{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:09:46Z","timestamp":1762916986308},"reference-count":38,"publisher":"The Endocrine Society","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,10,1]]},"abstract":"<jats:p>Context: \u03b23-Adrenoreceptor modulation in human myometrium during pregnancy is linked functionally to myometrial inhibition. Maxi-K+ channels (BKCa) play a significant role in modulating cell membrane potential and excitability.<\/jats:p>\n               <jats:p>Objective: This study was designed to investigate the potential involvement of BKCa channel function in the response of human myometrium to \u03b23-adrenoceptor activation.<\/jats:p>\n               <jats:p>Design: Single and whole-cell electrophysiological BKCa channel recordings from freshly dispersed myocytes were obtained in the presence and absence of BRL37344, a specific \u03b23-adrenoreceptor agonist. The in vitro effects of BRL37344 on isolated myometrial contractions, in the presence and absence of the specific BKCa channel blocker, iberiotoxin (IbTX), were investigated.<\/jats:p>\n               <jats:p>Setting: The study was carried out at the Clinical Science Institute.<\/jats:p>\n               <jats:p>Patients or Other Participants: Myometrial biopsies were obtained at elective cesarean delivery.<\/jats:p>\n               <jats:p>Intervention: No intervention was applied.<\/jats:p>\n               <jats:p>Main Outcome Measures: Open state probability of single channel recordings, whole cell currents, and myometrial contractile activity were measured.<\/jats:p>\n               <jats:p>Results: Single-channel recordings identified the BKCa channel as a target of BRL37344. BRL37344 significantly increased the open state probability of this channel in a concentration-dependent manner (control 0.031 \u00b1 0.004; 50 \u03bcm BRL37344 0.073 \u00b1 0.005 (P &amp;lt; 0.001); and 100 \u03bcm BRL37344 0.101 \u00b1 0.005 (P &amp;lt; 0.001). This effect was completely blocked after preincubation of the cells with 1 \u03bcm bupranolol, a nonspecific \u03b2-adrenoreceptor blocker, or 100 nm SR59230a, a specific \u03b23-adrenoreceptor antagonist. In addition, BRL37344 increased whole-cell currents over a range of membrane potentials, and this effect was reversed by 100 nm IbTX. In vitro isometric tension studies demonstrated that BRL37344 exerted a significant concentration-dependent relaxant effect on human myometrial tissue (P &amp;lt; 0.05), and preincubation of these strips with IbTX attenuated this effect on both spontaneous and oxytocin-induced contractions (44.44 and 57.84% at 10\u22125m, respectively).<\/jats:p>\n               <jats:p>Conclusions: These findings outline that activation of the BKCa channel may explain the potent uterorelaxant effect of \u03b23-adrenoreceptor agonists.<\/jats:p>","DOI":"10.1210\/jc.2005-0574","type":"journal-article","created":{"date-parts":[[2005,10,5]],"date-time":"2005-10-05T22:02:56Z","timestamp":1128549776000},"page":"5786-5796","source":"Crossref","is-referenced-by-count":30,"title":["Functional Coupling of \u03b23-Adrenoceptors and Large Conductance Calcium-Activated Potassium Channels in Human Uterine Myocytes"],"prefix":"10.1210","volume":"90","author":[{"given":"Helen C.","family":"Doheny","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology (H.C.D., C.M.L., J.J.M.), National University of Ireland Galway, Clinical Science Institute, University College Hospital Galway, Galway, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caoimhe M.","family":"Lynch","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology (H.C.D., C.M.L., J.J.M.), National University of Ireland Galway, Clinical Science Institute, University College Hospital Galway, Galway, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry J.","family":"Smith","sequence":"additional","affiliation":[{"name":"National Centre for Biomedical Engineering Science (T.J.S., J.J.M.), National University of Ireland, Galway, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Morrison","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology (H.C.D., C.M.L., J.J.M.), National University of Ireland Galway, Clinical Science Institute, University College Hospital Galway, Galway, Ireland"},{"name":"National Centre for Biomedical Engineering Science (T.J.S., J.J.M.), National University of Ireland, Galway, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"key":"2020071617474345600_R1","doi-asserted-by":"crossref","first-page":"1648","DOI":"10.1067\/mob.2003.356","article-title":"Tocolytic treatment for the management of preterm labor: a review of the evidence.","volume":"188","author":"Berkman","year":"2003","journal-title":"Am J Obstet Gynecol"},{"key":"2020071617474345600_R2","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1016\/S0140-6736(02)11476-0","article-title":"Preterm 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