{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:06:33Z","timestamp":1783487193898,"version":"3.55.0"},"reference-count":40,"publisher":"The Endocrine Society","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Context: Thyroglobulin (Tg) epitopes of serum Tg autoantibodies (TgAb) have been characterized using inhibition of Tg binding by human monoclonal TgAb in autoimmune thyroid diseases (AITD) [Hashimoto\u2019s thyroiditis (HT) and Graves\u2019 disease (GD)] but not in non-AITD [nontoxic multinodular goiter (NTMG) and papillary thyroid carcinoma (PTC)].<\/jats:p><jats:p>Objective: Our objective was to compare Tg epitopes of serum TgAb from patients with AITD, non-AITD, and PTC associated with histological thyroiditis (PTC-T) using inhibition of Tg binding by four recombinant human TgAb-Fab (epitopic regions A\u2013D).<\/jats:p><jats:p>Design: Inhibition of Tg binding of 24 HT, 25 GD, 19 NTMG, 15 PTC, and 25 PTC-T TgAb-positive sera by each TgAb-Fab was evaluated in ELISA. Inhibition by the pool of the four TgAb-Fab was evaluated using labeled Tg.<\/jats:p><jats:p>Results: Levels of inhibition were different for TgAb-Fab regions A (P = 0.001), B (0.007), and D (0.011). Inhibition by region A TgAb-Fab was significantly higher in HT, GD, and PTC-T than in NTMG and PTC patients. Inhibition levels by region B TgAb-Fab were significantly higher in HT compared with NTMG and PTC patients and in GD compared with NTMG patients. Inhibition by D region TgAb-Fab was significantly lower in NTMG than in the other groups. Inhibition by the pool ranged from 44% (NTMG) to 72% (GD).<\/jats:p><jats:p>Conclusions: The pattern of Tg recognition is similar when HT patients are compared to GD and NTMG to PTC patients and differs when AITD are compared with non-AITD patients. In PTC-T patients, it is similar to that of AITD patients.<\/jats:p>","DOI":"10.1210\/jc.2007-1199","type":"journal-article","created":{"date-parts":[[2007,11,21]],"date-time":"2007-11-21T01:48:35Z","timestamp":1195609715000},"page":"591-596","source":"Crossref","is-referenced-by-count":72,"title":["Characterization of Thyroglobulin Epitopes in Patients with Autoimmune and Non-Autoimmune Thyroid Diseases Using Recombinant Human Monoclonal Thyroglobulin Autoantibodies"],"prefix":"10.1210","volume":"93","author":[{"given":"Francesco","family":"Latrofa","sequence":"first","affiliation":[{"name":"Department of Endocrinology (F.L., D.R., L.G., P.V., A.P.), World Health Organization Collaborating Center for the Diagnosis and Treatment of Thyroid Cancer and Other Thyroid Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debora","family":"Ricci","sequence":"additional","affiliation":[{"name":"Department of Endocrinology (F.L., D.R., L.G., P.V., A.P.), World Health Organization Collaborating Center for the Diagnosis and Treatment of Thyroid Cancer and Other Thyroid Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Grasso","sequence":"additional","affiliation":[{"name":"Department of Endocrinology (F.L., D.R., L.G., P.V., A.P.), World Health Organization Collaborating Center for the Diagnosis and Treatment of Thyroid Cancer and Other Thyroid Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Vitti","sequence":"additional","affiliation":[{"name":"Department of Endocrinology (F.L., D.R., L.G., P.V., A.P.), World Health Organization Collaborating Center for the Diagnosis and Treatment of Thyroid Cancer and Other Thyroid Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucio","family":"Masserini","sequence":"additional","affiliation":[{"name":"Department of Statistic and Economy (L.M.), Section of Pathology 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fulvio","family":"Basolo","sequence":"additional","affiliation":[{"name":"Department of Oncology (F.B., C.U.), Section of Pathology 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Ugolini","sequence":"additional","affiliation":[{"name":"Department of Oncology (F.B., C.U.), Section of Pathology 3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Mascia","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Neurobiology, Pharmacology, and Biotechnology (G.M., A.L.), University of Pisa, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Lucacchini","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, Neurobiology, Pharmacology, and Biotechnology (G.M., A.L.), University of Pisa, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldo","family":"Pinchera","sequence":"additional","affiliation":[{"name":"Department of Endocrinology (F.L., D.R., L.G., P.V., A.P.), World Health Organization Collaborating Center for the Diagnosis and Treatment of Thyroid Cancer and Other Thyroid Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071613441190400_R1","first-page":"136","article-title":"Autoimmune hypothyroidism","author":"Latrofa","year":"2007","journal-title":"In: Weetman AP, ed. 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