{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T12:55:26Z","timestamp":1771332926905,"version":"3.50.1"},"reference-count":20,"publisher":"The Endocrine Society","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Paget\u2019s disease is a condition of focal accelerated bone turnover. Electron-microscopy investigations of osteoclasts from pagetic lesions have identified nuclear inclusion bodies that have a similar appearance to viral nucleocapsid particles. Subsequently, RNA from several paramyxoviruses has been detected in pagetic tissue, and it was suggested that these viruses, in particular measles, might play a role in the etiology of Paget\u2019s disease. We have tested for measles virus sequences in osteoblasts and bone marrow cells collected from pagetic lesions and healthy bone.<\/jats:p>\n               <jats:p>Methods: Bone and bone marrow samples were taken from Paget\u2019s patients and control subjects, and cells were cultured from each of these tissues. RNA was extracted from 13 osteoblast cultures and 13 cultures of bone marrow cells derived from pagetic lesions, and from 26 and 23 control osteoblast and bone marrow cultures, respectively. These samples were sourced from 22 patients with Paget\u2019s disease and 31 controls. RT-PCR-nested PCR amplification was used for the detection of the genes for the measles nucleocapsid and matrix proteins.<\/jats:p>\n               <jats:p>Results: Measles virus sequences were not detected in any of the pagetic or control samples. However, measles virus sequences were identified in samples of a measles virus culture isolate included as a positive control, and in a brain sample from a patient with subacute sclerosing panencephalitis, a condition associated with chronic measles infection.<\/jats:p>\n               <jats:p>Conclusion: The results of the study do not support the hypothesis that measles virus plays a role in the pathogenesis of Paget\u2019s disease.<\/jats:p>","DOI":"10.1210\/jc.2007-1978","type":"journal-article","created":{"date-parts":[[2008,1,30]],"date-time":"2008-01-30T04:50:39Z","timestamp":1201668639000},"page":"1398-1401","source":"Crossref","is-referenced-by-count":56,"title":["Failure to Detect Measles Virus Ribonucleic Acid in Bone Cells from Patients with Paget\u2019s Disease"],"prefix":"10.1210","volume":"93","author":[{"given":"Brya G.","family":"Matthews","sequence":"first","affiliation":[{"name":"Departments of Medicine (B.G.M., U.B., K.E.C., T.C., J.C., I.R.R., D.N.), University of Auckland, Auckland 1142, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad A.","family":"Afzal","sequence":"additional","affiliation":[{"name":"National Institute for Biological Standards and Control (M.A.A., P.D.M.), Hertfordshire EN6 3QG, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip D.","family":"Minor","sequence":"additional","affiliation":[{"name":"National Institute for Biological Standards and Control (M.A.A., P.D.M.), Hertfordshire EN6 3QG, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Usha","family":"Bava","sequence":"additional","affiliation":[{"name":"Departments of Medicine (B.G.M., U.B., K.E.C., T.C., J.C., I.R.R., D.N.), University of Auckland, Auckland 1142, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen E.","family":"Callon","sequence":"additional","affiliation":[{"name":"Departments of Medicine (B.G.M., U.B., K.E.C., T.C., J.C., I.R.R., D.N.), University of Auckland, Auckland 1142, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rocco P.","family":"Pitto","sequence":"additional","affiliation":[{"name":"Surgery (R.P.P.), University of Auckland, Auckland 1142, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Cundy","sequence":"additional","affiliation":[{"name":"Departments of Medicine (B.G.M., U.B., K.E.C., T.C., J.C., I.R.R., D.N.), University of Auckland, Auckland 1142, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jill","family":"Cornish","sequence":"additional","affiliation":[{"name":"Departments of Medicine (B.G.M., U.B., K.E.C., T.C., J.C., I.R.R., D.N.), University of Auckland, Auckland 1142, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian R.","family":"Reid","sequence":"additional","affiliation":[{"name":"Departments of Medicine (B.G.M., U.B., K.E.C., T.C., J.C., I.R.R., D.N.), University of Auckland, Auckland 1142, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorit","family":"Naot","sequence":"additional","affiliation":[{"name":"Departments of Medicine (B.G.M., U.B., K.E.C., T.C., J.C., I.R.R., D.N.), University of Auckland, Auckland 1142, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"issue":"Pt 10","key":"2020071615461065600_R1","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1099\/0022-1317-66-10-2103","article-title":"Paramyxovirus antigens in osteoclasts from Paget\u2019s bone tissue detected by monoclonal antibodies","volume":"66","author":"Basle","year":"1985","journal-title":"J Gen Virol"},{"issue":"Pt 5","key":"2020071615461065600_R2","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1099\/0022-1317-67-5-907","article-title":"Measles virus RNA detected in Paget\u2019s disease bone tissue by in situ hybridization","volume":"67","author":"Basle","year":"1986","journal-title":"J Gen Virol"},{"key":"2020071615461065600_R3","first-page":"2108","article-title":"Bone marrow mononuclear cells from patients with Paget\u2019s disease contain measles virus nucleocapsid messenger ribonucleic acid that has mutations in a specific region of the sequence.","volume":"80","author":"Reddy","year":"1995","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071615461065600_R4","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1016\/S0301-472X(99)00097-1","article-title":"Measles virus nucleocapsid transcript expression is not restricted to the osteoclast lineage in patients with Paget\u2019s disease of bone.","volume":"27","author":"Reddy","year":"1999","journal-title":"Exp Hematol"},{"key":"2020071615461065600_R5","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1359\/jbmr.2002.17.1.145","article-title":"Sequence analysis of measles virus nucleocapsid transcripts in patients with Paget\u2019s disease.","volume":"17","author":"Friedrichs","year":"2002","journal-title":"J Bone Miner Res"},{"key":"2020071615461065600_R6","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/jbmr.5650090103","article-title":"Absence of paramyxovirus RNA in cultures of pagetic bone-cells and in pagetic bone.","volume":"9","author":"Birch","year":"1994","journal-title":"J Bone Miner Res"},{"key":"2020071615461065600_R7","doi-asserted-by":"crossref","first-page":"2315","DOI":"10.1359\/jbmr.2000.15.12.2315","article-title":"A negative search for a paramyxoviral etiology of Paget\u2019s disease of bone: molecular, immunological, and ultrastructural studies in UK patients.","volume":"15","author":"Helfrich","year":"2000","journal-title":"J Bone Miner Res"},{"key":"2020071615461065600_R8","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/S8756-3282(00)00343-4","article-title":"Absence of measles virus and canine distemper virus transcripts in long-term bone 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