{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T14:40:38Z","timestamp":1783348838838,"version":"3.54.6"},"reference-count":40,"publisher":"The Endocrine Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Context: Lysophosphatidic acid (LPA) was found at significant amounts in follicular fluid of preovulatory follicle. The lysophospholipase D activity of serum from women receiving ovarian stimulation was higher than women with natural cycles. Angiogenic cytokines, including IL-6, IL-8, and vascular endothelial growth factor, increased in plasma and ascites of patients with ovarian hyperstimulation syndrome. The role of LPA in ovarian follicles is unclear.<\/jats:p>\n               <jats:p>Objective: Our objective was to investigate the expression of LPA receptors and function of LPA in granulosa-lutein cells.<\/jats:p>\n               <jats:p>Design: Granulosa-lutein cells were obtained from women undergoing in vitro fertilization. We examined the expression of LPA receptors using RT-PCR. The effects of LPA on the expression of IL-6, IL-8, and vascular endothelial growth factor were examined. Signal pathways of LPA were delineated. The functions of secretory angiogenic factors were tested using human umbilical vein endothelial cells.<\/jats:p>\n               <jats:p>Results: The LPA1, LPA2, and LPA3 receptors\u2019 mRNA was identified in granulosa-lutein cells. LPA enhanced IL-8 and IL-6 expressions in a dose- and time-dependent manner. LPA functioned via LPA receptors, Gi protein, MAPK\/ERK, p38, phosphatidylinositol 3-kinase\/Akt, and nuclear factor-\u03baB, and transactivation of epidermal growth factor receptor. LPA induced IL-8 and IL-6 through different pathways. LPA-induced IL-8 and IL-6 increased permeability of human umbilical vein endothelial cell monolayer.<\/jats:p>\n               <jats:p>Conclusions: LPA induces IL-8 and IL-6 expressions through LPA receptors and nuclear factor-\u03baB dependent pathways in granulosa-lutein cells. The LPA in preovulatory follicles may play a role in the angiogenesis of corpus luteum. Large amounts of LPA-induced IL-8 and IL-6 from multiple corpora luteae of stimulated ovaries may be one of the pathophysiological causes of ovarian hyperstimulation syndrome.<\/jats:p>","DOI":"10.1210\/jc.2007-1512","type":"journal-article","created":{"date-parts":[[2008,1,3]],"date-time":"2008-01-03T01:56:33Z","timestamp":1199325393000},"page":"935-943","source":"Crossref","is-referenced-by-count":62,"title":["Lysophosphatidic Acid Up-Regulates Expression of Interleukin-8 and -6 in Granulosa-Lutein Cells through Its Receptors and Nuclear Factor-\u03baB Dependent Pathways: Implications for Angiogenesis of Corpus Luteum and Ovarian Hyperstimulation Syndrome"],"prefix":"10.1210","volume":"93","author":[{"given":"Shee-Uan","family":"Chen","sequence":"first","affiliation":[{"name":"Departments of Obstetrics and Gynecology (S.-U.C., C.-H.C., C.-H.H., Y.-S.Y.), National Taiwan University, Taipei, 100 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia-Hung","family":"Chou","sequence":"additional","affiliation":[{"name":"Departments of Obstetrics and Gynecology (S.-U.C., C.-H.C., C.-H.H., Y.-S.Y.), National Taiwan University, Taipei, 100 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsinyu","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Science (H.L.), National Taiwan University, Taipei, 100 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Hong","family":"Ho","sequence":"additional","affiliation":[{"name":"Departments of Obstetrics and Gynecology (S.-U.C., C.-H.C., C.-H.H., Y.-S.Y.), National Taiwan University, Taipei, 100 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung-Wu","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pathology (C.-W.L.), National Taiwan University, Taipei, 100 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