called Lhr-COIN. This line allows for the conditional replacement of the Lhr coding sequence with enhanced green fluorescent protein (eGFP), resulting in both a conditional knockout line and a reporter line. By breeding these mice with mice expressing Cre recombinase, we generated mice in which either one or both Lhr alleles were replaced with eGFP. Notably, mice in which one Lhr allele in the granulosa cells was replaced with eGFP exhibited normal LH responsiveness. This enabled live imaging of LH-induced migration of LH-receptor-expressing granulosa cells within preovulatory ovarian follicles. The Lhr-COIN mouse line holds significant potential for future research on LHR function and localization in the ovary and other tissues.<\/jats:p>","DOI":"10.1210\/endocr\/bqag065","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T11:42:00Z","timestamp":1780141320000},"source":"Crossref","is-referenced-by-count":1,"title":["Conditional replacement of the mouse luteinizing hormone receptor with green fluorescent protein, enabling imaging of cell migration during ovulation"],"prefix":"10.1210","volume":"167","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7409-9259","authenticated-orcid":false,"given":"Corie M","family":"Owen","sequence":"first","affiliation":[{"name":"Department of Cell Biology, University of Connecticut Health Center , Farmington, CT 06030 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7649-974X","authenticated-orcid":false,"given":"Katie M","family":"Lowther","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Connecticut Health Center , Farmington, CT 06030 ,","place":["USA"]},{"name":"Center for Mouse Genome Modification, University of Connecticut Health Center , Farmington, CT 06030 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Kaback","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Connecticut Health Center , Farmington, CT 06030 ,","place":["USA"]},{"name":"Center for Mouse Genome Modification, University of Connecticut Health Center , Farmington, CT 06030 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2636-5721","authenticated-orcid":false,"given":"Laurinda A","family":"Jaffe","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Connecticut Health Center , Farmington, CT 06030 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2427-401X","authenticated-orcid":false,"given":"Siu-Pok","family":"Yee","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Connecticut Health Center , Farmington, CT 06030 ,","place":["USA"]},{"name":"Center for Mouse Genome Modification, University of Connecticut Health Center , Farmington, CT 06030 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2026,6,2]]},"reference":[{"key":"2026061613331000000_bqag065-B1","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/B978-0-12-397175-3.00020-X","volume-title":"Knobil and Neill\u2019s Physiology of Reproduction","author":"Hunzicker-Dunn","year":"2015"},{"key":"2026061613331000000_bqag065-B2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/978-3-031-96376-6_8","volume-title":"Leydig Cells","author":"Rivero-M\u00fcller","year":"2025"},{"issue":"8","key":"2026061613331000000_bqag065-B3","doi-asserted-by":"crossref","first-page":"3548","DOI":"10.3390\/ijms26083548","article-title":"Luteinizing hormone regulates testosterone production, Leydig cell proliferation, differentiation, and circadian rhythm during spermatogenesis","volume":"26","author":"Lei","year":"2025","journal-title":"Int J Mol Sci"},{"issue":"4","key":"2026061613331000000_bqag065-B4","first-page":"686","article-title":"Evolution of gonadotropin signaling on gonad development: insights from gene knockout studies in zebrafish","volume":"99","author":"Li","year":"2018","journal-title":"Biol Reprod"},{"key":"2026061613331000000_bqag065-B5","doi-asserted-by":"crossref","DOI":"10.1016\/bs.ctdb.2026.04.008","article-title":"Intercellular cyclic nucleotide dynamics mediate oocyte meiosis in mammalian preovulatory follicles","volume":"169:193-235","author":"Egbert","year":"2026","journal-title":"Curr Top Dev Biol"},{"key":"2026061613331000000_bqag065-B6","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/bs.ctdb.2024.10.011","article-title":"Luteinizing hormone-induced changes in the structure of mammalian preovulatory follicles","volume":"162","author":"Owen","year":"2025","journal-title":"Curr Top Dev Biol"},{"issue":"5","key":"2026061613331000000_bqag065-B7","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.tem.2018.02.012","article-title":"Endocrine, paracrine, and autocrine signaling pathways that regulate ovulation","volume":"29","author":"Richards","year":"2018","journal-title":"Trends Endocrinol