{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T22:48:28Z","timestamp":1784674108808,"version":"3.55.0"},"reference-count":44,"publisher":"The Endocrine Society","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,1]]},"abstract":"<jats:sec>\n                  <jats:title>Context:<\/jats:title>\n                  <jats:p>Aldosterone (Aldo) effects include NADPH oxidase activation involved in Aldo-related oxidative stress. Red blood cells (RBCs) are particularly sensitive to oxidative assault, and both the formation of high molecular weight aggregates (HMWAs) and the diamide-induced Tyr phosphorylation (Tyr-P) level of membrane band 3 can be used to monitor their redox status.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objective:<\/jats:title>\n                  <jats:p>The Aldo-related alterations in erythrocytes were evaluated by comparing in vitro evidence.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Design:<\/jats:title>\n                  <jats:p>This was a multicenter comparative study.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Study Participants:<\/jats:title>\n                  <jats:p>The study included 12 patients affected by primary aldosteronism (PA) and 6 healthy control subjects (HCs), whose RBCs were compared with those of patients with PA. For in vitro experiments, RBCs from HCs were incubated with increasing Aldo concentrations.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Main Outcome Measures:<\/jats:title>\n                  <jats:p>The Tyr-P level, band 3 HMWA formation, and autologous IgG binding were evaluated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results:<\/jats:title>\n                  <jats:p>In patients with PA, both Tyr-P levels and band 3 HMWAs were higher than those in HCs. RBCs from HCs were treated with increasing Aldo concentrations in both platelet-poor plasma (PPP) and charcoal-stripped (CS)-PPP. Results showed that Aldo had dose- and time-dependent effects on band 3 Tyr-P and HMWA formation in CS-PPP more than in PPP. These effects were almost completely prevented by canrenone or cortisol. Aldo-related membrane alterations led to increased autologous IgG binding.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions:<\/jats:title>\n                  <jats:p>Erythrocytes from patients with PA show oxidative-like stress evidenced by increased HMWA content and diamide-induced band 3 Tyr-P level. Aldo effects are mediated by the mineralocorticoid receptor, as suggested by the inhibitory effects of canrenone, an antagonist of Aldo. In CS-PPP, in which Aldo induces remarkable membrane alterations leading to IgG binding, Aldo may be responsible for premature RBC removal from circulation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1210\/jc.2012-3571","type":"journal-article","created":{"date-parts":[[2013,3,29]],"date-time":"2013-03-29T05:43:12Z","timestamp":1364535792000},"page":"2494-2501","source":"Crossref","is-referenced-by-count":22,"title":["Human Red Blood Cells Alterations in Primary Aldosteronism"],"prefix":"10.1210","volume":"98","author":[{"given":"Luciana","family":"Bordin","sequence":"first","affiliation":[{"name":"Department of Molecular Medicine-Biological Chemistry (L.B., G.D., A.M.B., G.C.), 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriella","family":"Don\u00e0","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine-Biological Chemistry (L.B., G.D., A.M.B., G.C.), 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Sabbadin","sequence":"additional","affiliation":[{"name":"Department of Medicine-Endocrinology (C.S., D.A.), 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenio","family":"Ragazzi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences (E.R.), 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Andrisani","sequence":"additional","affiliation":[{"name":"Department of Women's Health-Salus Pueri (G.A., A.A.), University of Padua, 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Ambrosini","sequence":"additional","affiliation":[{"name":"Department of Women's Health-Salus Pueri (G.A., A.A.), University of Padua, 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Maria","family":"Brunati","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine-Biological Chemistry (L.B., G.D., A.M.B., G.C.), 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Clari","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine-Biological Chemistry (L.B., G.D., A.M.B., G.C.), 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Decio","family":"Armanini","sequence":"additional","affiliation":[{"name":"Department of Medicine-Endocrinology (C.S., D.A.), 35131 Padua, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"80","reference":[{"key":"2020071613240297100_B1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1152\/advan.00051.2001","article-title":"Aldosterone","volume":"26","author":"Booth","year":"2002","journal-title":"Adv Physiol Educ"},{"key":"2020071613240297100_B2","doi-asserted-by":"crossref","first-page":"2712","DOI":"10.1073\/pnas.051603198","article-title":"Transcriptome of a mouse kidney cortical collecting duct cell line: effects of aldosterone and vasopressin","volume":"98","author":"Robert-Nicoud","year":"2001","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071613240297100_B3","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.mce.2011.07.001","article-title":"Interactions of the mineralocorticoid receptor\u2014within and without","volume":"350","author":"Yang","year":"2012","journal-title":"Mol Cell Endocrinol"},{"key":"2020071613240297100_B4","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1210\/me.2010-0193","article-title":"Identification of ligand-selective peptide antagonists of the mineralocorticoid receptor using phage display","volume":"25","author":"Yang","year":"2011","journal-title":"Mol Endocrinol"},{"key":"2020071613240297100_B5","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1093\/ndt\/gfg265","article-title":"Non-genomic actions of aldosterone: mechanisms and consequences in kidney cells","volume":"18","author":"Boldyreff","year":"2003","journal-title":"Nephrol Dial Transplant"},{"key":"2020071613240297100_B6","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.mce.2011.07.019","article-title":"Non-genomic actions of aldosterone: from receptors and signals to membrane targets","volume":"350","author":"Dooley","year":"2012","journal-title":"Mol Cell Endocrinol"},{"key":"2020071613240297100_B7","doi-asserted-by":"crossref","first-page":"2098","DOI":"10.1093\/eurheartj\/ehr266","article-title":"Aldosterone promotes atrial fibrillation","volume":"33","author":"Reil","year":"2012","journal-title":"Eur Heart J"},{"key":"2020071613240297100_B8","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1126\/science.1202887","article-title":"Medicine. 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