{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T09:56:30Z","timestamp":1791107790222,"version":"4.1.0"},"reference-count":0,"publisher":"The Endocrine Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Aldosterone excess can produce heart and kidney fibrosis, which seem to be related to a direct effect of aldosterone at the level of specific receptors. We report a direct, mineralocorticoid-mediated effect on the protein expression of two markers of oxidative stress after incubation of mononuclear leukocytes with 1 \u00d7 10\u22128m aldosterone (p22phox\/\u03b2-actin = 1.38 \u00b1 0.05 and PAI-1\/\u03b2-actin = 1.80 \u00b1 0.05). The same effect was also found with 3 \u00d7 10\u22125m glycyrrhetinic acid, the principal constituent of licorice root (p22phox\/\u03b2-actin = 1.37 \u00b1 0.97 and PAI-1\/\u03b2-actin = 1.80 \u00b1 0.04). The effect of both aldosterone and glycyrrhetinic acid is blocked by incubation with added 1 \u00d7 10\u22126m of receptor-antagonist canrenone. Canrenone alone did not show any effect. PAI-1 related protein was also found using 4 \u00d7 10\u22129m aldosterone. Incubations with 1 \u00d7 10\u22129m for 3 hours as well as 1 \u00d7 10\u22128m aldosterone for 5, 10, and 20 minutes were ineffective for both proteins. These data support the previous finding of an involvement of mononuclear leukocytes in the pathogenesis of the oxidative stress induced by hyperaldosteronism. In addition, the results confirm our previous data on a direct effect of glycyrrhetinic acid at the level of mineralocorticoid receptors.<\/jats:p>","DOI":"10.1210\/jc.2003-031545","type":"journal-article","created":{"date-parts":[[2004,4,7]],"date-time":"2004-04-07T18:23:39Z","timestamp":1081362219000},"page":"1973-1976","source":"Crossref","is-referenced-by-count":115,"title":["Effect of Aldosterone and Glycyrrhetinic Acid on the Protein Expression of PAI-1 and p22phox in Human Mononuclear Leukocytes"],"prefix":"10.1210","volume":"89","author":[{"given":"Lorenzo A.","family":"Calo\u0300","sequence":"first","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Clinica Medica 4 (L.A.C., F.Z., E.P., P.D.M.), University of Padua, Padua 35100, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Zaghetto","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Clinica Medica 4 (L.A.C., F.Z., E.P., P.D.M.), University of Padua, Padua 35100, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Pagnin","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Clinica Medica 4 (L.A.C., F.Z., E.P., P.D.M.), University of Padua, Padua 35100, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A.","family":"Davis","sequence":"additional","affiliation":[{"name":"Departments of Nutrition, Epidemiology and Preventive Medicine (P.A.D.), University of California-Davis, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"de Mozzi","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Clinica Medica 4 (L.A.C., F.Z., E.P., P.D.M.), University of Padua, Padua 35100, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Sartorato","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Endocrinology (P.S., G.M., C.F., D.A.), University of Padua, Padua 35100, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Martire","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Endocrinology (P.S., G.M., C.F., D.A.), University of Padua, Padua 35100, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Fiore","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Endocrinology (P.S., G.M., C.F., D.A.), University of Padua, Padua 35100, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Decio","family":"Armanini","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Endocrinology (P.S., G.M., C.F., D.A.), University of Padua, Padua 35100, Italy;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","container-title":["The Journal of Clinical Endocrinology &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jcem\/article-pdf\/89\/4\/1973\/10744939\/jcem1973.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jcem\/article-pdf\/89\/4\/1973\/10744939\/jcem1973.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T23:57:24Z","timestamp":1594943844000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jcem\/article\/89\/4\/1973\/2844702"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,4,1]]}},"URL":"https:\/\/doi.org\/10.1210\/jc.2003-031545","relation":{},"ISSN":["0021-972X","1945-7197"],"issn-type":[{"value":"0021-972X","type":"print"},{"value":"1945-7197","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4,1]]}}}