Metab"},{"issue":"11","key":"2026061613331000000_bqag065-B8","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1038\/s41556-024-01524-6","article-title":"Ex vivo imaging reveals the spatiotemporal control of ovulation","volume":"26","author":"Thomas","year":"2024","journal-title":"Nat Cell Biol"},{"issue":"1","key":"2026061613331000000_bqag065-B9","doi-asserted-by":"crossref","first-page":"108042","DOI":"10.1016\/j.jbc.2024.108042","article-title":"Metabolic control of luteinizing hormone-responsive ovarian steroidogenesis","volume":"301","author":"Przygrodzka","year":"2025","journal-title":"J Biol Chem"},{"issue":"7","key":"2026061613331000000_bqag065-B10","doi-asserted-by":"crossref","DOI":"10.1210\/endocr\/bqaa074","article-title":"Cellular heterogeneity of the luteinizing hormone receptor and its significance for cyclic GMP signaling in mouse preovulatory follicles","volume":"161","author":"Baena","year":"2020","journal-title":"Endocrinology"},{"issue":"1","key":"2026061613331000000_bqag065-B11","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1210\/mend.15.1.0586","article-title":"Targeted disruption of luteinizing hormone\/human chorionic gonadotropin receptor gene","volume":"15","author":"Lei","year":"2001","journal-title":"Mol Endocrinol"},{"issue":"1","key":"2026061613331000000_bqag065-B12","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1210\/mend.15.1.0582","article-title":"Normal prenatal but arrested postnatal sexual development of luteinizing hormone receptor knockout (LuRKO) mice","volume":"15","author":"Zhang","year":"2001","journal-title":"Mol Endocrinol"},{"issue":"2","key":"2026061613331000000_bqag065-B13","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1093\/biolre\/ioad142","article-title":"Luteinizing hormone stimulates ingression of mural granulosa cells within the mouse preovulatory follicle","volume":"110","author":"Owen","year":"2024","journal-title":"Biol Reprod"},{"issue":"9","key":"2026061613331000000_bqag065-B14","doi-asserted-by":"crossref","first-page":"2443","DOI":"10.1016\/j.celrep.2019.07.088","article-title":"The ovulatory signal precipitates LRH-1 transcriptional switching mediated by differential chromatin accessibility","volume":"28","author":"Bianco","year":"2019","journal-title":"Cell Rep"},{"issue":"4","key":"2026061613331000000_bqag065-B15","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1111\/andr.70000","article-title":"Luteinizing hormone receptor knockout mouse: what has it taught US?","volume":"14","author":"Huhtaniemi","year":"2026","journal-title":"Andrology"},{"issue":"17","key":"2026061613331000000_bqag065-B16","doi-asserted-by":"crossref","DOI":"10.15252\/embj.201898984","article-title":"Luteinizing hormone signaling restricts hematopoietic stem cell expansion during puberty","volume":"37","author":"Peng","year":"2018","journal-title":"EMBO J"},{"key":"2026061613331000000_bqag065-B17","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.79612","article-title":"Brain atlas for glycoprotein hormone receptors at single-transcript level","volume":"11","author":"Ryu","year":"2022","journal-title":"eLife"},{"issue":"1","key":"2026061613331000000_bqag065-B18","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1093\/biolreprod\/64.1.179","article-title":"Expression and localization of luteinizing hormone receptor in the female mouse reproductive tract","volume":"64","author":"Zhang","year":"2001","journal-title":"Biol Reprod"},{"issue":"5","key":"2026061613331000000_bqag065-B19","doi-asserted-by":"crossref","DOI":"10.1210\/endocr\/bqab035","article-title":"The luteinizing hormone receptor knockout mouse as a tool to probe the in vivo actions of gonadotropic hormones\/receptors in females","volume":"162","author":"Jonas","year":"2021","journal-title":"Endocrinology"},{"key":"2026061613331000000_bqag065-B20","doi-asserted-by":"crossref","unstructured":"Owen CM, Lowther K, Kaback D, Jaffe LA, Yee SP. Supplemental data for \u201cConditional replacement of the mouse luteinizing hormone receptor with green fluorescent protein, enabling imaging of cell migration during ovulation\u201d. Figshare. 2026. 