{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T08:20:09Z","timestamp":1779178809525,"version":"3.51.4"},"reference-count":38,"publisher":"The Endocrine Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,3,1]]},"abstract":"<jats:sec>\n                  <jats:title>Context:<\/jats:title>\n                  <jats:p>Inflammation in the pancreas can cause \u03b2-cell stress, leading to diabetes development. Access to human pancreas tissues via the Network for Pancreatic Organ Donors with Diabetes (nPOD) has allowed characterization of pathways leading to this inflammation.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objective:<\/jats:title>\n                  <jats:p>12-Lipoxygenase (12-LO) induces inflammation and has been implicated in diabetes development. Our goal was to determine expression of 12-LO in human islets from control, autoantibody-positive, type 1 diabetic, and type 2 diabetic nPOD pancreas donors.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Design:<\/jats:title>\n                  <jats:p>Pancreas tissues from nPOD donors were examined by immunohistochemistry and immunofluorescence for islet expression of 12-LO in different subsets of islet cells.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Participants:<\/jats:title>\n                  <jats:p>Donor pancreas samples were obtained from nPOD based on disease status (control, n = 7; autoantibody-positive, n = 8; type 1 diabetic, n = 17; or type 2 diabetic donors, n = 15).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Main Outcome Measure:<\/jats:title>\n                  <jats:p>Determination of 12-LO expression within human islets served as the main outcome measure, including distinguishing which types of islet cells expressed 12-LO.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results:<\/jats:title>\n                  <jats:p>Islets from control participants (nondiabetic) lacked islet expression of 12-LO. Of donors in the other groups, 25% to 37% expressed islet 12-LO with a clear inverse relation between the numbers of \u03b2-cells and 12-LO+ cells within islets of 12-LO+ cases. 12-LO expression was not seen within macrophages, endothelial cells, \u03b1-cells, or \u03b2-cells, but only within cells expressing low levels of pancreatic polypeptide (PP) and increased levels of vimentin.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions:<\/jats:title>\n                  <jats:p>12-LO expression colocalizes within a specific type of islet PP+ cell under prediabetic and diabetic conditions. The costaining of PP and vimentin suggests that 12-LO participates in the process leading to \u03b2-cell dedifferentiation in the islet.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1210\/jc.2014-3630","type":"journal-article","created":{"date-parts":[[2014,12,22]],"date-time":"2014-12-22T17:46:46Z","timestamp":1419270406000},"page":"E387-E395","source":"Crossref","is-referenced-by-count":37,"title":["Expression Pattern of 12-Lipoxygenase in Human Islets With Type 1 Diabetes and Type 2 Diabetes"],"prefix":"10.1210","volume":"100","author":[{"given":"Wojciech J.","family":"Grzesik","sequence":"first","affiliation":[{"name":"Department of Internal Medicine (W.J.G., J.L.N., Y.M., J.L.N., Y.I.), Norfolk, Virginia 23507"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph L.","family":"Nadler","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine (W.J.G., J.L.N., Y.M., J.L.N., Y.I.), Norfolk, Virginia 23507"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yui","family":"Machida","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine (W.J.G., J.L.N., Y.M., J.L.N., Y.I.), Norfolk, Virginia 23507"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry L.","family":"Nadler","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine (W.J.G., J.L.N., Y.M., J.L.N., Y.I.), Norfolk, Virginia 23507"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumi","family":"Imai","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine (W.J.G., J.L.N., Y.M., J.L.N., Y.I.), Norfolk, Virginia 23507"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret A.","family":"Morris","sequence":"additional","affiliation":[{"name":"Strelitz Diabetes Center, and Department of Microbiology and Molecular Cell Biology (M.A.M.), Eastern Virginia Medical School, Norfolk, Virginia 23507"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","reference":[{"key":"2020071615585066500_B1","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.plipres.2010.10.005","article-title":"Functional and pathological roles of the 12- and 15-lipoxygenases","volume":"50","author":"Dobrian","year":"2011","journal-title":"Prog Lipid Res"},{"key":"2020071615585066500_B2","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1016\/j.plipres.2006.02.003","article-title":"Inflammation and immune regulation by 12\/15-lipoxygenases","volume":"45","author":"Kuhn","year":"2006","journal-title":"Prog Lipids Res"},{"key":"2020071615585066500_B3","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1126\/science.294.5548.1871","article-title":"Prostaglandins and leukotrienes: advances in eicosanoid biology","volume":"294","author":"Funk","year":"2001","journal-title":"Science"},{"key":"2020071615585066500_B4","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.mce.2012.03.004","article-title":"Integration of pro-inflammatory cytokine, 12-lipoxygenase and NOX-1 in pancreatic islet \u03b2 cell dysfunction","volume":"358","author":"Weaver","year":"2012","journal-title":"Mol Cell Endocrinol"},{"key":"2020071615585066500_B5","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1210\/jc.2009-1102","article-title":"12-Lipoxygenase products reduce insulin secretion and \u03b2-cell viability in human islets","volume":"95","author":"Ma","year":"2010","journal-title":"J. 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