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To assess the function of ESR1 in reproductive tissues without confounding effects from a potential developmental defect arising from global deletion of ESR1, we generated a mouse model in which Esr1 was specifically ablated during postnatal development. To accomplish this, a progesterone receptor Cre line (PgrCre) was bred with Esr1f\/f mice to create conditional knockout of Esr1 in reproductive tissues (called PgrCreEsr1KO mice) beginning around 6 days after birth. In the PgrCreEsr1KO oviduct, ESR1 was most efficiently ablated in the isthmic region. We found that at 3.5 days post coitus (dpc), embryos were retrieved from the uterus in control littermates while all embryos were retained in the PgrCreEsr1KO oviduct. Additionally, serum progesterone (P4) levels were significantly lower in PgrCreEsr1KO compared to controls at 3.5 dpc. This finding suggests that expression of ESR1 in the isthmus and normal P4 levels allow for successful embryo transport from the oviduct to the uterus. Therefore, alterations in oviductal isthmus ESR1 signaling and circulating P4 levels could be related to female infertility conditions such as tubal pregnancy.<\/jats:p>","DOI":"10.1210\/endocr\/bqz033","type":"journal-article","created":{"date-parts":[[2019,12,28]],"date-time":"2019-12-28T05:07:46Z","timestamp":1577509666000},"source":"Crossref","is-referenced-by-count":18,"title":["Oviductal Retention of Embryos in Female Mice Lacking Estrogen Receptor \u03b1 in the Isthmus and the Uterus"],"prefix":"10.1210","volume":"161","author":[{"given":"Gerardo G B","family":"Herrera","sequence":"first","affiliation":[{"name":"School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, Washington, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sydney L","family":"Lierz","sequence":"first","affiliation":[{"name":"Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health (NIH\/NIEHS), Department of Health and Human Services, Research Triangle Park, North Carolina, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily A","family":"Harris","sequence":"first","affiliation":[{"name":"School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, Washington, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lauren J","family":"Donoghue","sequence":"first","affiliation":[{"name":"Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health (NIH\/NIEHS), Department of Health and Human Services, Research Triangle Park, North Carolina, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7713-0805","authenticated-orcid":false,"given":"Sylvia C","family":"Hewitt","sequence":"first","affiliation":[{"name":"Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health (NIH\/NIEHS), Department of Health and Human Services, Research Triangle Park, North Carolina, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina F","family":"Rodriguez","sequence":"first","affiliation":[{"name":"Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health (NIH\/NIEHS), Department of Health and Human Services, Research Triangle Park, North Carolina, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wendy N","family":"Jefferson","sequence":"first","affiliation":[{"name":"Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health (NIH\/NIEHS), Department of Health and Human Services, Research Triangle Park, North Carolina, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John P","family":"Lydon","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9480-7336","authenticated-orcid":false,"given":"Francesco J","family":"DeMayo","sequence":"first","affiliation":[{"name":"Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health (NIH\/NIEHS), Department of Health and Human Services, Research Triangle Park, North Carolina, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6440-7086","authenticated-orcid":false,"given":"Carmen J","family":"Williams","sequence":"first","affiliation":[{"name":"Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health (NIH\/NIEHS), Department of Health and Human Services, Research Triangle Park, North Carolina, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7765-418X","authenticated-orcid":false,"given":"Kenneth S","family":"Korach","sequence":"first","affiliation":[{"name":"Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health (NIH\/NIEHS), Department of Health and Human Services, Research Triangle Park, North Carolina, US"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5196-8471","authenticated-orcid":false,"given":"Wipawee","family":"Winuthayanon","sequence":"first","affiliation":[{"name":"School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, Washington, 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