{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T15:38:09Z","timestamp":1776872289823,"version":"3.51.2"},"reference-count":0,"publisher":"The Endocrine Society","funder":[{"DOI":"10.13039\/100000071","name":"National Institute of Child Health and Human Development","doi-asserted-by":"publisher","award":["P01HD057877"],"award-info":[{"award-number":["P01HD057877"]}],"id":[{"id":"10.13039\/100000071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"Abstract<\/jats:title>\n \n Context<\/jats:title>\n Uterine leiomyoma (fibroids) are the most common tumors in women. Recently, perilipin-2 (PLIN2) was identified as a critical target gene of the progesterone receptor; however, its function in the pathogenesis of fibroids is unknown.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n To determine the function of PLIN2 in leiomyoma cells<\/jats:p>\n <\/jats:sec>\n \n Design<\/jats:title>\n Tissue and primary cells from leiomyoma and myometrium were analyzed. PLIN2 function in leiomyoma was assessed using siRNA. RNA-sequencing was performed to identify genome-wide effects of PLIN2 depletion. Metabolic activity was measured using the Seahorse XF96 Analyzer. Real-time qPCR and immunoblotting were also performed.<\/jats:p>\n <\/jats:sec>\n \n Setting<\/jats:title>\n Laboratory<\/jats:p>\n <\/jats:sec>\n \n Patients or Other Participants<\/jats:title>\n . 41 premenopausal women undergoing surgery for fibroids<\/jats:p>\n <\/jats:sec>\n \n Intervention(s)<\/jats:title>\n None<\/jats:p>\n <\/jats:sec>\n \n Main Outcome Measure(s)<\/jats:title>\n Gene expression, oxygen consumption rate (OCR), extracellular acidification rate (ECAR), and cell proliferation<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n PLIN2 gene expression was 2.4-fold lower in leiomyoma compared to adjacent myometrium, suggesting a link between PLIN2 deficiency and fibroids. 3877 genes were differentially expressed after PLIN2 knockdown. Gene Ontology analysis identified metabolism as the second highest biological process affected by PLIN2 depletion (P=1.6E-10). OCR (mitochondrial respiration) and ECAR (glycolysis) were significantly upregulated after PLIN2 knockdown; PLIN2 depleted cells had greater basal metabolic activity (P<.0001) and a higher metabolic stress response (P<.0001). Cell proliferation was also significantly increased after PLIN2 knockdown (P<.05).<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n PLIN2 depletion increases mitochondrial respiration and glycolysis, suggesting that PLIN2 is a critical regulator of metabolic function in leiomyoma cells. PLIN2 deficiency also reprograms leiomyoma cells to a pro-proliferative phenotype. These findings introduce metabolomics as a novel area to explore to better understand leiomyoma tumorigenesis.<\/jats:p>\n <\/jats:sec>","DOI":"10.1210\/jc.2019-00762","type":"journal-article","created":{"date-parts":[[2019,7,23]],"date-time":"2019-07-23T12:49:03Z","timestamp":1563886143000},"source":"Crossref","is-referenced-by-count":5,"title":["PLIN2 Functions as a Novel Link between Progesterone Signaling and Metabolism in Uterine Leiomyoma Cells"],"prefix":"10.1210","author":[{"given":"Ijeoma","family":"Okeigwe","sequence":"first","affiliation":[{"name":"Division of Reproductive Science in Medicine, Department of Obstetrics and Gynecology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serdar","family":"Bulun","sequence":"additional","affiliation":[{"name":"Division of Reproductive Science in Medicine, Department of Obstetrics and Gynecology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shimeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Reproductive Science in Medicine, Department of Obstetrics and Gynecology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfred W","family":"Rademaker","sequence":"additional","affiliation":[{"name":"Department of Preventive Medicine, Feinberg School of Medicine, Northwestern University, Chicago, Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John S","family":"Coon V","sequence":"additional","affiliation":[{"name":"Division of Reproductive Science in Medicine, Department of Obstetrics and Gynecology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacy","family":"Kujawa","sequence":"additional","affiliation":[{"name":"Division of Reproductive Science in Medicine, Department of Obstetrics and Gynecology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jared","family":"Robins","sequence":"additional","affiliation":[{"name":"Division of Reproductive Science in Medicine, Department of Obstetrics and Gynecology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Yin","sequence":"additional","affiliation":[{"name":"Division of Reproductive Science in Medicine, Department of Obstetrics and Gynecology"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"80","published-online":{"date-parts":[[2019,7,23]]},"container-title":["The Journal of Clinical Endocrinology & Metabolism"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jcem\/advance-article-pdf\/doi\/10.1210\/jc.2019-00762\/28969448\/jc.2019-00762.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"}],"deposited":{"date-parts":[[2019,9,24]],"date-time":"2019-09-24T20:47:44Z","timestamp":1569358064000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jcem\/advance-article\/doi\/10.1210\/jc.2019-00762\/5536619"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,23]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1210\/jc.2019-00762","relation":{},"ISSN":["0021-972X","1945-7197"],"issn-type":[{"value":"0021-972X","type":"print"},{"value":"1945-7197","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,23]]},"article-number":"jc.2019-00762"}}