{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:58:53Z","timestamp":1790445533544,"version":"4.1.0"},"reference-count":0,"publisher":"The Endocrine Society","license":[{"start":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:00:00Z","timestamp":1790380800000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Pancreatic \u03b2-cell dedifferentiation is a pathological mechanism that contributes to \u03b2-cell dysfunction and loss during type 2 diabetes (T2D), but the underlying mechanisms remain incompletely understood. MicroRNAs (miRNAs) are key post-transcriptional regulators of cellular identity and function. We previously identified miR-483 as a \u03b2-cell-enriched miRNA that promotes \u03b2-cell function by targeting Aldh1a3. Here, we investigated its role under high-fat diet (HFD)\/multiple low-dose streptozotocin (STZ)-induced metabolic stress. \u03b2-cell-specific miR-483 deficiency exacerbated hyperglycemia and impaired glucose tolerance, accompanied by increased ALDH1A3 expression and reduced insulin expression in GFP-labeled cells. Under HFD\/STZ-induced metabolic stress, miR-483 deficiency also increased glucagon production and the abundance of GFP+GCG+ and ALDH1A3+GCG+ cells, consistent with loss of \u03b2-cell identity and emergence of \u03b1-cell-like characteristics. Single-cell RNA sequencing revealed increased \u03b2-cells heterogeneity, including a subpopulation with glucagon expression and an altered endocrine phenotype. In addition, miR-483 deficiency was associated with mitochondrial fragmentation, impaired respiratory function, and increased oxidative stress. Together, these findings suggest that miR-483-ALDH1A3 axis contributes to the coordination of \u03b2-cell identity with mitochondrial and metabolic homeostasis during metabolic stress.<\/jats:p>","DOI":"10.1210\/endocr\/bqag110","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T11:17:40Z","timestamp":1790335060000},"source":"Crossref","is-referenced-by-count":0,"title":["miR-483 deficiency promotes loss of pancreatic beta-cell identity and aberrant glucagon production under metabolic stress"],"prefix":"10.1210","author":[{"given":"Katy","family":"Matson","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Michigan Technological University , Houghton, MI 49931"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"MacLeod","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Michigan Technological University , Houghton, MI 49931"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellie","family":"Sempek","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Michigan Technological University , Houghton, MI 49931"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan","family":"Noble","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Michigan Technological University , Houghton, MI 49931"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryce","family":"Tapert","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Michigan Technological University , Houghton, MI 49931"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhad","family":"Alipour","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Michigan Technological University , Houghton, MI 49931"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohu","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Michigan Technological University , Houghton, MI 49931"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aimee","family":"Marceau","sequence":"additional","affiliation":[{"name":"Genomic Sequencing Lab, Michigan Technological University , Houghton, MI 49931"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie S","family":"Satin","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Brehm Center for Diabetes, University of Michigan , Ann Arbor, MI 48109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3039-806X","authenticated-orcid":false,"given":"Xiaoqing","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Michigan Technological University , Houghton, MI 49931"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","published-online":{"date-parts":[[2026,9,26]]},"container-title":["Endocrinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/endo\/advance-article-pdf\/doi\/10.1210\/endocr\/bqag110\/71403454\/bqag110.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/endo\/advance-article-pdf\/doi\/10.1210\/endocr\/bqag110\/71403454\/bqag110.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:33:49Z","timestamp":1790444029000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/endo\/advance-article\/doi\/10.1210\/endocr\/bqag110\/8836685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,26]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1210\/endocr\/bqag110","relation":{},"ISSN":["1945-7170"],"issn-type":[{"value":"1945-7170","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,26]]},"article-number":"bqag110"}}