{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T03:32:17Z","timestamp":1768707137497,"version":"3.49.0"},"reference-count":48,"publisher":"The Endocrine Society","issue":"1","funder":[{"DOI":"10.13039\/100008269","name":"Michigan Institute for Clinical and Health Research","doi-asserted-by":"publisher","award":["UL1TR000433"],"award-info":[{"award-number":["UL1TR000433"]}],"id":[{"id":"10.13039\/100008269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008269","name":"Michigan Institute for Clinical and Health Research","doi-asserted-by":"publisher","award":["R01DK069950"],"award-info":[{"award-number":["R01DK069950"]}],"id":[{"id":"10.13039\/100008269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008269","name":"Michigan Institute for Clinical and Health Research","doi-asserted-by":"publisher","award":["R01DK43140"],"award-info":[{"award-number":["R01DK43140"]}],"id":[{"id":"10.13039\/100008269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008269","name":"Michigan Institute for Clinical and Health Research","doi-asserted-by":"publisher","award":["R01GM086596"],"award-info":[{"award-number":["R01GM086596"]}],"id":[{"id":"10.13039\/100008269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Dehydroepiandrosterone sulfate (DHEAS) is the most abundant steroid in human circulation, and adrenocorticotropic hormone (ACTH) is considered the major regulator of its synthesis. Pregnenolone sulfate (PregS) and 5-androstenediol-3-sulfate (AdiolS) have recently emerged as biomarkers of adrenal disorders.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To define the relative human adrenal production of \u03945-steroid sulfates under basal and cosyntropin-stimulated conditions.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Liquid chromatography-tandem mass spectrometry was used to quantify three unconjugated and four sulfated \u03945-steroids in (1) paired adrenal vein (AV) and mixed venous serum samples (21 patients) and (2) cultured human adrenal cells both before and after cosyntropin stimulation, (3) microdissected zona fasciculata (ZF) and zona reticularis (ZR) from five human adrenal glands, and (4) a reconstituted in vitro human 17\u03b1-hydroxylase\/17,20-lyase\/(P450 17A1) system.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Of the steroid sulfates, PregS had the greatest increase after cosyntropin stimulation in the AV (32-fold), whereas DHEAS responded modestly (1.8-fold). PregS attained concentrations comparable to those of DHEAS in the AV after cosyntropin stimulation (AV DHEAS\/PregS, 24 and 1.3 before and after cosyntropin, respectively). In cultured adrenal cells, PregS demonstrated the sharpest response to cosyntropin, whereas DHEAS responded only modestly (21-fold vs 1.8-fold higher compared with unstimulated cells at 3 hours, respectively). Steroid analyses in isolated ZF and ZR showed similar amounts of PregS and 17\u03b1-hydroxypregnenolone in both zones, whereas DHEAS and AdiolS were higher in ZR (P &amp;lt; 0.05).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Our studies demonstrated that unlike DHEAS, PregS displayed a prominent acute response to cosyntropin. PregS could be used to interrogate the acute adrenal response to ACTH stimulation and as a biomarker in various adrenal disorders.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1210\/jc.2017-01525","type":"journal-article","created":{"date-parts":[[2017,11,6]],"date-time":"2017-11-06T19:07:20Z","timestamp":1509995240000},"page":"320-327","source":"Crossref","is-referenced-by-count":29,"title":["Adrenocorticotropin Acutely Regulates Pregnenolone Sulfate Production by the Human Adrenal In Vivo and In Vitro"],"prefix":"10.1210","volume":"103","author":[{"given":"Juilee","family":"Rege","sequence":"first","affiliation":[{"name":"Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aya T","family":"Nanba","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Diabetes, University of Michigan, Ann Arbor, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard J","family":"Auchus","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Diabetes, University of Michigan, Ann Arbor, Michigan"},{"name":"Department of Pharmacology, University of Michigan, Ann Arbor, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianwei","family":"Ren","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Diabetes, University of Michigan, Ann Arbor, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hwei-Ming","family":"Peng","sequence":"additional","affiliation":[{"name":"Division of Metabolism, Endocrinology, and Diabetes, University of Michigan, Ann Arbor, Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William E","family":"Rainey","sequence":"additional","affiliation":[{"name":"Department of Molecular and Integrative Physiology, 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