{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T19:13:03Z","timestamp":1762542783114},"reference-count":41,"publisher":"The Endocrine Society","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,3,1]]},"abstract":"Abstract<\/jats:title>Context<\/jats:title>During normal, early puberty, luteinizing hormone (LH) pulse frequency is low while awake but increases during sleep. Mechanisms underlying such changes are unclear, but a small study in early pubertal girls suggested that differential wake-sleep sensitivity to progesterone negative feedback plays a role.<\/jats:p><\/jats:sec>Objective<\/jats:title>To test the hypothesis that progesterone acutely reduces waking LH pulse frequency more than sleep-associated pulse frequency in late pubertal girls.<\/jats:p><\/jats:sec>Design<\/jats:title>Randomized, placebo-controlled, double-blinded crossover study.<\/jats:p><\/jats:sec>Setting<\/jats:title>Academic clinical research unit.<\/jats:p><\/jats:sec>Participants<\/jats:title>Eleven normal, postmenarcheal girls, ages 12 to 15 years.<\/jats:p><\/jats:sec>Intervention<\/jats:title>Subjects completed two 18-hour admissions in separate menstrual cycles (cycle days 6 to 11). Frequent blood sampling for LH assessment was performed at 1800 to 1200 hours; sleep was encouraged at 2300 to 0700 hours. Either oral micronized progesterone (0.8 mg\/kg\/dose) or placebo was given at 0700, 1500, 2300, and 0700 hours, before and during the first admission. A second admission, performed at least 2 months later, was identical to the first except that placebo was exchanged for progesterone or vice versa (treatment crossover).<\/jats:p><\/jats:sec>Main Outcome Measures<\/jats:title>LH pulse frequency during waking and sleeping hours.<\/jats:p><\/jats:sec>Results<\/jats:title>Progesterone reduced waking LH pulse frequency by 26% (P = 0.019), with no change observed during sleep (P = 0.314). The interaction between treatment condition (progesterone vs placebo) and sleep status (wake vs sleep) was highly significant (P = 0.007).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>In late pubertal girls, progesterone acutely reduced waking LH pulse frequency more than sleep-associated pulse frequency. Differential wake-sleep sensitivity to progesterone negative feedback may direct sleep-wake LH pulse frequency changes across puberty.<\/jats:p><\/jats:sec>","DOI":"10.1210\/jc.2017-02299","type":"journal-article","created":{"date-parts":[[2017,12,28]],"date-time":"2017-12-28T19:20:44Z","timestamp":1514488844000},"page":"1112-1121","source":"Crossref","is-referenced-by-count":11,"title":["Progesterone-Mediated Inhibition of the GnRH Pulse Generator: Differential Sensitivity as a Function of Sleep Status"],"prefix":"10.1210","volume":"103","author":[{"given":"Su Hee","family":"Kim","sequence":"first","affiliation":[{"name":"Center for Research in Reproduction, University of Virginia School of Medicine, Charlottesville, Virginia"},{"name":"Division of Endocrinology, Department of Medicine, University of Virginia School of Medicine, Charlottesville, Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica 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