{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:05:02Z","timestamp":1785769502586,"version":"3.56.0"},"reference-count":96,"publisher":"The Endocrine Society","issue":"9","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"John P. Hughes Endowment at the University of California, Davis"},{"DOI":"10.13039\/100023065","name":"State of California","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100023065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institute of Health","doi-asserted-by":"publisher","award":["T32OD011147"],"award-info":[{"award-number":["T32OD011147"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Kenneth Burns Endowed Chair in Veterinary Medicine at the Louisiana State University School of Veterinary Medicine","award":["2032-10700-003-000-D"],"award-info":[{"award-number":["2032-10700-003-000-D"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The endocrinology of equine pregnancy is unique, but many of its unusual phenomena closely resemble pregnancy in women. Placental estrone synthesis uses fetal adrenal androgen precursors in women, but from the fetal gonads in mares, peaking midway through the 11-month gestation. Unique to equine pregnancies, other estrogens like equilin, typified by an unsaturated B-ring sterol structure, are synthesized from 7-dehydrocholesterol (7-DHC), the immediate precursor to cholesterol, without cholesterol formation, peaking later in gestation. Currently, the spatial and temporal regulation underlying the redirection of 7-DHC from cholesterol to B-ring unsaturated steroid synthesis in equine fetal gonads remains unknown. Here, we investigate the developmental dynamics of the equine fetal gonads from the fourth to eleventh gestational month using RNA sequencing, immunofluorescence imaging, RNAScope, and gas chromatography\u2013mass spectrometry. Our results suggest that placental estrone synthesis correlates with the expression of the last enzyme in the cholesterol synthesis pathway, 7-dehydrocholesterol reductase (DHCR7) in the fetal gonad, and its downregulation may drive the accumulation of 7-DHC and the synthesis of B-ring unsaturated steroids. B-ring unsaturated steroid secretion is the hallmark of Smith\u2013Lemli\u2013Opitz Syndrome in humans, resulting from mutations in the gene encoding DHCR7 in affected patients. This naturally occurring phenomenon in equine pregnancies may offer a unique comparative model for understanding the metabolic consequences of 7-DHC accumulation in these patients and a platform for drug development.<\/jats:p>","DOI":"10.1210\/endocr\/bqag083","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:42:01Z","timestamp":1784893321000},"source":"Crossref","is-referenced-by-count":1,"title":["Compartmentalization of DHCR7 links 7-DHC metabolism to B-ring unsaturated steroid synthesis in the equine fetal gonad"],"prefix":"10.1210","volume":"167","author":[{"given":"Katarzyna","family":"Malin","sequence":"first","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan J","family":"Conley","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margo","family":"Verstraete","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California , Davis, CA 95616 ,","place":["USA"]},{"name":"Department of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition, Faculty of Veterinary Medicine, Ghent University , 9820 Merelbeke ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Norris","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Chen","family":"Hsu","sequence":"additional","affiliation":[{"name":"West Coast Metabolomics Center, Genome Center, University of California , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Holl","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences and Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University , Baton Rouge, LA 70803 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Machteld","family":"van Heule","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California , Davis, CA 95616 ,","place":["USA"]},{"name":"Department of Morphology, Imaging, Orthopedics, Rehabilitation and Nutrition, Faculty of Veterinary Medicine, Ghent University , 9820 Merelbeke ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via","family":"Vieira","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten E","family":"Scoggin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky , Lexington, KY 40546 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry","family":"Ball","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky , Lexington, KY 40546 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuki","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"de la Fuente","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W","family":"Newman","sequence":"additional","affiliation":[{"name":"West Coast Metabolomics Center, Genome Center, University of California , Davis, CA 95616 ,","place":["USA"]},{"name":"Department of Nutrition, University of California Davis , Davis, CA 95616 ,","place":["USA"]},{"name":"Obesity and Metabolism Research Unit, USDA Western Human Research Center , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano","family":"Carossino","sequence":"additional","affiliation":[{"name":"Department of Pathobiological Sciences and Louisiana Animal Disease Diagnostic Laboratory, School of Veterinary Medicine, Louisiana State University , Baton Rouge, LA 70803 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5553-5967","authenticated-orcid":false,"given":"Pouya","family":"Dini","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction, School of Veterinary Medicine, University of California , Davis, CA 95616 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"issue":"3","key":"2026080301312724300_bqag083-B1","first-page":"449","article-title":"A brief account of the discovery of the fetal\/placental unit for 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Compartmentalization of DHCR7 links 7DHC metabolism to B-ring unsaturated steroid synthesis in the equine fetal gonad. Supplementary Figure 1. 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Compartmentalization of DHCR7 links 7DHC metabolism to B-ring unsaturated steroid synthesis in the equine fetal gonad. Supplementary Data 1. 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