10.6084\/m9.figshare.32030100.v1","DOI":"10.64898\/2026.05.21.726840"},{"issue":"3","key":"2026061613331000000_bqag065-B21","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1002\/gene.10030","article-title":"A Cre\/loxP-deleter transgenic line in mouse strain 129S1\/SvImJ","volume":"32","author":"Tang","year":"2002","journal-title":"Genesis"},{"issue":"4","key":"2026061613331000000_bqag065-B22","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.celrep.2014.12.040","article-title":"Medial amygdalar aromatase neurons regulate aggression in both sexes","volume":"10","author":"Unger","year":"2015","journal-title":"Cell Rep"},{"issue":"19","key":"2026061613331000000_bqag065-B23","doi-asserted-by":"crossref","first-page":"3229","DOI":"10.1242\/dev.025494","article-title":"Luteinizing hormone causes MAP kinase-dependent phosphorylation and closure of connexin 43 gap junctions in mouse ovarian follicles: one of two paths to meiotic resumption","volume":"135","author":"Norris","year":"2008","journal-title":"Development"},{"issue":"5","key":"2026061613331000000_bqag065-B24","doi-asserted-by":"crossref","first-page":"2142","DOI":"10.1210\/en.2018-00017","article-title":"Multiple cAMP phosphodiesterases act together to prevent premature oocyte meiosis and ovulation","volume":"159","author":"Vigone","year":"2018","journal-title":"Endocrinology"},{"issue":"49","key":"2026061613331000000_bqag065-B25","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2524136122","article-title":"Intercellular diffusion of cyclic nucleotides followed by gap junction closure restarts meiosis in mouse preovulatory follicles","volume":"122","author":"Nakashima","year":"2025","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"2026061613331000000_bqag065-B26","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji\u2014an open source platform for biological image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat Methods"},{"issue":"6","key":"2026061613331000000_bqag065-B27","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1016\/j.celrep.2014.05.031","article-title":"A conditional system to specifically link disruption of protein-coding function with reporter expression in mice","volume":"7","author":"Chiou","year":"2014","journal-title":"Cell Rep"},{"issue":"34","key":"2026061613331000000_bqag065-B28","doi-asserted-by":"crossref","first-page":"E3179","DOI":"10.1073\/pnas.1217812110","article-title":"Conditionals by inversion provide a universal method for the generation of conditional alleles","volume":"110","author":"Economides","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"7","key":"2026061613331000000_bqag065-B29","doi-asserted-by":"crossref","first-page":"1862","DOI":"10.1172\/JCI24562","article-title":"Fertility in luteinizing hormone receptor-knockout mice after wild-type ovary transplantation demonstrates redundancy of extragonadal luteinizing hormone action","volume":"115","author":"Pakarainen","year":"2005","journal-title":"J Clin Invest"},{"issue":"6","key":"2026061613331000000_bqag065-B30","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1093\/biolre\/ioab067","article-title":"Kisspeptin-54 injection induces a physiological luteinizing hormone surge and ovulation in mice","volume":"104","author":"Owen","year":"2021","journal-title":"Biol Reprod"},{"issue":"1","key":"2026061613331000000_bqag065-B31","doi-asserted-by":"crossref","first-page":"9731","DOI":"10.1038\/s41598-019-46282-w","article-title":"Actin stabilizing compounds show specific biological effects due to their binding mode","volume":"9","author":"Wang","year":"2019","journal-title":"Sci Rep"},{"key":"2026061613331000000_bqag065-B32","doi-asserted-by":"crossref","first-page":"896994","DOI":"10.3389\/fphar.2022.896994","article-title":"Synthesis, screening and characterization of novel potent Arp2\/3 inhibitory compounds analogous to CK-666","volume":"13","author":"Fokin","year":"2022","journal-title":"Front Pharmacol"},{"issue":"12","key":"2026061613331000000_bqag065-B33","doi-asserted-by":"crossref","first-page":"4794","DOI":"10.1210\/en.2016-1351","article-title":"Pulse and surge profiles of luteinizing hormone secretion in the mouse","volume":"157","author":"Czieselsky","year":"2016","journal-title":"Endocrinology"}],"container-title":["Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/endo\/advance-article-pdf\/doi\/10.1210\/endocr\/bqag065\/68456589\/bqag065.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/endo\/article-pdf\/167\/7\/bqag065\/68456589\/bqag065.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/endo\/article-pdf\/167\/7\/bqag065\/68456589\/bqag065.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:33:22Z","timestamp":1781631202000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/endo\/article\/doi\/10.1210\/endocr\/bqag065\/8700230"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,26]]},"references-count":33,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,5,26]]}},"URL":"https:\/\/doi.org\/10.1210\/endocr\/bqag065","relation":{},"ISSN":["1945-7170"],"issn-type":[{"value":"1945-7170","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026,7]]},"published":{"date-parts":[[2026,5,26]]},"article-number":"bqag065"}}