{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T20:12:18Z","timestamp":1779912738379,"version":"3.53.1"},"reference-count":56,"publisher":"The Endocrine Society","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1997,12,1]]},"abstract":"<jats:p>A multiplicity of TSH receptor autoantibodies (TSHRAbs) have been characterized after subcloning heterohybridomas produced from the lymphocytes of a patient who has Hashimoto\u2019s thyroiditis and had three children with intrauterine or neonatal hyperthyroidism. Twelve clones produced stimulating TSHRAbs that increased cAMP levels and iodide uptake in rat FRTL-5 thyroid cells and increased cAMP levels in Chinese hamster ovary (CHO) cells transfected with the human TSHR; like 95% of Graves\u2019 stimulating TSHRAbs, all 12 have their functional epitope on the N-terminus of the TSHR extracellular domain, requiring residues 90\u2013165 for activity. All 12 bind to human thyroid membranes in the absence, but not the presence, of TSH, but are only weak inhibitors of TSH binding in assays measuring TSH binding-inhibiting Igs (TBIIs). In contrast, 8 different clones produced TSHRAbs that did not increase cAMP levels, but, instead, exhibited significant TBII activity. Four inhibited the ability of TSH or a stimulating TSHRAb to increase cAMP levels and had their functional epitope on the C-terminal portion of the TSHR external domain, residues 261\u2013370, mimicking the properties of blocking TSHRAbs that cause hypothyroidism in patients with idiopathic myxedema. The 4 other TBIIs inhibited the ability of TSH, but not that of a stimulating TSHRAb, to increase cAMP levels, like TBIIs in Graves\u2019 patients. The functional epitope for 3 of these Graves\u2019-like TBIIs was residues 90\u2013165; the functional epitope for the fourth was residues 24\u201389. The fourth also increased arachidonic acid release and inositol phosphate levels in FRTL-5 thyroid cells and exhibited conversion activity, i.e. the ability to increase cAMP levels in the presence of an anti-human IgG. Thus, this TBII exhibited signal transduction activity, unlike the other 3 Graves\u2019-like TBIIs. The patient, therefore, has stimulating TSHRAbs and 3 different types of TBIIs, each with different functional properties and different epitopes on the TSHR.<\/jats:p>","DOI":"10.1210\/jcem.82.12.4433","type":"journal-article","created":{"date-parts":[[2014,1,8]],"date-time":"2014-01-08T17:01:01Z","timestamp":1389200461000},"page":"3998-4009","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of Monoclonal Thyroid-Stimulating and Thyrotropin Binding-Inhibiting Autoantibodies from a Hashimoto\u2019s Patient Whose Children Had Intrauterine and Neonatal Thyroid Disease"],"prefix":"10.1210","volume":"82","author":[{"given":"Leonard D.","family":"Kohn","sequence":"first","affiliation":[{"name":"Cell Regulation Section, Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (L.D.K., W.H.H., D.T., C.M., K.S.), Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Cell Regulation Section, Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (L.D.K., W.H.H., D.T., C.M., K.S.), Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William H.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Cell Regulation Section, Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (L.D.K., W.H.H., D.T., C.M., K.S.), Bethesda, Maryland 20892"},{"name":"the Department of Pediatrics, Section of Pediatric Endocrinology, Medical College of Georgia (W.H.H.), Augusta, Georgia 30912"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatella","family":"Tombaccini","sequence":"additional","affiliation":[{"name":"Cell Regulation Section, Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (L.D.K., W.H.H., D.T., C.M., K.S.), Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Marcocci","sequence":"additional","affiliation":[{"name":"Cell Regulation Section, Metabolic Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (L.D.K., W.H.H., D.T., C.M., K.S.), Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Shimojo","sequence":"additional","affiliation":[{"name":"the Department of Pediatrics, Chiba University School of Medicine (N.S., Y.K.), Chiba 260, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukihiko","family":"Watanabe","sequence":"additional","affiliation":[{"name":"SRL, Inc. 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patients.","volume":"180","author":"Kosugi","year":"1991","journal-title":"Biochem Biophys Res Commun"},{"key":"2020071620562448200_R34","first-page":"168","article-title":"Identification of separate determinants on the thyrotropin receptor reactive with Graves\u2019 thyroid stimulating antibodies and with thyroid stimulating blocking antibodies in idiopathic myxedema: these determinants have no homologous sequence on gonadotropin receptors.","volume":"6","author":"Kosugi","year":"1992","journal-title":"Mol Endocrinol"},{"key":"2020071620562448200_R35","first-page":"114","article-title":"Identification of thyroid stimulating antibody-specific interaction sites in the N-terminal region of the thyrotropin receptor.","volume":"7","author":"Kosugi","year":"1993","journal-title":"Mol Endocrinol"},{"key":"2020071620562448200_R36","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1073\/pnas.78.4.2145","article-title":"Topographic gradient of molecules in retina can be used to identify neuron position.","volume":"78","author":"Trisler","year":"1981","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071620562448200_R37","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1210\/jcem-57-4-782","article-title":"Characterization of the optimal stimulatory effects of Graves\u2019 monoclonal and serum immunoglobulin G on adenosine 3\u2032,5\u2032-monophosphate production in FRTL-5 thyroid cells; a potential clinical assay.","volume":"57","author":"Vitti","year":"1983","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071620562448200_R38","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/0022-1759(84)90274-6","article-title":"Rapid and efficient purification of mouse monoclonal antibodies from ascites fluid using high performance liquid chromatography.","volume":"69","author":"Burchiel","year":"1984","journal-title":"J Immunol Methods"},{"key":"2020071620562448200_R39","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1128\/JCM.17.4.655-665.1983","article-title":"Principles, problems, and strategies in the use of antigenic mixtures for the enzyme-linked immunosorbent assay.","volume":"17","author":"Kenny","year":"1983","journal-title":"J Clin Microbiol"},{"key":"2020071620562448200_R40","doi-asserted-by":"crossref","first-page":"8467","DOI":"10.1073\/pnas.89.18.8467","article-title":"Human monoclonal islet cell antibodies from a patient with insulin-dependent diabetes mellitus reveal glutamate decarboxylase as the target antigen.","volume":"89","author":"Richter","year":"1992","journal-title":"Proc Natl Acad Sci USA"},{"key":"2020071620562448200_R41","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/S0022-1759(83)80001-5","article-title":"Immunodot assay for determining the isotype and light chain type of murine monoclonal antibodies in unconcentrated hybridoma culture supernates.","volume":"63","author":"McDougal","year":"1983","journal-title":"J Immunol Methods"},{"key":"2020071620562448200_R42"},{"key":"2020071620562448200_R43"},{"key":"2020071620562448200_R44","first-page":"1164","article-title":"Receptor crosstalk can optimize assays for autoantibodies to the thyrotropin receptor: effect of phenylisopropyladenosine on cAMP and inositol phosphate levels in rat FRTL-5 thyroid cells.","volume":"77","author":"Hidaka","year":"1993","journal-title":"J Clin Endocrinol Metab"},{"key":"2020071620562448200_R45","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/BF03348346","article-title":"Graves\u2019 IgG stimulation of iodide uptake in FRTL-5 rat thyroid cells: a clinical assay complementing FRTL-5 rat assays measuring adenylate cyclase and growth-stimulating antibodies in autoimmune thyroid disease.","volume":"6","author":"Marcocci","year":"1983","journal-title":"J Endocrinol 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